We narrowed to 10,665 results for: t7

1. modRNAc0-Cas9

   Plasmid

   #170180

   Purpose

   IVT template plasmid for making modified Cas9 RNA

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

   Insert

   Cas9
   

   Use

   Ivt template plasmid

   Promoter

   T7 promoter

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-Rsn2

   Plasmid

   #61686

   Purpose

   bacterial expression of ranaspumin 2 as a His-tagged fusion protein

   Depositor

   Brian Smith

   Article

   Mackenzie et al Biophys J. 2009 Jun 17;96(12):4984-92. d

   Insert

   Ranaspumin-2
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. His-DERA

   Plasmid

   #203225

   Purpose

   For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

   Depositor

   Vern Schramm

   Article

   Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

   Insert

   deoxyribose aldolase
   

   Tags

   N-terminal TEV cleavable 6xHis tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-DUET-1-mRhubarb720-HO

   Plasmid

   #141201

   Purpose

   Expresses mRhubarb720 fluorescent protein and heme oxygenase

   Depositor

   Elad Firnberg

   Article

   Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

   Inserts

   mRhubarb720
   heme oxygenase
   

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   R134H, L196Q, T202D, V203I, W309R, Q310A compared…

   Promoter

   T7

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRRL-VinculinCS

   Plasmid

   #200309

   Purpose

   Lentivirus vector expressing vinculin with FRET conformational sensor

   Depositor

   Brenton Hoffman

   Article

   Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

   Insert

   VinculinCS
   

   Use

   Lentiviral

   Promoter

   T7

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21a-trAtCCD8(pYL735)

   Plasmid

   #178286

   Purpose

   Expresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Insert

   trAtCCD8
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCellFree_G03 TCF12

   Plasmid

   #67113

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   TCF12 (TCF12 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. VV020: WT Arch-3 - eGFP in pET28b

   Plasmid

   #58488

   Purpose

   Expresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coli

   Depositor

   Adam Cohen

   Article

   Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

   Insert

   Archaerhodopsin-3
   

   Tags

   eGFP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. POLN_pDEST17

   Plasmid

   #64885

   Purpose

   bacterial expression of POLN for purification

   Depositor

   Richard Wood

   Article

   Takata et al Nucleic Acids Res. 2010 Jun;38(10):3233-

   Insert

   POLN (POLN Human)
   

   Tags

   Flag and His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDB007ns2a

    Plasmid

    #99214

    Purpose

    Accessory plasmid for PACE of PylRS variants; negative selection

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
    

    Mutation

    amber codons at positions 12 and 203 of T7 RNA po…

    Promoter

    Ppsp, PproK, and Ptet

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. OmpT

    Plasmid

    #213137

    Purpose

    OmpT_native membrane expression

    Depositor

    Sebastian Hiller

    Article

    Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

    Insert

    OmpT
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-Vinculin_TurboID_Venus

    Plasmid

    #200272

    Purpose

    Expressing vinculin with co-localization labeler TurboID and fluorescence reporter Venus

    Depositor

    Brenton Hoffman

    Article

    Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

    Insert

    Vinculin_TurboID_Venus
    

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMJ823

    Plasmid

    #39313

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9 (lin2744 Listeria innocua)
    

    Use

    CRISPR

    Tags

    MBP and thioredoxin-His6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3-Dronpa-Kras

    Plasmid

    #182329

    Purpose

    Plasma membrane-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    Dronpa-Kras
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21c-eIF4A

    Plasmid

    #37230

    Depositor

    Jon Lorsch

    Article

    Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

    Insert

    TIF1 (TIF1 Budding Yeast)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pMBP-LbCas12a D832A

    Plasmid

    #113517

    Purpose

    Expresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nuclease

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    LbCas12a
    

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    D832A

    Promoter

    T7

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. LacI INCENP

    Plasmid

    #45236

    Purpose

    Target INCENP to lac operator sequence

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    LacI, INCENP-Δcen, mCherry (lacI )
    

    Tags

    LacI (lac repressor protein) and mCherry

    Expression

    Mammalian

    Mutation

    CEN box deleted from INCENP (aa1-46)

    Promoter

    t7

    Available Since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lapronic/pRSETB

    Plasmid

    #140755

    Purpose

    FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

    Depositor

    Luis Felipe Barros

    Article

    Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

    Insert

    Lapronic (Lactate/PYRuvate Ratio Optical Nano-Indicator from CECs)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCoofy48

    Plasmid

    #55186

    Purpose

    Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-mScarlet-TEV-Strep-His

    Plasmid

    #210823

    Purpose

    Bacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent protein

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    mScarlet
    

    Tags

    TwinStrep, 10xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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