We narrowed to 13,725 results for: cas9

1. pCAG-Rad18

   Plasmid

   #119003

   Purpose

   Mammalian expression of human Rad18 and BFP

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   Rad18
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKEW215_AcrIIA13

   Plasmid

   #138281

   Purpose

   Expression of AcrIIA13 protein

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   AcrIIA13
   

   Use

   CRISPR

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSABEd

   Plasmid

   #133968

   Purpose

   Expresses ABEd CRISPR base editor and gRNA scaffold in Streptomyces

   Depositor

   Yuhui Sun

   Article

   Zhong et al bioRxiv 630137

   Insert

   ABEd
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. BCJJ180

   Plasmid

   #117516

   Purpose

   Cas9_4 Golden Gate Level 0

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BsaI:AATG:Cas9_4:GCTT:BsaI
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pICSL12015

   Plasmid

   #117506

   Purpose

   AtUBI10 promoter Golden Gate Level 0

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BsaI:GGAG:pUBI10:AATG:BsaI
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtUBI10

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. vHB179

   Plasmid

   #193458

   Purpose

   3xFLAG endogenous tagging of pandoravirus/mollivirus genes

   Depositor

   Chantal Abergel

   Article

   Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

   Insert

   3xFLAG
   

   Use

   Pandoravirus/mollivirus expression

   Tags

   Expression

   Mutation

   Promoter

   ---

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pICSL90016

   Plasmid

   #117514

   Purpose

   Cas9_2 Golden Gate Level 0

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BsaI:AATG:Cas9_2:GCTT:BsaI
   

   Use

   CRISPR

   Tags

   NLS_SV40

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSCBE2

   Plasmid

   #133965

   Purpose

   Expresses BE2 CRISPR base editor and gRNA scaffold in Streptomyces

   Depositor

   Yuhui Sun

   Article

   Zhong et al bioRxiv 630137

   Insert

   BE2
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAH01SpR

   Plasmid

   #128159

   Purpose

   pCAH01 derivative (Ptet inducible promoter) with ahp (kanamycin resistance) CDS replaced with the aadA (streptomycin/spectinomycin resistance) CDS

   Depositor

   Calvin Henard

   Article

   Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. YN2_1_IL50_HOlocus

    Plasmid

    #194426

    Purpose

    Expresses Cas9 and sgRNA cassettes for targeting the HO locus in yeast Saccharomyces cerevisiae

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    NA

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSABEn

    Plasmid

    #133969

    Purpose

    Expresses ABEn CRISPR base editor and gRNA scaffold in Streptomyces

    Depositor

    Yuhui Sun

    Article

    Zhong et al bioRxiv 630137

    Insert

    ABEn
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCF525-AcrIIA5_gb36_lenti

    Plasmid

    #138306

    Purpose

    Lenti expression of AcrIIA5

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA5
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMLS236

    Plasmid

    #73684

    Purpose

    SapTrap 3-site Destination vector for internal GFP tagging with embedded Cbr-unc-119 selectable marker

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    GFP + Cbr-unc-119
    

    Use

    CRISPR and Cre/Lox

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. U6>XX

    Plasmid

    #87542

    Purpose

    Component 1 of 2 for U6>sgRNA cassette OSO-PCR

    Depositor

    Lionel Christiaen

    Article

    Gandhi et al Dev Biol. 2017 May 1;425(1):8-20. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. lentiGuide-Puro.3xBsmBI

    Plasmid

    #196709

    Purpose

    Backbone for cloning guide libraries with untranscribed barcodes and a tracrRNA of choice

    Depositor

    Bernhard Schmierer

    Article

    Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMLS235

    Plasmid

    #73683

    Purpose

    SapTrap 3-site Destination vector for C-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    GFP + Cbr-unc-119
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AP682-1

    Plasmid

    #70050

    Purpose

    Expresses TEV::eGFP::myc::3Xflag in bacteria

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

    Insert

    eGFP
    

    Use

    Tags

    TEV and myc::3Xflag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDD399

    Plasmid

    #91821

    Purpose

    AID CT-tag linker donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Auxin-inducible degron (AID)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMLS360

    Plasmid

    #73687

    Purpose

    Punc-47::2xNLS-FLP-D5 Expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    Punc-47::2xNLS-FLP-D5::let-858 3'-UTR
    

    Use

    Flp/frt

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Punc-47

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCfB8383

    Plasmid

    #126917

    Purpose

    Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of tHMG1

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    tHMG1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge