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spNW100

Plasmid

#173488

Purpose

Expresses spNW100 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW100

Tags

Histag

Expression

Bacterial

Mutation

P1049L- please see depositor comments

Promoter

T7

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-α-cateninA-

Plasmid

#229698

Purpose

Transient expression of GFP-alpha-cateninA- in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

L785A, I792A, V796A

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pWPXL-p150

Plasmid

#160992

Purpose

Express fulllength of mouse p150 protein

Depositor

Zhiguo Zhang

Article

Cheng et al Nucleic Acids Res. 2019 Dec 2;47(21):111

Insert

CHAF1A (Chaf1a Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-TFE3

Plasmid

#38120

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFE3 (TFE3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only

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mDlx-SynTagMA_post-2A-mCerulean

Plasmid

#130899

Purpose

Synaptic Tag for Mapping Activity in GABAergic neurons

Depositor

Simon Wiegert

Article

Perez-Alvarez et al Nat Commun. 2020 May 18;11(1):2464. doi:

Insert

SynTagMA

Use

AAV

Tags

mCerulean

Expression

Mammalian

Promoter

mDlx

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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eGFP Y93H CC editing reporter

Plasmid

#198885

Purpose

Express eGFP fluorescence after Y93H correction and mCherry as transfection control

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Mutation

Y93H, Gly92(GGT-to-GGC)

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGEM4Z-T7-5'UTR-mGreenLantern-3'UTR-A64

Plasmid

#203349

Purpose

Template for T7 in vitro transcription of mGreenLantern mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail

Depositor

Christopher Grigsby, Molly Stevens

Insert

mGreenLantern

Use

Template for t7-mediated in vitro transcription o…

Tags

Flanked by 5' and 3' UTRs from Xenopus …

Promoter

T7

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pRPF185

Plasmid

#106367

Purpose

C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

Depositor

Robert Fagan, Neil Fairweather

Article

Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

Insert

Term(fdx)-Ptet-gusA-Term(slpA) (gusA )

Use

E. coli - c. difficile shuttle vector

Mutation

codon optimised for C. difficile

Promoter

Ptet

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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Bin1 delta N-BAR

Plasmid

#168282

Purpose

Express N-BAR-deleted Bin1 mutant (aa 268-476)

Depositor

Cheng-han Yu

Article

Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

Insert

Bin1 (Bin1 Mouse)

Expression

Mammalian

Mutation

aa 268-476

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pKMW281_dSpRY

Plasmid

#244822

Purpose

Mammalian expression of catalytically inactive SpRY Cas9 (dSpRY)

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

dSpRY Cas9

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Mutation

D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

Promoter

CAG

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt5a

Plasmid

#42279

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt5a (Wnt5a Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
lenti-SpCas9 puro

Plasmid

#104994

Purpose

This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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EF.CMV.RFP

Plasmid

#17619

Depositor

Linzhao Cheng

Article

Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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JDW 1043 (pLenti6.2_CMV_V5_LEF1_P2A_H2A_mCherry)

Plasmid

#233538

Purpose

A CMV driven human LEF1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

LEF1 (LEF1 Human)

Use

Lentiviral

Tags

V5

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pPyPGK-Myc-LaG17-SynNotch-tTA-IRES-Ble

Plasmid

#183607

Purpose

Mammalian expression vector: mouse Pgk1 promoter driving expression of a SynNotch receptor comprising anti-GFP nanobody (LaG17), mouse Notch1 core, tTA. Confers bleomycin/zeocin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

LaG17 GFP nanobody SynNotch tTA

Tags

Myc and human CD8a signal sequence

Expression

Mammalian

Promoter

Mouse Pgk1

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-(-30)GFP

Plasmid

#62936

Purpose

Expression of 6xHis-(-30)GFP in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(-30)GFP

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pLViN-iRFP670-α-cateninWT

Plasmid

#229702

Purpose

Lentiviral expression of iRFP670-alpha-cateninWT in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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3xERRE/ERE-luciferase

Plasmid

#37852

Depositor

Rebecca Riggins

Article

Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

Insert

3 copies of ERRE/ERE upstream of luciferase

Use

Luciferase

Promoter

SV40

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-MyD88

Plasmid

#12287

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

MyD88 (MYD88 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only

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pB-3xFlag-dCas13-mTET2HxDCD

Plasmid

#228235

Purpose

For targeted tethering of an inactive mutant (HxD) of the catalytic domain of mouse TET2 using dCas13

Depositor

Chuan He

Article

Zou et al Nature. 2024 Oct 2. doi: 10.1038/s41586-

Inserts

dCas13
TET2 (Tet2 Mouse)

Use

CRISPR

Expression

Mammalian

Mutation

CD (cysteine-rich, dioxygenase domain) truncation…

Promoter

CAG

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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