We narrowed to 22,191 results for: his
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pTorPE-G-GECO1
Plasmid#32466DepositorInsertG-GECO1.0
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFQAQLGGL…ExpressionBacterialMutationGCaMP3 K119I/L173Q/S404G/E430VPromoterAvailable SinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
Cathepsin S S21C/C25A
Plasmid#84695Purposefor expression of mutant Cathepsin S in Leishmania tarantolaeDepositorInsertcathepsin S (CTSS Human)
UseExpression in leishmania tarantolaeTagshis-tagExpressionMutationS21C, C25APromoterunknownAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAW-AlphaLobe
Plasmid#113255PurposeE. coli expression vector for His-MBP-Cas9 Alpha-Helical LobeDepositorInsertS. pyogenes Cas9 alpha-helical lobe
UseTags10xHis-MBPExpressionBacterialMutationresidues 56–714; See paper for descriptionPromoterT7Available SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
Mfn1(K88T)-10xmyc
Plasmid#26050DepositorAvailable SinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pcmvtag2Bmciap2
Plasmid#11463DepositorAvailable SinceMarch 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP8 (full length))
Plasmid#16361DepositorInserthuman SENP8 full length (SENP8 Human)
UseTagsHisExpressionBacterialMutationhuman SENP8 full length, see Depositor CommentsPromoterAvailable SinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-NEMO
Plasmid#11966DepositorAvailable SinceJuly 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
Caspase-6
Plasmid#100102PurposeBacterial expression of Caspase-6DepositorAvailable SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R196M wgMDH
Plasmid#204273PurposeBacterial expression of R196M wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR196MPromoterAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 D193E wgMDH
Plasmid#204269PurposeBacterial expression of D193E wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationD193EPromoterAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEW3: pET-32a Dot1L(2-416)
Plasmid#124098PurposeDot1L(2-416) expression plasmidDepositorAvailable SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEMI91
Plasmid#74888PurposeShuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more informationDepositorInsertTitin I27 x 9 (TTN Human)
UseTagsHis-tag and Strep-tag IIExpressionBacterialMutationPromoterT7 promoterAvailable SinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21c(+)-EFG1mt
Plasmid#31076DepositorInsertMature form human mitochondrial elongation factor G isoform 1 (GFM1 Human)
UseTagsHisExpressionBacterialMutationIncludes the coding sequence for the mature form.…PromoterAvailable SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-PSMD10
Plasmid#31332DepositorInsertPSMD10, proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (gankyrin) (PSMD10 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET21c(+)-mtPheRS
Plasmid#31080DepositorInsertMature form human mitochondrial phenylalanine-tRNA synthetase (FARS2 Human)
UseTagsHisExpressionBacterialMutationIncludes coding sequence for the mature form. Nt …PromoterT7Available SinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLentiEKAR2G2
Plasmid#40178DepositorInsertErk activity reporter
UseLentiviralTagsHis-MycExpressionMammalianMutationPromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-YWHAE
Plasmid#31562DepositorInserttyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfGCaMP6s
Plasmid#100022PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.DepositorInsertsfGCaMP6s
UseTags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFPPromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-H3.3-YFP
Plasmid#8693DepositorAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLentiEKAR2G1
Plasmid#40177DepositorInsertErk activity reporter
UseLentiviralTagsHis-MycExpressionMammalianMutationPromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
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A3Bmax
Plasmid#207167PurposeExpress full-length human A3B in a BE4max-based base editorDepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)
UseTagsNLS and NLS, 6x HisExpressionMammalianMutationPromoterCMVAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pET-PLS-KP6_rpoA-U
Plasmid#190967PurposeTest the U-to-C editing activity of KP6 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
UseTagsHisExpressionBacterialMutationPromoterT7Available SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
WNK2 K207A
Plasmid#24570DepositorAvailable SinceJune 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
p5077 pcDNA4c hBrd4 (1-594)
Plasmid#14453DepositorInsertBrd4 (1-594) (BRD4 Human)
UseTagsHisExpressionMammalianMutationamino acids 1-594PromoterAvailable SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 BATF3
Plasmid#67050PurposeStandard for cell-free expression benchmarking.DepositorInsertBATF3 (BATF3 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-Claspin-FL-wt
Plasmid#31621DepositorAvailable SinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDEST- IP2.0(codon for C.elegans)
Plasmid#109216PurposeIP2.0 for C. elegans (Codon optimization and introns).DepositorInsertIP2.0 (Inverse Pericam 2.0)
UseTagsHis-tagExpressionWormMutationPromoterAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
NpT7-5-Erk2
Plasmid#39230DepositorAvailable SinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
HM3C NaVAe1_p pET24b
Plasmid#127854PurposeBacterial expression vector for NaVAe1 bacterial sodium channel residues 143-288. It expresses HIStag-MBP-3C-channel protein.DepositorInsertNaVAe1p
UseTags3C Cleavage site, His, and MBPExpressionBacterialMutationPromoterAvailable SinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-GYG
Plasmid#31324DepositorAvailable SinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-36α
Plasmid#214343PurposeProtein overexpression in bacteriaDepositorAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TGFB1
Plasmid#67080PurposeStandard for cell-free expression benchmarking.DepositorInsertTGFB1 (TGFB1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern-plasma membrane/pcDNA3
Plasmid#51975Purposemammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membraneDepositorInsertNano-lantern
UseTagsHIS and LynExpressionMammalianMutationS257G in Rluc8PromoterAvailable SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
K401-iLid
Plasmid#122484PurposeComponent of optically-induced motor dimerization systemDepositorInsertKinesin 1-401 iLid His tag
UseTagsHis6 and iLidExpressionBacterialMutationKinesin amino acids 1-401PromoterT7Available SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKPY197
Plasmid#62599PurposeEncodes Thr412Gly-CePheRS Alpha SubunitDepositorInsertThr412Gly-CePheRS Alpha Subunit
UseTagsHISExpressionWormMutationThr412GlyPromoterAvailable SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-ntH6-eGFP
Plasmid#32557DepositorInserteGFP (eGFP )
UseSynthetic BiologyTags6 His tag with Thrombin siteExpressionBacterialMutationPromoterConsitutive lacAvailable SinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only
