2,226 results

1. pCAG mEmerald-LAMP1

   Plasmid

   #168513

   Purpose

   Expression of rat LAMP1 fused with mEmerald to perform live tracking of lysosomes

   Depositor

   Franck Polleux

   Article

   Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGFP Cas

   Plasmid

   #50729

   Purpose

   mammalian expression of GFP-Cas. Note-This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

   Depositor

   Kenneth Yamada

   Insert

   Cas (Bcar1 Rat)
   

   Use

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. Kif5A-CIBN

   Plasmid

   #102251

   Purpose

   Expresses fusion of kinesin heavy chain 5A (1-572) and CIB1 (1-170)

   Depositor

   Bianxiao Cui

   Article

   Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

   Insert

   Kif5A-CIB1 (Kif5a, CIB1 Rat, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLVX-CMV-Galpha-i2 ONE-GO

   Plasmid

   #189736

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Inserts

   Galpha-i2 ONE-GO
   GNAI2 (Gnai2 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pLVX-hSyn-Galpha-i1 ONE-GO

   Plasmid

   #204333

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   hSyn Galpha-i1 ONE-GO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pGas-miRFP670nano

   Plasmid

   #127441

   Purpose

   G protein alpha-s internally tagged with miRFP670nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   G protein alpha-s-miRFP670nano
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. p159 pPr-luc

   Plasmid

   #8392

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

   Insert

   probasin promoter (Pbsn Rat)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 29, 2005

   Availability

   Academic Institutions and Nonprofits only

8. His-GFP-Rim2

   Plasmid

   #170869

   Purpose

   Mammalian expression of His-GFP-Rim2.

   Depositor

   Justin Taraska

   Article

   Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

   Insert

   Regulating synaptic membrane exocytosis protein 2 (Rim 2) (Rims2 Rat)
   

   Use

   Tags

   (His)6 and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-AAV-syn-jGCaMP7b-WPRE

   Plasmid

   #104489

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV Retrograde and AAV1

   Insert

   jGCaMP7b
   

   Use

   AAV

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. alpha1-EGFP

    Plasmid

    #118952

    Purpose

    GABAA receptor expression (alpha1 subunit). EGFP tag at the C-terminus.

    Depositor

    Angel de Blas

    Article

    Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.

    Insert

    GABRA1 (Gabra1 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBactin-PACT-mOrange2-hCM1

    Plasmid

    #196868

    Purpose

    Expression of Pericentrin-AKAP450 centrosomal targeting (PACT) domain fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). PACT targets CM1 to the centrosome

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    PACT-mOrange2-hCM1 (Akap9 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. C1-F-tractin-mCitrine

    Plasmid

    #155217

    Purpose

    Monitor global F-actin distribution

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine (Itpka Rat)
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt

    Plasmid

    #175180

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Has Service

    AAV1

    Insert

    pAAV hSyn FLEX iGluSnFR3 v857.PDGFR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin

    Available since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBa.Halo-myosin Va tail 1006-1828

    Plasmid

    #162715

    Purpose

    Mammalian expression of rat WT Myosin Va with deletion of the 1005 N-terminal residues

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    Myosin Va (Myo5a Rat)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    WT with deletion of the 1005 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. LZF97_hSyn-spine-jRGECO1a

    Plasmid

    #119198

    Purpose

    Targets jRGECO1a to spines in living neurons.

    Depositor

    Don Arnold

    Article

    Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

    Insert

    jRGECO1a-TPR3-Utrophin(1-78)-TPR3-Zfn(CCR5-L) (Utrn Rat, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human Synapsin 1

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOTTC763 - pX458 with rat Rosa26 gRNA A

    Plasmid

    #113161

    Purpose

    A plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFP

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat Rosa26
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLIC-myc-GBAi W211A

    Plasmid

    #171754

    Purpose

    For the mammalian expression of myc-GBAi W211A

    Depositor

    Mikel Garcia-Marcos

    Article

    Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

    Insert

    GBAi
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. p-AAV-sh[SNCA]

    Plasmid

    #75437

    Purpose

    knockdown alpha-synuclein in rat neurons

    Depositor

    Edward Burton

    Article

    Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

    Insert

    snca (Snca Rat)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-SYP1-miniSOG-T2A-mCherry

    Plasmid

    #50972

    Purpose

    AAV2 transfer vector containing the InSynC (SYP1-miniSOG-T2A-mCherry) construct

    Depositor

    Roger Tsien

    Article

    Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

    Insert

    Synaptophysin-miniSOG-T2A-mCherry (Syp Rat)
    

    Use

    AAV

    Tags

    T2A-mCherry and miniSOG

    Expression

    Mutation

    Promoter

    human synapsin promoter

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV.CAG.iGluSnFR3.v857.SGZ

    Plasmid

    #178334

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV CAG iGluSnFR3 v857.SGZ
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge