We narrowed to 29,757 results for: REP
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Plasmid#225661PurposeLentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (red configuration)DepositorInsertdsCherry
UseLentiviralTagsExpressionMutationPromoterSORE6-mCMVpAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDblet
Plasmid#8848DepositorInsertARS doublet
UseYeast cloning vectorTagsExpressionYeastMutationThis vector contains, between AatII sites, a dire…PromoterAvailable SinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMX-eSOX17WHC
Plasmid#118839PurposeRetroviral expression of a mutant of Sox17 that improves efficiency of iPSC generationDepositorInserteSOX17WHC (Sox17 Mouse)
UseRetroviralTagsExpressionMammalianMutationWHC: L46W, V53H, E57CPromoterAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGM55
Plasmid#71249Purposeluciferase reporter with hGM-CSF -55 to +28 minimal promoterDepositorInsertGM-CSF minimal promoter (-55 to +28) (CSF2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterGM-CSF minimalAvailable SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS306Sup35R1
Plasmid#17715DepositorInsertpRS306Sup35R1 (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationAn pRS306 based integrating plasmid designed for …PromoterAvailable SinceApril 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
LIG4 HAL-T2A-GFP-SV40pA-HAR
Plasmid#225357PurposeThis plasmid serves as a template for homologous DNA recombination at the LIG4 gene to knock out the DNA ligase 4 protein using the SelectRepair knockout GFP approach.DepositorInsertT2A-GFP-SV40pA
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
LIG4 HAL-T2A-HYG-SV40pA-HAR
Plasmid#225358PurposeThis plasmid serves as a template for homologous DNA recombination at the LIG4 gene to knock out the DNA ligase 4 protein using the hygromycin B SelectRepair knockout approach.DepositorInsertT2A-HYG-SV40pA
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
LIG4 HAL-T2A-NEO-SV40pA-HAR
Plasmid#225359PurposeThis plasmid serves as a template for homologous DNA recombination at the LIG4 gene to knock out the DNA ligase 4 protein using the neomycin SelectRepair knockout approach.DepositorInsertT2A-NEO-SV40pA
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
p11d-RPA70/14 (MSW#134)
Plasmid#208076PurposeExpression of human Replication Protein A 70 and 14-kDa subunits in E. coli with 14 having a His-tagDepositorAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM5Z(+)-EGFP
Plasmid#65206PurposeEGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.DepositorInsertEGFP
UseTagsExpressionBacterial and MammalianMutationRestriction enzyme recognition sites added either…PromoterCMVAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS CMV NA 3C FLAG SA
Plasmid#234993PurposeExpression of transmembrane region of Neuraminidase protein fused to streptavidin protein for displaying on viral like particles (VLPs) when cotranfected with GAG proteinDepositorInsertNA
UseTagsHRV 3C site, FLAG, Strep-Tactin (SA)ExpressionMammalianMutationIn the Streptavidin coding sequence Glu44Val/Ser4…PromoterAvailable SinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsRPA32 (MSW#497)
Plasmid#208068PurposeExpression of GFP-tagged hsRPA2 in human cellsDepositorInsertRPA2 (RPA2 Human)
UseTagsGFPExpressionMammalianMutationT88I- please see depositor commentsPromoterAvailable SinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM5188
Plasmid#120087PurposeCyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.DepositorInsertpAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
UseOtherTagsExpressionMutationPromoterAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal.Sth.dCas9
Plasmid#171088PurposeCRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.DepositorInsertsToxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
DHFR-TSc3
UseToxoplasma gondii expressionTagsExpressionMutationNote: A S245F mutation is present but did not app…PromoterDHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…Available SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUC2::GNSI (NrsR)
Plasmid#121537PurposeThis plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.DepositorInsertsCYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator
NrsR
UseTagsmGFP5ExpressionYeastMutationM109I (Naturally occurring SNP) and The first 21 …PromoterAvailable SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV225
Plasmid#132334PurposedCas9 plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertdCas9-AT-CBF1 (CBF1 Cas9 (Streptococcus pyogenes); AT-CBF1 (Arabidopsis thaliana))
UseTagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…PromoterAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEE052
Plasmid#176809PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert603
UseTags6xHisExpressionBacterialMutationPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PV259
Plasmid#132340PurposeCasD plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertCasD-AT-CBF1 (CBF1 Mustard Weed, CasD (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana))
UseTagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…PromoterAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV503
Plasmid#132342PurposeCasE plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertCasE-AT-CBF1 (CBF1 Mustard Weed, CasE (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana))
UseTagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…PromoterAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV504
Plasmid#132343PurposeCasA plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertCasA-AT-CBF1 (CBF1 CasA (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana), Mustard Weed)
UseTagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…PromoterAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMX-eSOX2AHK
Plasmid#118826PurposeRetroviral expression of a mutant of Sox2 that improves efficiency of iPSC generationDepositorInserteSox2AHK (Sox2 Mouse)
UseRetroviralTagsExpressionMammalianMutationAHK: E46A, I53HPromoterAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMX-eSOX2NRR
Plasmid#118825PurposeRetroviral expression of a mutant of Sox2 that improves efficiency of iPSC generationDepositorInserteSOX2NRR (Sox2 Mouse)
UseRetroviralTagsExpressionMammalianMutationNRR: E46N, I53R, K57RPromoterAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pELPS-eDHFR-YFP-T2A-Renilla
Plasmid#193253PurposeTriple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferaseDepositorInsertThe eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
UseLentiviralTagsRenilla luciferase (rLuc) and yellow fluorescent …ExpressionMutationNonePromoterEF1aAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA(123)
Plasmid#102613PurposeExpression of human RPA in E. coliDepositorUseTagsExpressionBacterialMutationPromoterT7Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCVL-UCOE-SFFV-TLR-MCV1
Plasmid#117111PurposeLentiviral vector encoding Traffic light reporter 2.0. Alternative name: pCVL-UCOE-SFFV-TLR-MCV1.DepositorInsertUCOE SFFV Traffic Light Reporter 2.0
UseLentiviral; ReporterTagsExpressionMammalianMutationPromoterSFFVAvailable SinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEABR NA 3C FLAG SA
Plasmid#234987PurposeFor production of Extracellular Vesicles (EVs), with the transmembrane region of Neuraminidase protein fused to Streptavidin on the vesicles surfaceDepositorInsertEABR-NA
UseTagsHRV 3C site, FLAG, Strep-Tactin (SA)ExpressionMammalianMutationIn the Streptavidin coding sequence Glu44Val/Ser4…PromoterAvailable SinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
M51
Plasmid#242620PurposeStrep-MYC-His protein expression in bacteriaDepositorAvailable SinceSept. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHDR_sox2HA_P2A-GFP-pA_HA_G2913A
Plasmid#196191PurposeTagging human SOX2 gene with P2A-EGFP at the C-terminusDepositorInsertSOX2-HAL-P2A-EGFP-SOX2-HAR (SOX2 Human)
UseHomology repair donorTagsExpressionMutationG2913A mutation to replace the guide cutting site…PromoterAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLD037-pCMV-APOBEC-Cas9(D10A)-rXRCC1
Plasmid#165444PurposeExpresses ACX, rXRCC1 in mammalian cellsDepositorInsertAPOBEC-nCas9-rXRCC1 (Apobec1 Synthetic, Streptococcus pyogenes, Rat)
UseTagsExpressionMammalianMutationPromoterAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHL-EF1a-SphcCas9-iP-A
Plasmid#60599PurposeExpresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.DepositorInsertCRISPR Cas9
UseCRISPRTagsExpressionMammalianMutationCodon usage optimized for human usagePromoterAvailable SinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only