We narrowed to 13,118 results for: CRISPR-Cas9

1. pAC-CR7T-gRNA2.1-nlsBFP

   Plasmid

   #170515

   Purpose

   gRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR.

   Depositor

   Chun Han

   Article

   Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   CR7T

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PU6::klp-12_sgRNA

   Plasmid

   #46170

   Purpose

   klp-12 targeting sgRNA

   Depositor

   John Calarco

   Article

   Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

   Insert

   klp-12 targeting sgRNA (klp-12 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   C. elegans U6 snRNA pol III promoter

   Available since

   July 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAdSh.U6.gRNAGFP

   Plasmid

   #58255

   Purpose

   U6 promoter-driven single guide RNA complementary to eGFP

   Depositor

   Manuel Goncalves

   Article

   Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

   Insert

   U6.gRNAGFP cassette
   

   Use

   Adenoviral and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS SK mCherryROSAbsr

   Plasmid

   #54321

   Purpose

   Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.

   Depositor

   Silvestro Conticello

   Article

   Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

   Insert

   mCherry and BlasticidinS-resistance
   

   Use

   CRISPR, Mouse Targeting, and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgRNA1_Tet-inducible Luciferase reporter

   Plasmid

   #64161

   Purpose

   Photoactivatable transcription system. Mammalian expression of sgRNA1 to target Tet-inducible-luciferase reporter.

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA1 for Tet-inducible Luciferase reporter
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. sgRNA3_GAL4UAS-Luciferase reporter

   Plasmid

   #64159

   Purpose

   Photoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA3 for GAL4UAS-Luciferase reporter
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAdSh.U6.gRNAS1

   Plasmid

   #58252

   Purpose

   U6 promoter-driven single guide RNA complementary to the human AAVS1 locus

   Depositor

   Manuel Goncalves

   Article

   Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

   Insert

   U6.gRNAS1 cassette
   

   Use

   Adenoviral and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. U6-spTRE3G-CMV-mTagBFP2

   Plasmid

   #102854

   Purpose

   A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   sp-TRE3G-sgRNA; mTagBFP2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. sgRNA1_GAL4UAS-Luciferase reporter

   Plasmid

   #64157

   Purpose

   Photoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA1 for GAL4UAS-Luciferase reporter
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMG

    Plasmid

    #138264

    Purpose

    Yeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Inserts

    eGFP flanked by iCas9 sites
    mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tef1

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgRNA2_GAL4UAS-Luciferase reporter

    Plasmid

    #64158

    Purpose

    Photoactivatable transcription system. Lentiviral expression of sgRNA2 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA2 for GAL4UAS-Luciferase reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIK198

    Plasmid

    #65629

    Purpose

    "flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.

    Depositor

    Iskra Katic

    Article

    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC19-EAMP

    Plasmid

    #138265

    Purpose

    mCherry acceptor plasmid with iCas9 target site upstream of mCherry to be used in plasmid to plasmid integration reporter assay.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    iCas9 target-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-HTLV

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-hsg(NT:NT)

    Plasmid

    #138260

    Purpose

    Expression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    Nontargeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSB1C3-FeGFP

    Plasmid

    #138261

    Purpose

    Donor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmid

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    eGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    None

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. C113m

    Plasmid

    #121012

    Purpose

    MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    Cas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMLS280

    Plasmid

    #73738

    Purpose

    Simple cloning vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTHAP12-N1.1.2-gDNA

    Plasmid

    #175860

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    THAP12-Nickase2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCHCHD3-N2.1.2-gDNA

    Plasmid

    #175858

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    CHCHD3-Nickase2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCHCHD3-N1.1.1-gDNA

    Plasmid

    #175857

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    CHCHD3-Nickase1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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