We narrowed to 43,420 results for: cha;
-
Plasmid#121536PurposesgITGB4-3 sequence: GAGGAGCGTAGGTCCTCGCAG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.DepositorInsertsgITGB4-3
UseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromotermouse U6Available sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PARL-FLAG-CT S65D+T69D+S70D
Plasmid#13617DepositorUseTagsFLAGExpressionMammalianMutationchanged Serine 65, Thr 69 and Ser70 to AspPromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
MDH1-miR-181a1-Dusp6
Plasmid#17849DepositorInsertDusp6-EGFP (Dusp6 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMV-α1L9'S-nAchR
Plasmid#20684DepositorInsertnicotinic acetylcholine receptors (Chrna1 Mouse)
UseTagsAMV and pA 50ExpressionBacterialMutationLeucine at 9' position in M2 region changed …PromoterAvailable sinceApril 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
FUW-M2-PAK2 S20A
Plasmid#31664DepositorInsertp21-activated protein kinase 2 S20A (PAK2 Human)
UseLentiviralTagsM2ExpressionMammalianMutationChanged Serine 20 to AlaninePromoterAvailable sinceApril 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pScalps eSpCas9_Zsgreen gRNA scaffold
Plasmid#188449PurposeExpression of eSpCas9 tagged with ZsgreenDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterEF1-alphaAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-mEos4b-V69T
Plasmid#98572Purposephotoconvertible fluorescent protein mEos4b-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpressionDepositorInsertmEos4b-V69T
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB121
Plasmid#68575PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T Doc2beta C2AB(125) WT
Plasmid#134690PurposeEncodes cytoplasmic domain of Doc2beta for bacterial expression and purificationDepositorInsertDouble C2 protein beta (Doc2b Rat)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn_CapChR1_eYFP
Plasmid#188033PurposeExpresses CapChR1-eYFP in mammalian neuronsDepositorInsertCapChR1-eYFP
UseAAVTagseYFPExpressionMutationS63D, L132C, T159C and N258E mutations in CrChR2PromoterhSynAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron 4xGCN4 Renilla FKBP STOP 24xMS2v5 SV40 CTE polyA
Plasmid#104984PurposeExpresses ST-Renilla reporter in mammalian cellsDepositorInsertRenilla luciferase
UseTags24xMS2v5 stem loops in 3'UTR, 4xGCN4 repeats…ExpressionBacterial and MammalianMutationPromoterTetCMVAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
DsRed +9e
Plasmid#191766PurposeExpression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9eDepositorInsertDsRed
UseTagsN terminal fusion of positively charged polypetod…ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
FKBP-YPet-RanGAP*
Plasmid#175245PurposeBacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPetDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationAmino acid 562 F to APromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
OVA-miSFIT-2xPerfect
Plasmid#124673PurposeTuning expression of cytosolic Ovalbumin coupled to EGFPDepositorInsertCyto-Ovalbumin
UseTagsExpressionMammalianMutationTruncated OVA for cytoplasmic expressionPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PARL-FLAG-CT S65A+T69A+S70A
Plasmid#13616DepositorUseTagsFLAGExpressionMammalianMutationchanged Ser 65, Thr 69 and Ser 70 to AlaninePromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCI syn iGluf
Plasmid#106121PurposeFast indicator for synaptic glutamate imaging (synapsin promoter)DepositorInsertiGluSnFR E25D variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Glu 25 to AspPromotersynapsinAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-mKikGR-V69T
Plasmid#98574Purposephotoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coliDepositorInsertmKikGR-V69T
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP2_1-395_V
Plasmid#147808PurposeMammalian Expression of HsDCP2_1-395DepositorInsertHsDCP2_1-395 (DCP2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT2-MEKK3-K392A
Plasmid#21585DepositorInsertmitogen-activated protein kinase kinase kinase 3 (MAP3K3 Human)
UseTagsExpressionMammalianMutationLysine 392 to ArgininePromoterAvailable sinceNov. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1371-sgITGB1-1
