We narrowed to 10,665 results for: t7

1. pT7-L1T1L-NLuc

   Plasmid

   #128942

   Purpose

   Expresses L1-NLuc gene

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al J Virol. 2019 May 8. pii: JVI.00401-19.

   Insert

   L1 minor core protein
   

   Tags

   Nanoluc

   Expression

   Bacterial

   Promoter

   T7 promotor

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET51b(+)-SNAP-CLIP

   Plasmid

   #127180

   Purpose

   Bacterial expression of the fused self-labeling tags SNAP-tag and CLIP-tag

   Depositor

   Kai Johnsson

   Article

   Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

   Insert

   SNAP-CLIP
   

   Tags

   HisTag and StrepTag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET26b_Nb.AT110.i1

   Plasmid

   #135481

   Purpose

   E. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptor

   Depositor

   Andrew Kruse

   Article

   Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

   Insert

   Nanobody AT110.i1
   

   Tags

   6xHis-tag and pelB leader

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET19b+H2B(T116C)

   Plasmid

   #157808

   Purpose

   Expression of human histone H2B with T116C mutation to facilitate maleimide chemistry

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   H2B(T116C)
   

   Expression

   Bacterial

   Mutation

   T116C

   Promoter

   T7

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRAF-MutL

   Plasmid

   #86607

   Purpose

   E. coli MutL expression plasmid

   Depositor

   Richard Fishel

   Article

   Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

   Insert

   E. coli MutL
   

   Tags

   his-tag and ald-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTEV5_ RpMatB_1

   Plasmid

   #78205

   Purpose

   Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor

   Depositor

   Martin Webb

   Article

   Vancraenenbroeck et al ACS Chem Biol. 2015 Nov 20;10(11):2650-7

   Insert

   matB (His6 C106A R286C Q457C K488A)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   C106A R286C Q457C K488A

   Promoter

   T7

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pCellFree_G03 TFEC

   Plasmid

   #67060

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   TFEC (TFEC Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pET21a-hAIM2-His6

   Plasmid

   #51532

   Purpose

   Express His6-tagged hAIM2 in bacteria

   Depositor

   Emad Alnemri

   Article

   Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

   Insert

   AIM2 (AIM2 Human)
   

   Tags

   His6 and T7

   Expression

   Bacterial

   Promoter

   lac operator

   Available Since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. SpyCatcher002-oHept

   Plasmid

   #124671

   Purpose

   Expresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coil

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyCatcher002-oHept
   

   Tags

   C-tag, His6, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. 2xNLS-pMJ915v2

    Plasmid

    #88916

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCellFree_G03 WAVE 2

    Plasmid

    #67098

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    WAVE 2 (WASF2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRAF-MutH

    Plasmid

    #86609

    Purpose

    E. coli MutH expression plasmid

    Depositor

    Richard Fishel

    Article

    Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

    Insert

    E. coli MutH
    

    Tags

    his-tag and ald-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRAF-MutS

    Plasmid

    #86605

    Purpose

    E. coli MutS expression plasmid

    Depositor

    Richard Fishel

    Article

    Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

    Insert

    E. coli MutS (D835R,R840E)
    

    Tags

    his-tag and ald-tag

    Expression

    Bacterial

    Mutation

    D835R, R840E

    Promoter

    T7

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET32-sLatherin

    Plasmid

    #61685

    Purpose

    bacterial expression of the horse sweat/saliva protein Latherin as a His-tagged thioredoxin fusion

    Depositor

    Brian Smith

    Article

    Vance et al J R Soc Interface. 2013 Jun 19;10(85):20

    Insert

    Latherin (LATH horse)
    

    Tags

    HIS and Trx-Tag thioredoxin protein

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

15. EcMBP165-cpGFP.PPYF.T203V.pRSET

    Plasmid

    #33373

    Purpose

    a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

    Depositor

    Loren Looger

    Article

    Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

    Insert

    Improved MBP based Maltose Biosensor
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    cpGFP146 variant from GCaMP2 inserted between AA1…

    Promoter

    T7

    Available Since

    Dec. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-GR-AKAR3

    Plasmid

    #173015

    Purpose

    Improved green-red FRET-based PKA activity reporter.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    GR-AKAR3
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. His6_BcLOV4_mCherry_BamUK

    Plasmid

    #114596

    Purpose

    Encodes mCherry-tagged BcLOV4 for expression in bacterial cells

    Depositor

    Brian Chow

    Article

    Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

    Insert

    BcLOV4
    

    Tags

    His6 and mCherry

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET26b_Nb.AT110

    Plasmid

    #135480

    Purpose

    E. coli periplasmic expression of conformational nanobody Nb.AT110 recognizing angiotensin II type 1 receptor

    Depositor

    Andrew Kruse

    Article

    Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

    Insert

    Nanobody AT110
    

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWaldo-GFPd_PepTSt

    Plasmid

    #58333

    Purpose

    Expresses the POT family transporter from Streptococcus thermophilus in E. coli as a C-terminally tagged GFP his tagged protein

    Depositor

    Simon Newstead

    Article

    Solcan et al EMBO J. 2012 Aug 15;31(16):3411-21. doi:

    Insert

    PepTSt
    

    Tags

    GFP-His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

20. VV001: WT Arch-3 in pET28b

    Plasmid

    #58487

    Purpose

    Expresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coli

    Depositor

    Adam Cohen

    Article

    Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

    Insert

    Archaerhodopsin-3 (with C-terminal His tag)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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