We narrowed to 12,214 results for: LEA
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Plasmid#176276PurposeViral vector for co-expression of a CDS of interest and EGFP in cells expressing Cre AND NOT Flp driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.DepositorInsertMCS-P2A-EGFP
UseAAV and Cre/LoxExpressionMammalianPromoterhuman Synapsin IAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-HA3-STONIN1
Plasmid#177580PurposeExpresses Stonin1 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSTONIN1
Tags3XHA and GFPExpressionMammalianMutationV334A- please see depositor commentsAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV_EE-nCas9
Plasmid#163541PurposeMammalian CG-to-GC base editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEE-nCas9
UseCRISPRExpressionMammalianMutationnCas9 (D10A)EE (R126E, R132E)Available sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-SK
Plasmid#136577PurposeDox-inducible polycistronic lentiviral vector expressing mouse Sox2, Klf4DepositorUseLentiviralExpressionBacterialPromotertetO-miniCMV (dox-inducible)Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-SM
Plasmid#136578PurposeDox-inducible polycistronic lentiviral vector expressing mouse Sox2, cMycDepositorUseLentiviralExpressionBacterialPromotertetO-miniCMV (dox-inducible)Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-GFI1
Plasmid#139813Purposedoxycycline-inducible expression of Human GFI1 in mammalian cellsDepositorInsertGFI1 (GFI1 Human)
UseLentiviralExpressionMammalianMutationP2L- Please see depositor comment belowPromoterTRE-mCMVAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6Flag2AUF1(p42)
Plasmid#135941PurposeExpresses AUF1p42 in E. coliDepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
TagsFlag2 and His6ExpressionBacterialPromoterT7Available sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-pEF1a-FLEX-HA-VHH-KASH-WPRE
Plasmid#129704PurposeExpress HA-KASH for nuclei labeling and purificationDepositorInsertHA-VHH-KASH
UseAAV and Cre/LoxTagsHAExpressionMammalianPromoterEF1aAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLR5-CAG-NLS-tdiRFP670
Plasmid#82621PurposeExpresses NLS-tdiRFP670 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-tdiRFP670
UsePiggybac vectorExpressionMammalianAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-7
Plasmid#126055PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR-P2A-BlastR
Plasmid#98147PurposeExpresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica.DepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsP2A-BlastR fusion with Cas9-NluxPromoterRibi promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Sec61B
Plasmid#120244PurposeExpresses V5-tagged Sec61B in lentiviral vectorDepositorAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
6CCR (GID4)
Plasmid#119960PurposeBacterial expression for structure determinationDepositorInsertGID4 (C17orf39 Human)
Tags6xHIS, thrombin,SUMO and TEV cleavageExpressionBacterialMutationcontains amino acids 116-300PromoterT7Available sinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pICSL50005
Plasmid#117536PurposeLevel0 Golden Gate part: c-terminal tag, YFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertc-term tag YFP
UseSynthetic BiologyMutationG65S, L68V, A72S, Y203T, L231HAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
KEAP1 (KEAP1A-c029)
Plasmid#110247PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTag002 DA-MBP
Plasmid#102832PurposeBacterial expression of unreactive version of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002 DA-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationReactive aspartic acid has been mutated to an ala…PromoterT7Available sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJExpress414_GmPOPB-S577A
Plasmid#92235PurposeExpress S577A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-S577A mutant
Tags6x-Histag, TEV cleavage siteExpressionBacterialMutationSerine 577 to AlaninePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC43-FIT2
Plasmid#96993PurposeExpress GFP-tagged mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
TagsGFPExpressionPlantMutationE2D (please see depositor comments below)Promoter35SAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
5FAI (EMG1)
Plasmid#79519PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable sinceJune 15, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTriEx-mCherry-LINXb3
Plasmid#73615PurposeLight-induced nuclear export switch for microscopy in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLINXb3
TagsmCherryExpressionBacterial, Insect, and Mamm…Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only