We narrowed to 14,335 results for: cas9

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pX330-Puro-ccdB

Plasmid

#82580

Purpose

A modified version of plasmid pX330 (#42230): Added are a ccdB stuffer and a Puromycin selection cassette

Depositor

Ivo Huijbers

Article

Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

CBh

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAS_sfGFP150K

Plasmid

#193313

Purpose

pAS control plasmid for sfGFP expression

Depositor

Simon Elsaesser

Article

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Insert

super folder GFP

Expression

Mammalian

Mutation

150K in sfGFP

Promoter

EF1

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAP973-1

Plasmid

#99496

Purpose

TEV::linker::meGFP:3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::linker::meGFP::3XFlag

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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EC70133

Plasmid

#209507

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

Cas12a-tRNA-DR-DR

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70134

Plasmid

#209508

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

Cas12a-tRNA-DR-DR

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMLS252

Plasmid

#73720

Purpose

SapTrap GFP + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS291

Plasmid

#73724

Purpose

SapTrap mCherry + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

mCherry + Cbr-unc-119

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMZ283

Plasmid

#52224

Purpose

Expression of sgRNA precursor with CspCI placeholder for target cloning (see comments).

Depositor

Mikel Zaratiegui

Article

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Insert

rrk1-sgRNA

Use

CRISPR

Expression

Yeast

Promoter

rrk1

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pMLS286

Plasmid

#73723

Purpose

Saptrap tagRFP + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

tagRFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pWD509

Plasmid

#73739

Purpose

Gateway compatible [1-2] Entry Vector encoding 2xNLS-FLP-D5

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

2xNLS-FLP-D5

Expression

Worm

Mutation

G5D

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pH1v1

Plasmid

#60244

Purpose

gRNA expression by the H1 promoter

Depositor

Donald Zack

Article

Ranganathan et al Nat Commun. 2014 Aug 8;5:4516. doi: 10.1

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

H1 promoter

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pAP974-1

Plasmid

#99497

Purpose

TEV::linker::tagRFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::linker::tagRFP::3XFlag

Expression

Bacterial

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBLO5.01

Plasmid

#121418

Purpose

pUC19_BsaI-dCas9-BsaI

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

dcas9

Expression

Bacterial

Promoter

lac

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pKER-mCerulean

Plasmid

#86622

Purpose

Expresses mCerulean from the EF1a promoter in mammalian cells

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

mCerulean

Expression

Mammalian

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pDD377

Plasmid

#91823

Purpose

Codon-optimized mTurquoise2 donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

mTurquoise2-C1

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB8739

Plasmid

#126905

Purpose

Template for PCR amplification of XdCrtYB

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtYB

Use

Synthetic Biology

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMZ284

Plasmid

#52225

Purpose

Expression of sgRNA precursor targeted against ade6+

Depositor

Mikel Zaratiegui

Article

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Insert

rrk1-sgRNA

Use

CRISPR

Expression

Yeast

Promoter

rrk1

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pAP997-1

Plasmid

#99500

Purpose

3XFlag::tagRFP::linker::TEV

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::tagRFP::linker::TEV

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB8740

Plasmid

#126906

Purpose

Template for PCR amplification of XdCrtI

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI

Use

Synthetic Biology

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB8741

Plasmid

#126907

Purpose

Template for PCR amplification of XdCrtE

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtE

Use

Synthetic Biology

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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