Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 8,446 results for: gnal

Clear keywords
Showing: 7321 - 7350 of 8446 results

  1. MRGPRG-DuET

    Plasmid
    #213343
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MRGPRG (MRGPRG Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213343-plasmid-map-sequence-id-430871

    Enlarge

  2. SSTR5-DuET

    Plasmid
    #213380
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    SSTR5 (SSTR5 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213380-plasmid-map-sequence-id-430735

    Enlarge

  3. SUCNR1-DuET

    Plasmid
    #213381
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    SUCNR1 (SUCNR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213381-plasmid-map-sequence-id-430736

    Enlarge

  4. S1PR4-DuET

    Plasmid
    #213375
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    S1PR4 (S1PR4 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213375-plasmid-map-sequence-id-430731

    Enlarge

  5. SSTR1-DuET

    Plasmid
    #213376
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    SSTR1 (SSTR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213376-plasmid-map-sequence-id-430732

    Enlarge

  6. PTGER1-DuET

    Plasmid
    #213370
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    PTGER1 (PTGER1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213370-plasmid-map-sequence-id-430729

    Enlarge

  7. ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor

    Plasmid
    #173208
    Purpose
    HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I mTOR signaling indicator (TOSI) cassette to ATP1A1 intron 17.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1-T804N mScarlet-I-TOSI donor
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hPGK1
    Available Since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    173208-plasmid-map-sequence-id-339517

    Enlarge

  12. pcDNA3_HLA-cmyc-GRM3 C127A TMD Y616M

    Plasmid
    #140454
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and Y616M mutant
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, tyrosine 616 to methioni…
    Available Since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140454-plasmid-map-sequence-id-292302

    Enlarge

  13. pcDNA3_HLA-cmyc-GRM3 C127A TMD L624N

    Plasmid
    #140455
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and L624N mutant
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, leucine 624 to asparagine
    Available Since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140455-plasmid-map-sequence-id-292306

    Enlarge

  14. pcDNA3_HLA-cmyc-GRM3 C127A TMD A634N

    Plasmid
    #140456
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and A634N mutant
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, alanine 634 to asparagine
    Available Since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140456-plasmid-map-sequence-id-292300

    Enlarge

  15. pCEP4_HLA-flag-GRM3 C127A-VN

    Plasmid
    #140449
    Purpose
    Mammalian expression plasmid for FLAG-tagged mGluR3 fused to the N-terminal half of split fluorescent Venus (VN)
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Tags
    FLAG tag, Signal/leader sequence from HLA class I…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine
    Available Since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140449-plasmid-map-sequence-id-292293

    Enlarge

  16. pCEP4_HLA-myc-GRM3 C127A-VC

    Plasmid
    #140450
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 fused to the C-terminal half of split fluorescent Venus (VC)
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine
    Available Since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140450-plasmid-map-sequence-id-292295

    Enlarge

  17. pcDNA3_HLA-cmyc-GRM3 C127A TMD I578A

    Plasmid
    #140452
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and I578A mutant
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, isoluecine 578 to alanine
    Available Since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140452-plasmid-map-sequence-id-292301

    Enlarge

  18. pcDNA3_HLA-cmyc-GRM3 C127A TMD F588V

    Plasmid
    #140453
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and F588V mutant
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, phenylalanine 588 to val…
    Available Since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140453-plasmid-map-sequence-id-292308

    Enlarge

  22. pTS1046-Tier1-PhCMV-4xCox8-mRuby2

    Plasmid
    #169537
    Purpose
    Tier-1 vector encoding PhCMV-driven mRuby2 localized to the mitochondrial matrix via a four-fold Cox8 localization signal (PhCMV-4xCox8-mRuby2-pA)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven miochondria-localized monomeric red fluorescent protein mRuby2
    Expression
    Mammalian
    Promoter
    PhCMV
    Available Since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    169537-plasmid-map-sequence-id-364564

    Enlarge

  23. pRSET-TorA-6xHis-M13-pHCountdown-Calmodulin

    Plasmid
    #182305
    Purpose
    E. coli plasmid encoding a fusion between pH-Countdown and the calcium-binding domains of GECO 1.2 with a hexahistidine tag, targeted to the periplasm via the TorA presequence.
    Depositor
    Andrew York
    Article
    Seidel et al (2021), doi: 10.5281/zenodo.5810930
    Insert
    pH-Countdown insertion into GECO 1.2 calcium sensing domains
    Tags
    A modified TorA periplasmic targeting sequence pl…
    Expression
    Bacterial
    Mutation
    Spontaneous mutation of calmodulin's penulti…
    Promoter
    t7
    Available Since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    182305-plasmid-map-sequence-id-360536

    Enlarge

  26. pCAG-mSpata33 (I81A)-PA

    Plasmid
    #179131
    Purpose
    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. I81A mutation is introduced in Spata33.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    spermatogenesis associated 33 (Spata33 Mouse)
    Tags
    PA
    Expression
    Mammalian
    Mutation
    Changed Isoleucine 81 to Alanine
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179131-plasmid-map-sequence-id-352818

    Enlarge

  28. pCAG-mSpata33 (P79A)-PA

    Plasmid
    #179130
    Purpose
    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. P79A mutation is introduced in Spata33.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    spermatogenesis associated 33 (Spata33 Mouse)
    Tags
    PA
    Expression
    Mammalian
    Mutation
    Changed Proline 79 to Alanine
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179130-plasmid-map-sequence-id-352804

    Enlarge

  29. pCAG-mSpata33 (I83A)-PA

    Plasmid
    #179132
    Purpose
    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. I83A mutation is introduced in Spata33.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    spermatogenesis associated 33 (Spata33 Mouse)
    Tags
    PA
    Expression
    Mammalian
    Mutation
    Changed Isoleucine 83 to Alanine
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179132-plasmid-map-sequence-id-352797

    Enlarge

  30. pN1mcherry-NKD2G2A

    Plasmid
    #174334
    Purpose
    encodes human NKD2 G2A (2nd Gly residue to Ala) mutant cDNA with a C-terminal mCherry fusion.
    Depositor
    Sonal Srikanth
    Article
    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:
    Insert
    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    Tags
    mCherry tag
    Expression
    Mammalian
    Mutation
    mutation of a.a 2 Gly to Ala
    Promoter
    CMV
    Available Since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174334-plasmid-map-sequence-id-349873

    Enlarge

Showing: 7321 - 7350 of 8446 results