We narrowed to 13,725 results for: cas9

1. pCMV-T7-SpG-P2A-mScarlet (MNW006)

   Plasmid

   #174136

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

   Promoter

   CMV and T7

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. lentiCRISPRv2-7SK-Blast

   Plasmid

   #183620

   Purpose

   lentiCRISPRv2 derivative with modified 7SK promoter driving sgRNA and encoding Blasticidin resistance. Compatible with inserts designed for lentiCRISPRv2.

   Depositor

   Bill Sugden

   Article

   Hutcheson et al ACS Synth Biol. 2022 Jun 29. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Modified 7SK

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMLS719

   Plasmid

   #188894

   Purpose

   miniMos Arm sgRNA expression vector

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   PU6(K09B11.12)::miniMos Arm sgRNA
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDD375

   Plasmid

   #91825

   Purpose

   Codon-optimized mKate2 donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   mKate2-C1
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCfB8622

   Plasmid

   #126912

   Purpose

   Plasmid containing gRNA expression cassette targeting ADE2 in Saccharomyces cerevisiae

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   ADE2 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. U6>Ebf.774(F+E)

   Plasmid

   #59990

   Purpose

   U6 promoter driving Ebf.774 (F+E) sgRNA

   Depositor

   Lionel Christiaen

   Article

   Stolfi et al Development. 2014 Nov;141(21):4115-20. d

   Insert

   Ebf.774 (F+E) sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ciinte.U6

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAP635-2

   Plasmid

   #99485

   Purpose

   Myc::TEV::3XFlag::Dendra2::3XFlag::TEV::Myc

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   Myc::TEV::3XFlag::Dendra2::3XFlag::TEV::Myc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. YN2_1_LT84_RPL13A

   Plasmid

   #177712

   Purpose

   Expresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiae

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDD397

   Plasmid

   #91822

   Purpose

   AID FP-slot donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Auxin-inducible degron (AID)
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LBJJ432

    Plasmid

    #117508

    Purpose

    AtEC_enh. promoter Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:GGAG:pEC_enh:AATG:BsaI
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    EC1.2:EC1.1

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDD398

    Plasmid

    #91832

    Purpose

    AID NT-tag linker donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Auxin-inducible degron (AID)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAP685-2

    Plasmid

    #99490

    Purpose

    TEV::eGFP::ER::6xHis::linker::3XFlag

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    TEV::eGFP::RE::6Xhis::linker::3XFlag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AByF

    Plasmid

    #111084

    Purpose

    Integrates marker gene onto D. melanogaster Y chromosome

    Depositor

    Omar Akbari

    Article

    Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

    Inserts

    Y homology arm left
    gypsy insulator
    3xP3 promoter
    tdTomato
    SV40 3'UTR
    CTCF insulator
    Y homology arm right
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCfB8382

    Plasmid

    #126916

    Purpose

    Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTLR-donor-UAS

    Plasmid

    #119001

    Purpose

    Repair template for the TLR reporter with 10 UAS binding sites for yeast Gal4

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    mVenus
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTLR-donor-tetO

    Plasmid

    #119000

    Purpose

    Repair template for the TLR reporter with 7 tet operator elements

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    mVenus
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCF525-AcrIIA13b_gb76_GF31-2-hom_lenti

    Plasmid

    #138310

    Purpose

    Lenti expression of AcrIIA13b

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA13b
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCF525-AcrIIA15_gb79_Cand27_lenti

    Plasmid

    #138313

    Purpose

    Lenti expression of AcrIIA15

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA15
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCF525-AcrIIA14_gb77_Cand9_lenti

    Plasmid

    #138311

    Purpose

    Lenti expression of AcrIIA14

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA14
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKER-mCardinal

    Plasmid

    #85976

    Purpose

    Expresses mCardinal from the EF1a promoter in mammalian cells

    Depositor

    Michele Calos

    Article

    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

    Insert

    mCardinal
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge