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pMitoLOC

Plasmid

#58980

Purpose

Expression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrila

Depositor

Markus Ralser

Article

Vowinckel et al Mitochondrion. 2015 Sep;24:77-86. doi: 1

Inserts

mCherry
GFP

Tags

preCOX4 and preSU9

Expression

Yeast

Promoter

ADH1 and MET17

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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p8.91

Plasmid

#187441

Purpose

Lentiviral packaging plasmid expressing gag and pol.

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Inserts

Use

Lentiviral

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMDG

Plasmid

#187440

Purpose

VSV-G envelope expressing plasmid

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Inserts

Expression

Mammalian

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1a-IRES-Neo

Plasmid

#28019

Depositor

Thomas Zwaka

Article

Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

Type

Empty backbone

Expression

Mammalian

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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Golgi-mEGFP

Plasmid

#182877

Purpose

Localization: Gal-T - Golgi Complex

Depositor

Salvatore Chiantia

Article

Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

Insert

Golgi-mEGFP

Expression

Mammalian

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR-221-P3-P4

Plasmid

#186351

Purpose

Gateway donor vector with AttP3 AttP4 recombination sites

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway donor vector

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-KDEL

Plasmid

#226568

Purpose

Encodes mNeonGreen with KDEL ER lumenal target sequence

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

KDEL (ER lumenal target sequence)

Tags

mNeonGreen

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNX32-Dest

Plasmid

#105083

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Depositor

Christopher Grefen

Article

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Tags

NubG

Expression

Yeast

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pXN22-Dest

Plasmid

#105085

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Depositor

Christopher Grefen

Article

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Tags

NubG-3xHA

Expression

Yeast

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pHJ322

Plasmid

#20677

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Moesin (MSN Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Thr 558 to Asp

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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hBax C3-EGFP

Plasmid

#19741

Depositor

Richard Youle

Article

Nechushtan et al EMBO J. 1999 May 4. 18(9):2330-41.

Insert

Bax (BAX Human)

Tags

GFP

Expression

Mammalian

Mutation

none

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pBN41

Plasmid

#86716

Purpose

C. elegans injection marker

Depositor

Peter Askjaer

Article

Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

Insert

Pmyo-2::GFP

Expression

Worm

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-SMN

Plasmid

#37057

Depositor

Greg Matera

Article

Shpargel et al Proc Natl Acad Sci U S A. 2005 Nov 29;10

Insert

Human SMN1 (SMN1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pXN21-Dest

Plasmid

#105086

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Depositor

Christopher Grefen

Article

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Tags

NubG

Expression

Yeast

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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2C::tdTomato Reporter

Plasmid

#40281

Depositor

Samuel Pfaff

Article

Macfarlan et al Nature. 2012 Jul 5;487(7405):57-63.

Insert

2C Promoter (2-cell stage promoter)

Tags

tdTomato

Expression

Mammalian

Available Since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pSHARK2

Plasmid

#248168

Purpose

pBBR1 plasmid with constituitive lambda-CI (J23101-Bujard-Lambda-CI) and PirWT (PTet-B0033-PirWT) expression

Depositor

Thomas Gorochowski

Article

Hina-Nilesh Joshi et al ACS Synth Biol. 2026 Jan 13. doi: 10.102

Insert

LambdaCI-PirWT

Expression

Bacterial

Mutation

None

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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PlayBack-Ef1aKpn1

Plasmid

#203307

Purpose

For expression of eukaryotic genes of interest with Ef1a promoter with Kpn1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pOGG039

Plasmid

#113996

Purpose

SC module. Terminator for T7RNAP

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Terminator for T7RNAP

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pDD426

Plasmid

#202079

Purpose

Frame +1/-0 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame +1/-0 sgRNA

Use

CRISPR

Promoter

EF1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pND003

Plasmid

#200949

Purpose

Yeast surface display destination vector for Golden Gate cloning

Depositor

Timothy Whitehead

Article

Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

Type

Empty backbone

Use

Yeast surface display

Tags

AGA2, HA, and Myc

Expression

Yeast

Promoter

GAL1

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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