We narrowed to 18,681 results for: tec

1. pVH613

   Plasmid

   #169602

   Purpose

   Tier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (PCREm4-Nluc-pA::A2-pA::PmPGK1-Fluc-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   cAMP response element-controlled NanoLuc Luciferase expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PCREm4 / PPGK

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-4T-1-p19-T7-PLK1

   Plasmid

   #46312

   Depositor

   Judy Lieberman

   Article

   Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

   Inserts

   p19
   PLK1 DNA-1
   linker
   PLK1 DNA-2
   

   Use

   Pro-sirna

   Tags

   6XHis and GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-b2AR-hLOV1-TEVcs-FLAG-GAL4-V5

   Plasmid

   #104842

   Purpose

   b2AR-SPARK TF component

   Depositor

   Alice Ting

   Article

   Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

   Insert

   hLOV1-TEVcs-FLAG-GAL4-V5
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pconst #1-RFP

   Plasmid

   #81075

   Purpose

   constitutive promoter expressing RFP, biobrick construction

   Depositor

   Jay Keasling, Paul Adams

   Article

   Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

   Insert

   RFP
   

   Use

   P15a origin, constitutive promoter, kanr

   Tags

   Expression

   Mutation

   Promoter

   Constitutive Promoter - BBa_J23119

   Available since

   Aug. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfB6677

   Plasmid

   #106155

   Purpose

   EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6632, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS416-Aga1p-FAPB2.3.6

   Plasmid

   #158147

   Purpose

   Yeast display reporter for non-display yeast strains (wild-type construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   Aga1p-FAPB2.3.6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15b+PCNA1 P186C

   Plasmid

   #66173

   Purpose

   Expresses the P186C mutant of Sulfolobus solfataricus PCNA1

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 1
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   P186C

   Promoter

   T7 promoter

   Available since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pILGFPQ4

   Plasmid

   #83559

   Purpose

   Used to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   GAL7 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL7

   Available since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. LLP689_L2_1IF2_FRT-OCS_Rluc_C1_FlpF2

   Plasmid

   #192412

   Purpose

   To act as a 1-input state of the split-AND gate.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. LLP690_L2_1IF1_FRT-OCS_Rluc_FlpF1_C1-NLS

    Plasmid

    #192413

    Purpose

    To act as a 1-input state of the split-AND gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6nH1-HD10kb

    Plasmid

    #172657

    Purpose

    Expressing paired pegRNAs from human U6 and H1 promoters to make 10204-bp deletion on HPRT1 gene

    Depositor

    Jay Shendure

    Article

    Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    pegRNA-HD10kbA/pegRNA-HD10kbB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. TAL2384

    Plasmid

    #36793

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    BRCA1-TALEN_Left (Brca1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pXCX.CMV.CREB.WPRE

    Plasmid

    #38050

    Depositor

    James Uney

    Article

    Glover et al Neuroreport. 2004 May 19;15(7):1171-5.

    Insert

    CREB (Creb1 Rat)
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. TAL2274

    Plasmid

    #36711

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    MDM2-TALEN_Left (Mdm2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pVH72-Tier1-PhCMV-SEAP

    Plasmid

    #169560

    Purpose

    Tier-1 vector encoding PhCMV-driven SEAP expression containing a computationally optimized secretion peptide (PhCMV-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven human secreted placental alkaline phosphatase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAC1748

    Plasmid

    #127982

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, argB selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available since

    Dec. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUCBB-ptet-eGFP

    Plasmid

    #32554

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Mutation

    Promoter

    ptet

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C261

    Plasmid

    #171429

    Purpose

    Expression of FKBP-(GGS)X6-HaloTagM C261 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    FKBP-(GGS)X6-HaloTag C261
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. EK0354 SFFV-mCherry-SG(Bdnf.s1L72)SG-EGFP (FLP-IN)

    Plasmid

    #191151

    Purpose

    Expression of a 72 bp long sensor for murine Bdnf 3' UTR in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:Bdnf.s1L72:EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAPM-D4-miR30-TRIM5

    Plasmid

    #132932

    Purpose

    Lentivector for TRIM5 knockdown and puromycin resistance

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    TRIM5 shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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