Skip to main content
Addgene
Showing: 481 - 500 of 501 results

  1. pDL026_HA-rb_IgG_LC_15F11

    Plasmid
    #193623
    Purpose
    HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.
    Depositor
    Jennifer DeLuca
    Article
    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:
    Insert
    Anti-HA_15F11 rabbit light chain
    Use
    Tags
    signal peptide
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193623-plasmid-map-sequence-id-380458

    Enlarge

  2. 4LNDDE488Q-pCDNA3.1(+)

    Plasmid
    #172228
    Purpose
    Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) in mammalian cells
    Depositor
    Joshua Rosenthal
    Article
    Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15
    Insert
    Lambda N peptide - human ADAR2 Deaminase Domain E488Q
    Use
    Tags
    6xHis and Flag
    Expression
    Mammalian
    Mutation
    changed the Glutamic acid 488 in the Deaminase Do…
    Promoter
    CMV
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    172228-plasmid-map-sequence-id-339581

    Enlarge

  8. pSP64-mHCN2

    Plasmid
    #53060
    Purpose
    Expresses alpha subunit of mouse HCN2 channel in Xenopus oocytes
    Depositor
    Michael Sanguinetti
    Article
    Chen et al J Biol Chem. 2000 Nov 17;275(46):36465-7
    Insert
    Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (Hcn2 Mouse)
    Use
    Xenopus oocyte expression
    Tags
    Expression
    Mutation
    E55G, R237H, and K283R polymorphisms compared to …
    Promoter
    SP6
    Available since
    Aug. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    53060-plasmid-map-sequence-id-82596

    Enlarge

  14. AAV-short_BrokenHeart (no BC)

    Plasmid
    #86950
    Purpose
    AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.
    Depositor
    Joseph Dougherty, Rob Mitra
    Article
    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    Brokenheart construct
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    86950-plasmid-map-sequence-id-172437

    Enlarge

  16. p Pcrg1: Khd4-Ada-Gfp (CbxR)

    Plasmid
    #203734
    Purpose
    Plasmid for ectopic integration and expression of Khd4-Ada-Gfp. The 4282 bp khd4-ORF is fused C-terminally to the 1200 bp adar catalytic domain (Ada) with three repeats of GGGGS linker in between, fol
    Depositor
    Michael Feldbrügge
    Article
    Sankaranarayanan et al Proc Natl Acad Sci U S A. 2023 Aug 22;12
    Insert
    Khd4-Ada-Gfp
    Use
    Tags
    Gfp
    Expression
    Bacterial
    Mutation
    Promoter
    Pcrg1
    Available since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    203734-plasmid-map-sequence-id-404932

    Enlarge

  17. EGFP-plk1-AKAP

    Plasmid
    #132967
    Purpose
    To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage
    Depositor
    Michael Yaffe
    Article
    Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.
    Insert
    polo-like kinase 1-AKAP (PLK1, AKAP9 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    C terminus of full-length Plk1 fused to the peric…
    Promoter
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    132967-plasmid-map-sequence-id-265937

    Enlarge

  18. NLS-4LNDDE488Q-pCDNA3.1(+)

    Plasmid
    #172314
    Purpose
    Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)
    Depositor
    Joshua Rosenthal
    Article
    Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:
    Insert
    Lambda N peptide - human ADAR2 Deaminase Domain E488Q
    Use
    Tags
    6xHis, Flag, and Nuclear Localization Signal of S…
    Expression
    Mammalian
    Mutation
    changed the Glutamic acid 488 in the Deaminase Do…
    Promoter
    CMV
    Available since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    172314-plasmid-map-sequence-id-339830

    Enlarge

  20. pcDNA3.1-PDGFRA-T674I-V5/HIS

    Plasmid
    #234756
    Purpose
    Expression of the T674I mutant variant of human PDGFRA, which is associated with Idiopathic Hypereosinophilic Syndrome, resistant to imatinib
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Insert
    PDGFRA-T674I receptor tyrosine kinase, full length (PDGFRA Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    T674I substitution
    Promoter
    CMV
    Available since
    April 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    234756-plasmid-map-sequence-id-468865

    Enlarge

Showing: 481 - 500 of 501 results