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hPCNA-C148S

Plasmid

#190939

Purpose

untagged human PCNA with a cysteine to serine mutation

Depositor

Brian Kelch

Article

Magrino et al J Biol Chem. 2023 May;299(5):104656. doi

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Expression

Bacterial

Mutation

changed cysteine 148 to serine

Promoter

T7

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-ATG13 (2-197)-R153D

Plasmid

#188104

Purpose

Expresses human ATG13 HORMA R153D mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Tags

strep-strep-Flag tag

Expression

Mammalian

Mutation

ATG13 (2-197)-R153D

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_KLF4

Plasmid

#140104

Purpose

CRISPR-Cas9 library validation

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Promoter

gRNA1 under U6 and gRNA2 under H1

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-INTS3-C

Plasmid

#128642

Purpose

Express INTS3 C-termini (514-1042 aa) in E. coli with N-terminal and C-terminal His tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 C-termini (INTS3 Human)

Tags

His tag on N and C-terminal

Expression

Bacterial

Mutation

INTS3 C-termini (514–1042 aa)

Promoter

T7

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLX304 PCSK5 (R511C)-V5 blast

Plasmid

#83104

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (R511C) with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Arginine 511 to Cysteine

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
gh67

Plasmid

#106738

Purpose

expression of gRNA targeting B3GNT1 (B4GAT1)

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B3GNT1 (B4GAT1) (B4GAT1 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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PLAU gRNA (BRDN0001162221)

Plasmid

#77104

Purpose

3rd generation lentiviral gRNA plasmid targeting human PLAU

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PLAU (PLAU Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PA-mCherry-miniSOG-Keratin-17

Plasmid

#57792

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Keratin (KRT18 Human)

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only
PA-mCherry-miniSOG-Alpha-Actinin-19

Plasmid

#57782

Purpose

Localization: Alpha Actinin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Alpha-Actinin (ACTN1 Human)

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
PA-mCherry-miniSOG-EB3-7

Plasmid

#57788

Purpose

Localization: MT End Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

EB3 (MAPRE3 Human)

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only