We narrowed to 22,280 results for: fus

1. pCMV-AAAAA-nCas9(D10A)-UGI

   Plasmid

   #187409

   Purpose

   DddA AAAAA variant

   Depositor

   Jin-Soo Kim

   Article

   Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

   Insert

   DddA AAAAA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+) prME (JEV) sdm2

   Plasmid

   #52881

   Purpose

   expression of Japanese Encephalitis Virus VLPs in mammalian cells

   Depositor

   Yorgo Modis

   Article

   Nour et al PLoS Pathog. 2013;9(9):e1003585. doi: 10

   Insert

   prME (POLY Japanese encephalitis virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   sdm2

   Promoter

   CMV

   Available since

   May 14, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP

   Plasmid

   #213119

   Purpose

   Expression of an ActA FKBP payload for MinDE recruitment circuits in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   ActA(1-584)-BFP-FKBP (actA Listeria monocytogenes)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB-dCas9-PBE

   Plasmid

   #98159

   Purpose

   Expresses CRISPR base editor in maize

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

   Insert

   NLS-APOBEC1-XTEN-dCas9-UGI-NLS
   

   Use

   Maize

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Ubi

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLC-ZsGreen-P2A-Hygro

   Plasmid

   #124301

   Purpose

   Lentiviral vector for expression of ZsGreen-P2A-Hygro casette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   ZsGreen
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. CROP_C9_Puro_DNMT3B

   Plasmid

   #183284

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets DNMT3B gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   DNMT3B
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Aurli_150841_GZG

   Plasmid

   #162563

   Purpose

   Inactivation of Aurli_150841 (crtIBY) in Aurantiochytrium limacinum MYA-1381 with zeocin resistance driven by native GAPDH promoter/termiator system.

   Depositor

   Jackie Collier

   Article

   Rius et al Genome Biol Evol. 2023 Mar 3;15(3):evad0

   Insert

   shble
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   GAPDH

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJL-SaBE3

   Plasmid

   #85169

   Purpose

   Expresses SaBE3 in mammalian cells

   Depositor

   David Liu

   Article

   Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   SaBE3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLC-HA-Nedd8-P2A-Hygro

   Plasmid

   #124306

   Purpose

   Lentiviral vector for expression of HA tagged Nedd8-P2A-Hygro casette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   Nedd8 (NEDD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-Vti1b

    Plasmid

    #45914

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    vesicle transport through interaction with t-SNAREs 1B (VTI1B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

11. Cenp-B DBD INCENP GFP

    Plasmid

    #45237

    Purpose

    Target INCENP to centromeres via CENP-B DNA binding domain (DBD)

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    Cenp-B-DBD, INCENP-Δcen, GFP
    

    Use

    Tags

    DNA binding domain of CENP-B and GFP

    Expression

    Mammalian

    Mutation

    CEN box deleted from INCENP (aa1-46)

    Promoter

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti X2 Zeo DEST (749-3)

    Plasmid

    #21562

    Purpose

    3rd gen lentiviral Gateway destination vector, RNAi, 3'LTR insertion, Zeo

    Depositor

    Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-mCherry-2xEcMTS

    Plasmid

    #133624

    Purpose

    Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    mCherry-2xEcMTS
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFSW Syt1 WT IRES GFP

    Plasmid

    #133816

    Purpose

    Encodes full-length wild type synaptotagmin 1 for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRXIP GFP-VAMP8

    Plasmid

    #45919

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    vesicle-associated membrane protein 8 (VAMP8 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

16. p35S-A3A-PBE

    Plasmid

    #119773

    Purpose

    Conversion of C to T in potato

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS-UGI-NLS
    

    Use

    Tags

    NLS (flanking UGI)

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    CaMv 2x35S

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C

    Plasmid

    #61966

    Purpose

    Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))

    Depositor

    Lynne Regan

    Article

    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

    Inserts

    N-mCherry-ME(pS)VD
    TRAP(RK)-C-mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD and PLtetO

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMRXIP GFP-VAMP7

    Plasmid

    #45920

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    vesicle-associated membrane protein 7 (Vamp7 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pXint129

    Plasmid

    #178208

    Purpose

    Encodes bacteriophage lamda excisionase and integrase to facilitate in vivo Gateway LR clonase reaction.

    Depositor

    Gregory Phillips

    Article

    Platt et al Plasmid. 2000 Jan . 43(1):12-23.

    Insert

    xis excisionase, int integrase (xis, lambdap32 Bacteriophage lambda)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacI

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMD1 anti-GFP nanobody-Tn5

    Plasmid

    #215567

    Purpose

    Expresses anti-GFP nanobody-Tn5 fusion in bacteria with an N-terminal 3xFlag tag and a C-terminal intein-CBD-intein tag

    Depositor

    Isabelle Mansuy

    Article

    Maria A. et al bioRxiv 2024.07.12.603224

    Insert

    anti-GFP nanobody-Tn5 fusion
    

    Use

    Tags

    3xFlag and intein-CBD

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CROP_C9_Puro_DNMT3A

    Plasmid

    #183283

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets DNMT3A gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    DNMT3A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHJ8: CadC-VHH-Control

    Plasmid

    #108243

    Purpose

    membrane bound transcription activator CadC fusing with anti-RNase A antibody

    Depositor

    Jerome Bonnet

    Article

    Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

    Insert

    Membrane bound transcription activator CadC fusing with anti-RNase A VHH antibody
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pLacO1

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEF-ENTR-LacZ (710-1)

    Plasmid

    #17430

    Purpose

    Gateway entry vector with EF1-a promoter, V5-His C-term, Lac Z

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    LacZ
    

    Use

    Entry vector

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA-ALFA-tag-Gal4-VP16

    Plasmid

    #199465

    Purpose

    Expression of the Gal4-ALFA-tag-VP16 synthetic transcription factor containing the ALFA-tag sequence in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

    Insert

    Gal4-ALFA-tag-VP16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti CMV/TO V5-LUC Puro (w549-1)

    Plasmid

    #19785

    Purpose

    3rd gen lentiviral V5-Luciferase expression vector, CMV/TO promoter, Puro

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AAV-H2GH

    Plasmid

    #182202

    Purpose

    Adeno-associated viral vector to express histone2B-HaloTag-GFP fusion, a nuclear-localized Halotag-GFP fusion construct

    Depositor

    Joshua Kritzer

    Article

    Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

    Insert

    histone2B-HaloTag-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CROP_C9_Puro_PARP1

    Plasmid

    #183312

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets PARP1 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    PARP1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GFP-V96 ELP

    Plasmid

    #67853

    Purpose

    GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

    Depositor

    Andrew MacKay

    Article

    Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

    Insert

    GFP fused to ELP (VPGVG) repeated 96 times
    

    Use

    Tags

    GFP fused to N-terminal of ELP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PAmCherry1-N1-PHPLCd1

    Plasmid

    #202679

    Purpose

    Photoactivatable lipid biosensor

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    PLCD1v2(1-170):PAmCherry1 (PLCD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CROP_C9_Puro_MYC

    Plasmid

    #183330

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets MYC gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    MYC
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge