We narrowed to 7,454 results for: ski

1. pLX307 MCL1

   Plasmid

   #117726

   Purpose

   Open reading frame vector encoding MCL1

   Depositor

   William Hahn

   Article

   Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

   Insert

   MCL1L (MCL1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1-TRC.mKO2_shGFP

   Plasmid

   #110318

   Purpose

   Control (Target CAAGCTGACCCTGAAGTTCAT), silence GFP and express monomeric Kusabira-Orange2

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   GFP
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 SIRT7_H187Y

   Plasmid

   #53151

   Purpose

   expression vector for catalytically inactive human Sirtuin7

   Depositor

   Katrin Chua

   Article

   Shin et al Cell Rep. 2013 Nov 14;5(3):654-65. doi:

   Insert

   Sirt7 (SIRT7 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   H187Y, catalytic inactive

   Promoter

   CMV

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. MAC-FURIN

   Plasmid

   #158386

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   furin, paired basic amino acid cleaving enzyme (FURIN Human)
   

   Use

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pscAAV-CAG-RLuc

   Plasmid

   #83280

   Purpose

   recombinant AAV vector packaging self-complementary Renilla Luciferase under the CAG promoter

   Depositor

   Mark Kay

   Article

   Paulk et al Mol Ther Methods Clin Dev. 2018 Jul 24;1

   Insert

   Renilla Luciferase
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSH-CAG-AtAFB2.F74A-IRES-puro

   Plasmid

   #216245

   Purpose

   Express AtAFB2.F74A mutant without tag for AID2

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   AtAFB2.F74A
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   Expression

   Mammalian

   Mutation

   F74A mutation

   Promoter

   CAG

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. MCP-MSN

   Plasmid

   #210704

   Purpose

   This plasmid express EF1a promoter driven MCP-MSN transactivation module

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   MCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N55K in MCP

   Promoter

   EF1a

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA6-N-Flag-SREBP1

   Plasmid

   #134283

   Purpose

   Expresses Flag-tagged SREBP1 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   SREBP1 (Srebf1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   mouse SREBP1 isoform a precursor

   Promoter

   CMV

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-actin prom-dsRed-adra2a.1227.shRNA

   Plasmid

   #67880

   Purpose

   expresses dsRed and shRNA to knock down adra2a receptors

   Depositor

   William Wisden

   Article

   Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

   Insert

   dsRed and shRNA to knock down the mouse adra2 receptor
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chicken beta actin

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pIZ-EGFP-MYO10-HMM

    Plasmid

    #87257

    Purpose

    Expression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYO10 (Myo10 Mouse)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Myo10 is truncated to contain aa.1-941

    Promoter

    OpIE2

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX4T3-HA-ERK2 GOF

    Plasmid

    #53200

    Purpose

    Expresses GST and HA tagged human ERK2 R67S D321N from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    ERK2 (MAPK1 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    R67S/D321N

    Promoter

    Available since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

12. FokI-mCherry-LacI-NLS

    Plasmid

    #215096

    Purpose

    Expresses FokI-mCherry-LacI-NLS in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    FokI-mCherry-LacI-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe H-Ras 12V

    Plasmid

    #12545

    Depositor

    Channing Der

    Article

    Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

    Insert

    H-Ras 12V (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    12V constituitively activated.

    Promoter

    Available since

    Sept. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-PTRE-tight-flex-hM3Dq-mCherry-WPRE-pA

    Plasmid

    #115161

    Purpose

    This plasmid is for use with neuronal cell-type selective activity tagging. The hM3Dq receptor expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Harding et al Curr Biol. 2018 Jul 23;28(14):2263-2273.

    Inserts

    PTRE - tet activator responsive promoter
    flex sequence
    hM3dq-mCherry (inverted)
    flex sequence
    

    Use

    AAV

    Tags

    hM3Dq-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFRT-FLAG-HA-(N)-CDK12

    Plasmid

    #231750

    Purpose

    Expression in Flp-IN human cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    CDK12 (CDK12 Human)
    

    Use

    Tags

    Flag-HA-3C

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. MCP-eN3x9

    Plasmid

    #210706

    Purpose

    This plasmid express EF1a promoter driven MCP-eN3x9 transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MCP fused to transactivation domain comprising eNRF2 and 3x 9aa Transactivation domain
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    EF1a

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV2-CAG-mGreenLantern-KASH

    Plasmid

    #191094

    Purpose

    To express a bright monomeric green FP to label the eucaryotic nuclear envelope. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Pantelis Tsoulfas, Murray Blackmore

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    mGreenLantern-KASH
    

    Use

    AAV and Synthetic Biology

    Tags

    mGreenLantern fused to the KASH domain of Nesprin…

    Expression

    Mammalian

    Mutation

    Optimizzation was done using the Human codon usage

    Promoter

    CMV enhancer and CAG

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.CAG.SF-iGluSnFR.A184S

    Plasmid

    #106198

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Tags

    Expression

    Mutation

    GltI: A184S

    Promoter

    CAG

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40

    Plasmid

    #101064

    Purpose

    AAV vector expressing CaMPARI2_L398T (Kd = 825nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Has Service

    AAV1

    Insert

    CaMPARI2_L398T
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Expression

    Mutation

    Promoter

    hsyn (synapsin-1)

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBabe N-Ras 61K

    Plasmid

    #12543

    Depositor

    Channing Der

    Article

    Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

    Insert

    NRas 61K (NRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    61K constitutively activated.

    Promoter

    Available since

    Sept. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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