2,223 results

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pCS2_HA_dreAdar1

Plasmid

#250540

Purpose

In vitro transcription (SP6 promoter) or mammalian expression (CMV promoter) of the catalytic domain of zebrafish Adar1

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

HA_dreAdar1

Use

Synthetic Biology

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_cmlc2(myl7)_cmlc2-nmKate

Plasmid

#238374

Purpose

Drives expression of 3 different gRNAs targeting cmlc2 (myl7), and expression of nuclear mKate in cardiomyocytes.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting cmlc2

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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p5E itga11a-cFos

Plasmid

#240524

Purpose

5' Gateway Entry vector containing the itga11a-cFos promoter for fibroblast-specific expression in zebrafish

Depositor

Balázs Enyedi

Article

Fazekas et al J Cell Biol. 2026 Jan 5;225(1):e20250116

Inserts

integrin α11a - 3.2kb promoter (itga11a Zebrafish)
cFos - 216bp minimal promoter (Fos Mouse)

Use

Unspecified

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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UAS:syp-dL5-mNeonGreen

Plasmid

#241798

Purpose

Expresses synaptically localized dL5 protein fused to mNeonGreen, under control of UAS promoter

Depositor

Harold Burgess

Article

Sharma et al bioRxiv [Preprint]. 2025 Apr 5:2025.04.0

Inserts

sypb (sypb Zebrafish)
dL5
mNeonGreen (zebrafish codon optimized)

Use

Zebrafish transgenesis (tol1)

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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p160Tol2-her4.3:switchCas9

Plasmid

#227680

Purpose

Expression of cre inducible and her4.3 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs for SABER-seq in zebrafish

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

loxP-dsRed-loxP-Cas9-t2A-GFP
4xU6:sgRNA

Use

CRISPR

Promoter

U6 and her4.3

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR; cryaa:mCherry

Plasmid

#230043

Purpose

Partial ablation and subsequent lineage tracing of regenerating hepatocytes in zebrafish.

Depositor

Sumeet Pal Singh

Article

Eski et al Nat Commun. 2025 Jun 6;16(1):5260. doi:

Insert

lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR

Use

Cre/Lox; Zebrafish expression

Promoter

fabp10a

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2DY-EF

Plasmid

#221716

Purpose

Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2DY-EF (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

D651E and Y680F in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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hzHDAC10

Plasmid

#225542

Purpose

Expresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.

Depositor

David Christianson

Article

Herbst-Gervasoni et al ACS Chem Biol. 2020 Aug 21;15(8):2154-21

Insert

Histone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)

Tags

His6-MBP tag with cleavable TEV site

Expression

Bacterial

Mutation

Mutated A24E and D94A

Promoter

T7

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pXTm-zebrafish Tspan4a

Plasmid

#210327

Purpose

Expresses Tetraspanin-4a (TSPAN4a) as migrasome marker

Depositor

Li Yu

Article

Jiang et al Nat Cell Biol. 2019 Aug;21(8):966-977. d

Insert

Tetraspanin 4a (tspan4a Zebrafish)

Use

Fish expression

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAWO-SUMO-3C_14M_zTDP2

Plasmid

#200512

Purpose

Expression of zebrafish TDP2 recombinant protein

Depositor

Antony Oliver

Insert

TDP2 (tdp2b Zebrafish)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

L136N, A139S, C239T, K240R, K309Q, K314L, T315G, …

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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p5E_ngn1_LSE_C

Plasmid

#199395

Purpose

pDEL98; Gateway 5' Entry clone encoding select ngn1 upstream regulatory sequence

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

LSE_C

Use

Gateway Cloning

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2_NBT:Cas9green; erbb2_gRNA171

Plasmid

#199337

Purpose

pDEL134; transgenic construct to express cell-specific Cas9 in neurons; ubiquitous erbb2 sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

NBT
Cas9
erbb2 sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2_claudink:Cas9green; control_gRNA

Plasmid

#199336

Purpose

pDEL143; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous control sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

claudink
Cas9
control sgRNA

Use

Tol2 destination vector

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pCK051 p5E exorh:mCerulean

Plasmid

#195952

Purpose

Gateway p5E vector with reporter

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

exorh:mCerulean

Use

Other

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCK033 p5E desma MCS

Plasmid

#195948

Purpose

Gateway p5E vector

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

desmin a regulatory elements (desma Zebrafish)

Use

Other

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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p5E-pth2

Plasmid

#191428

Purpose

Tol2 Gateway 5' entry vector containing 2067 bp upstream of the CDS for zebrafish pth2 (ENSDARG00000022951), including the 5' UTR and first intron (GRCz11 chr 17:33493999-33496066)

Depositor

Timothy Erickson

Article

Venuto et al Biol Open. 2022 Sep 26. pii: 276788. doi

Insert

pth2 regulatory region (pth2 Zebrafish)

Use

Tol2 gateway 5' entry vector

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-olig1

Plasmid

#192744

Purpose

Gateway entry vector encoding zebrafish olig1

Depositor

Summer Thyme

Insert

olig1 (olig1 Zebrafish)

Use

Gateway Cloning

Mutation

F106L

Promoter

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf ctnna1L344P

Plasmid

#185407

Purpose

Zebrafish ctnna1 bearing a point mutation in the vinculin binding site. For in vitro transcription (SP6) or expression through CMV

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

ctnna1 (ctnna1 Zebrafish)

Use

In vitro synthesis of mrna

Expression

Mammalian

Mutation

changed Leucine 344 to Proline

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8-TwinStrep-3C-DrHDAC6 (40-831)

Plasmid

#180226

Purpose

Baculovirus transfer plasmid to express TwinStrep-3C-DrHDAC6 (40-831)

Depositor

Patrick Matthias

Article

Langousis et al Methods Mol Biol. 2023;2589:467-480. doi

Insert

Histone deacetylase 6 (hdac6 Zebrafish)

Tags

TwinStrep-3C

Expression

Insect

Promoter

polyhedrin

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-KalTA4-; HE1A:BFP -1

Plasmid

#180011

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; HE1A: BFP

Use

Synthetic Biology

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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