We narrowed to 7,294 results for: 11

1. lentiCRISPR v2-Nr4a1sgRNA1

   Plasmid

   #160945

   Purpose

   Guide RNA 1 to generate Nr4a1 knockout by CRISPR

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Nr4a1 gRNA 1 (Nr4a1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)

   Plasmid

   #114090

   Purpose

   CAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized MbCas12a
   

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. psiCHECK2-E2F2 3'UTR

   Plasmid

   #29469

   Depositor

   Judy Lieberman

   Article

   Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

   Insert

   E2F2 3'UTR (E2F2 Human)
   

   Expression

   Mammalian

   Available Since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pEGFP-N3/hArf5(WT)-EGFP

   Plasmid

   #79534

   Purpose

   Expresses C-terminally EGFP-tagged Arf5(WT) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

   Insert

   Arf5 (ARF5 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMSCVpuro-F-Gcn5-NLSm

   Plasmid

   #63708

   Purpose

   retroviral expression of cytoplasm-localized mouse Gcn5 in mammalian cells

   Depositor

   Kai Ge

   Article

   Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

   Insert

   Gcn5 (Kat2a Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   mutated K425, K427 and K438 to R (arginine)

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pEGFP-RNF138-delta 86-105

   Plasmid

   #78922

   Purpose

   Mammalian expression of RNF138 with delta 86-105 and an EGFP fusion

   Depositor

   Michael Hendzel

   Article

   Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

   Insert

   RNF138 (RNF138 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   delta 86-105

   Promoter

   CMV

   Available Since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pEGFP-RNF138-delta 158-180

   Plasmid

   #78923

   Purpose

   Mammalian expression of RNF138 with delta 158-180 and an EGFP fusion

   Depositor

   Michael Hendzel

   Article

   Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

   Insert

   RNF138 (RNF138 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   delta 159-180

   Promoter

   CMV

   Available Since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pEGFP-RNF138-delta 189-215

   Plasmid

   #78924

   Purpose

   Mammalian expression of RNF138 with delta 189-215 and an EGFP fusion

   Depositor

   Michael Hendzel

   Article

   Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

   Insert

   RNF138 (RNF138 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   delta 189-215

   Promoter

   CMV

   Available Since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pNIA-CEN-mVenus-FLAG-LANS1-Set2

   Plasmid

   #122003

   Purpose

   mVenus-FLAG-LANS-Set2: mVenus- and FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast

   Depositor

   Brian Kuhlman

   Article

   Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d

   Insert

   mVenus-FLAG-LANS1-Set2 (SET2 Budding Yeast)
   

   Use

   Synthetic Biology

   Tags

   mVenus-FLAG

   Expression

   Yeast

   Mutation

   Set2 point mutations K538G, K539S, R549G, K550S, …

   Promoter

   ADH1

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Flag-M4-AR-W741C-S81A

    Plasmid

    #171248

    Purpose

    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala (AR-W741C-S81A)

    Depositor

    Steven Balk

    Article

    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

    Insert

    3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala mutant (AR Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-Trp741Cys-Ser81Ala (AR-W741C-S81A)

    Promoter

    CMV

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Flag-M4-AR-W741C-S81D

    Plasmid

    #171249

    Purpose

    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Asp (AR-W741C-S81D)

    Depositor

    Steven Balk

    Article

    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

    Insert

    3xFlag-tagged human androgen receptor Trp741Cys-Ser81Asp mutant (AR Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-Trp741Cys-Ser81Asp (AR-W741C-S81D)

    Promoter

    CMV

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-FLAG-IRES-blasticidin-Sox2 S248A

    Plasmid

    #182047

    Purpose

    expression of Sox2 mutants

    Depositor

    Hyonchol Jang

    Article

    Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

    Insert

    Sox2 (Sox2 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S248A

    Promoter

    CAG

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-FLAG-IRES-blasticidin-Sox2 T258A

    Plasmid

    #182048

    Purpose

    expression of Sox2 mutants

    Depositor

    Hyonchol Jang

    Article

    Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

    Insert

    Sox2 (Sox2 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T258A

    Promoter

    CAG

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-HtrA2-FLAG S142D

    Plasmid

    #15939

    Depositor

    L. Miguel Martins

    Article

    Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

    Insert

    HtrA2 (HTRA2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S142D

    Available Since

    Nov. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pLenti-Puro-sgMARK4

    Plasmid

    #138691

    Purpose

    Expresses a human MARK4-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgMARK4 human (MARK4 Human)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MAC-CLEC4D

    Plasmid

    #172411

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

    Insert

    C-type lectin domain family 4 member D (CD antigen CD368) (CLEC4D Human)
    

    Tags

    MAC-tag

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Nov. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pGL3-DICER-Prom Ebox double mut

    Plasmid

    #25854

    Depositor

    David E. Fisher

    Article

    Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

    Insert

    DICER-Promoter Ebox double mut (DICER1 Human, gene ID 23405)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-6P-1 p37 SHPmut

    Plasmid

    #113503

    Purpose

    Bacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP box

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Mutation

    F215A/E218A/QKL220-222AAA

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL3-DICER-Prom upstream Ebox Mut

    Plasmid

    #25852

    Depositor

    David E. Fisher

    Article

    Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

    Insert

    DICER-Promoter upstream Ebox Mut (DICER1 Human, gene ID 23405)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-DICER-Prom downstream Ebox Mut

    Plasmid

    #25853

    Depositor

    David E. Fisher

    Article

    Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

    Insert

    DICER-Prom downstream Ebox Mut (DICER1 Human, gene ID 23405)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge