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NK601

Plasmid

#109070

Purpose

Parental vector for making S. rosetta expression vectors

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_03784

Use

Choanoflagellate expression

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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RCAS IkB alpha M

Plasmid

#12407

Depositor

Inder Verma

Insert

IkB alpha M (Nfkbia Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Dominant-negative mutant. Many signal transductio…

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
NK590

Plasmid

#109084

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

mPapaya

Use

Choanoflagellate expression

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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NK594

Plasmid

#109085

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

TagRFP-T

Use

Choanoflagellate expression

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19 gene 60

Plasmid

#49090

Purpose

Template for generating full length gene 60 mRNA by in vitro transcription with T7 RNA pol

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Promoter

T7

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

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NK649

Plasmid

#109105

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_06009

Use

Choanoflagellate expression

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCD005

Plasmid

#113314

Purpose

W108 scRNA

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

W108 scRNA_MS2

Use

CRISPR

Expression

Bacterial

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCRB DRT7VTPLux*500YFP

Plasmid

#107961

Purpose

YFP half-circuit controller plasmid [ca. 558.5 relative RBS strength at PLas81*]

Depositor

Jim Haseloff

Article

Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

Insert

T7RNAP (modified)

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCRB DRT7VTPLux*500CFP

Plasmid

#107960

Purpose

CFP half-circuit controller plasmid [ca. 558.5 relative RBS strength at PLas81*]

Depositor

Jim Haseloff

Article

Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

Insert

T7RNAP (modified)

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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NK619

Plasmid

#109089

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

mCherry

Use

Choanoflagellate expression

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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NK611

Plasmid

#109088

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

mCherry

Use

Choanoflagellate expression

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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NK610

Plasmid

#109087

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

mCherry

Use

Choanoflagellate expression

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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synNANOS

Plasmid

#242616

Purpose

TMP-inducible strong RNA regulator (tethered to MCP)

Depositor

Lacra Bintu

Article

Thurm et al bioRxiv [Preprint]. 2024 Jul 19:2024.07.

Insert

NANOS1 fragment

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGEL1021

Plasmid

#225273

Purpose

Cas12i1-KRAB_SRDX system backbone for gene regulation in rice

Depositor

Yong Zhang

Article

He et al Plant Physiol. 2025 Apr 30;198(1):kiaf12

Type

Empty backbone

Expression

Plant

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pGEL1020

Plasmid

#225272

Purpose

Cas12i1-TAD system backbone (Cas12i1-TV) for gene regulation in rice

Depositor

Yong Zhang

Article

He et al Plant Physiol. 2025 Apr 30;198(1):kiaf12

Type

Empty backbone

Expression

Plant

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pJethlpAmScarletKm

Plasmid

#206820

Purpose

Template for hlpA-mScarlet-I amplification associated with kanamycin resistance gene

Depositor

Virginie Libante

Article

Mignolet et al bioRxiv 2025.05.10.653270

Insert

hlpA

Use

Synthetic Biology

Tags

mScarlet

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pJethlpAmNeonGreenSpc

Plasmid

#206817

Purpose

Template for hlpA-mneongreen amplification associated with spectinomycin resistance gene

Depositor

Virginie Libante

Article

Mignolet et al bioRxiv 2025.05.10.653270

Insert

hlpA

Use

Synthetic Biology

Tags

mNeonGreen

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pJethlpAmScarletEry

Plasmid

#206819

Purpose

Template for hlpA-mScarlet-I amplification associated with erythromycin resistance gene

Depositor

Virginie Libante

Article

Mignolet et al bioRxiv 2025.05.10.653270

Insert

hlpA

Use

Synthetic Biology

Tags

mScarlet

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pJethlpAmScarletSpc

Plasmid

#206821

Purpose

Template for hlpA-mScarlet-I amplification associated with spectinomycin resistance gene

Depositor

Virginie Libante

Article

Mignolet et al bioRxiv 2025.05.10.653270

Insert

hlpA

Use

Synthetic Biology

Tags

mScarlet

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pJethlpAmTurquoise2Cm

Plasmid

#206822

Purpose

Template for hlpA-mTurquoise2 amplification associated with chloramphenicol resistance gene

Depositor

Virginie Libante

Article

Mignolet et al bioRxiv 2025.05.10.653270

Insert

hlpA

Use

Synthetic Biology

Tags

mTurquoise2

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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