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W118-1_eGFP

Plasmid

#180379

Purpose

lentiviral transduction of eGFP gene

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

eGFP (eGFP )

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hSTK16-Flag

Plasmid

#180403

Purpose

lentiviral transduction of human STK16 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

STK16 (STK16 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_Flag-hSRPK3

Plasmid

#180404

Purpose

lentiviral transduction of human SRPK3 (with an N-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

SRPK3 (SRPK3 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shTgm5-1

Plasmid

#180394

Purpose

Producing AAV that encodes mouse Tgm5 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shTgm5-1 (Tgm5 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shTgm5-2

Plasmid

#180395

Purpose

Producing AAV that encodes mouse Tgm5 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shTgm5-2 (Tgm5 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shTgm5-3

Plasmid

#180396

Purpose

Producing AAV that encodes mouse Tgm5 shRNA-3 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shTgm5-3 (Tgm5 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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HK13A Clone 13A light chain

Plasmid

#192182

Purpose

Clone 13A light chain (HK13A)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 13A light chain (HK13A)

Expression

Mammalian

Mutation

NA

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pKeratin-emiRFP2-N1

Plasmid

#178972

Purpose

Expresses the fusion protein of Keratin-emiRFP2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

Keratin-emiRFP2

Expression

Mammalian

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW-shRIIα

Plasmid

#183454

Purpose

sgRNA targeting rat PKA-RIIα subunits

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA targeting rat PKA-RIIα (Prkar2a Rat)

Use

Lentiviral

Promoter

shRNA: H1 / gene: ubiquitin

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW-scrambled

Plasmid

#183455

Purpose

This control vector contains a scrambled version of the targeting sequence used in the pFUGW-shRIIα construct

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

scrambled sgRNA

Use

Lentiviral

Promoter

shRNA: H1 / gene: ubiquitin

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.mTagBFP2.miR-125b-2

Plasmid

#169311

Purpose

Constitutive overexpression of miR-125b-2 with mTagBFP2 as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-125b-2 (MIR125B2 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pIRES-EGFP-TPD54

Plasmid

#172459

Purpose

Expression of untagged Tumor Protein D52 like 2 (TPD54/TPD52L2) and GFP

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD54 (TPD52L2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO ACD11

Plasmid

#170738

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Cell Rep. 2014 Jan 30;6(2):388-99. doi:

Insert

acd11 (ACD11 Mustard Weed)

Tags

SUMO

Expression

Bacterial

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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AIDx-ID-208

Plasmid

#135345

Purpose

Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 208 of nSpyCas9

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx-ID-208

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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AIDx-ID-468

Plasmid

#135346

Purpose

Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 468 of nSpyCas9

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx-ID-468

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Orf6

Plasmid

#157726

Purpose

mammalian expression of untagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Orf6 (ORF6 SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp15

Plasmid

#157720

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp15 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp14

Plasmid

#157717

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp14 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMS22

Plasmid

#129710

Purpose

Expresses Escherichia coli RssB E134G:E135S:E136G:E137S variant as an N-terminal 10His fusion

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

Escherichia coli RssB-GSGS variant (rssB Escherichia coli MG1655)

Tags

10-Histidine tag cleavable with PreScission Prote…

Expression

Bacterial

Mutation

RssB E134G:E135S:E136G:E137S

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre;3xP3-gTc’v-SV40]

Plasmid

#124068

Purpose

CRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony for linearization; (Transformation plasmid; Black eye marker)

Depositor

Gregor Bucher

Article

He et al Elife. 2019 Oct 18;8. pii: 49065. doi: 1

Insert

eb-Tc’bhsp-EGFP-2A-Cre; 3xP3-gTc’v-SV40

Use

CRISPR and Cre/Lox

Expression

Insect

Promoter

bhsp68

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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