Plasmid#121533PurposesgITGB1-1 sequence: GAAGCAGGGCCAAATTGTGGG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.DepositorInsertsgITGB1-1
UseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromotermouse U6Available sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
MDH1-shDusp5-2-H2Kb2
Plasmid#17853DepositorInsertshDusp5 (DUSP5 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1371-sgITGB4-1
Plasmid#121535PurposesgITGB4-1 sequence: GAGGCGCAGTCCTTATCCACA. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.DepositorInsertsgITGB4-1
UseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromotermouse U6Available sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-PIWI(aa478-860)
Plasmid#21542DepositorInsertAgo2 (AGO2 Human)
UseTagsGSTExpressionMammalianMutationcontain only aa478-860PromoterAvailable sinceJuly 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
MDH1-miR-222-PGK-GFP-Blast
Plasmid#31522DepositorInsertmiR-222 (Mir222 Mouse)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(human)(R332P)
Plasmid#79037PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(human R332P) (TRIM5 Human)
UseTagsOneSTrEP-FLAGExpressionInsectMutationChanged Arg 332 to ProPromoterpolyhedrinAvailable sinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP104-ChromeQ-mCherry
Plasmid#123125PurposeAAV-mediated expression of ChromeQ-mCherry under the GFAP promoter.DepositorInsertChromeQ-mCherry
UseAAVTagsmCherryExpressionMammalianMutationChannelrhodopsin-2 (ChR2) with mutations A71S/ E9…PromoterGFAP104Available sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB222
Plasmid#68613PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB219
Plasmid#68610PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
SNX3-PX (1-162)
Plasmid#119084PurposeBacterial expression of human phox homology (PX) domain, SNX3-PX (1-162)DepositorInsertSNX3-PX (1-162) (SNX3 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRA-30-picky_sticky
Plasmid#138983PurposeIVT template for the alpha subunit of the mouse TCR #30 that is reactive against MC38DepositorInsertTCRA (Tcra Mouse)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRB-33-MC_hammer
Plasmid#138990PurposeIVT template for the beta subunit of the mouse TCR #33 that is reactive against MC38DepositorInsertTCRB (Tcrb Mouse)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRB-32-riot_punisher
Plasmid#138988PurposeIVT template for the beta subunit of the mouse TCR #32 that is reactive against MC38DepositorInsertTCRB (Tcrb Mouse)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRA-32-riot_punisher
Plasmid#138987PurposeIVT template for the alpha subunit of the mouse TCR #32 that is reactive against MC38DepositorInsertTCRA (Tcra Mouse)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRB-31-murderous_crow
Plasmid#138986PurposeIVT template for the beta subunit of the mouse TCR #31 that is reactive against MC38DepositorInsertTCRB (Tcrb Mouse)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRA-31-murderous_crow
Plasmid#138985PurposeIVT template for the alpha subunit of the mouse TCR #31 that is reactive against MC38DepositorInsertTCRA (Tcra Mouse)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRB-30-picky_sticky
Plasmid#138984PurposeIVT template for the beta subunit of the mouse TCR #30 that is reactive against MC38DepositorInsertTCRB (Tcrb Mouse)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-PIG-miR-497/miR-195
Plasmid#64236PurposeExpresses miR-497 and miR-195 in mammalian cellsDepositorUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMDC32-FIT2
Plasmid#96990PurposeExpress mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-Flag CASTOR1 I280A
Plasmid#84497PurposeLentiviral expression of CASTOR2 I280A mutantDepositorInsertCASTOR1 I280A (GATSL3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationI280APromotersestrin2Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
EC71139
Plasmid#154062PurposeLevel 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)DepositorInsertpZmUbi
UseSynthetic BiologyTagsExpressionMutationAll BsaI, Esp3I and BpiI restriction sites were r…PromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only