-
Plasmid#123734PurposeEncodes tamoxifen activated split-dCAS9 (N-Terminal) as a Type 3 part to be used in the MTK systemDepositorInsertspdCas9(1-204)-ERT2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK1_015
Plasmid#123678PurposeEncodes ConLS, chicken hypersensitivity site 4 insulator and locus of X-over P1 (loxP) as a Type 1 part to be used in the MTK systemDepositorInsertConnector LS-2xHS4-LoxP
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pT7-7 asyn WT
Plasmid#36046DepositorInsertalpha-synuclein (SNCA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
iCas
Plasmid#84232PurposeExpression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)DepositorInsertCas9
UseTags2A-OFP co-expression and ERT2-ERT2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYDS2.0_AT118-H
Plasmid#222846PurposeYeast-display plasmid encoding Nb. AT118-H, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)DepositorInsertAT118-H
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Ash2L full
Plasmid#15548DepositorInsertAsh2L (ASH2L Human)
UseTagsExpressionMammalianMutationR622H (Please see depositor comments below)PromoterAvailable sinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGW1-Myc-DJ1-WT
Plasmid#29347DepositorInsertDJ1 (PARK7 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
PJ-DEAD
Plasmid#38002DepositorUseTagsmRFPExpressionMammalianMutationAsp 1263 mutated to Ala and Cys 779 mutated to SerPromoterAvailable sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLEGFP-Y705F-STAT3
Plasmid#71445PurposeRetroviral expression of Y705F-STAT3. Please note that this plasmid contains Y705F-STAT3 tagged with FLAG, not GFP.DepositorInsertSTAT3 (STAT3 Human)
UseRetroviralTagsFLAGExpressionMutationY705FPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGPN-TMPRSS2
Plasmid#187484PurposeExpression vector - TMPRSS2DepositorInsertTMPRSS2 (TMPRSS2 Human)
UseLentiviralTagsExpressionMutationPromoterEFSAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-BTK-mCherry
Plasmid#50043PurposeExpression of BTK-mCherry in mammalian cellsDepositorInsertBTK (Btk Mouse)
UseRetroviralTagsmCherryExpressionMammalianMutationPromoterpCMVAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgAMPK alpha1 clone1
Plasmid#162119PurposeLentiviral sgRNA plasmid targeting human AMPK alpha1DepositorInsertsgAMPK alpha1 (PRKAA1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Luciferase-pcw107-V5
Plasmid#64649Purpose(control) when used to produce lentivirus, express physiological levels of insertDepositorInsertn/a
UseLentiviralTagsV5ExpressionMutationPromoterPGKAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
PJ-Sac
Plasmid#38000DepositorUseTagsmRFPExpressionMammalianMutationAsp 1263 mutated to AlaPromoterAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
PJ-INPP5E
Plasmid#38001DepositorUseTagsmRFPExpressionMammalianMutationCys 779 mutated to SerPromoterAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
IL2 pMalLP
Plasmid#41808DepositorInsertIL2 (IL2 Human)
UseTags3C protease site, 6x His, and Maltose Binding Pro…ExpressionBacterialMutationPromoterpTacAvailable sinceJan. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYDS2.0_AT118-L
Plasmid#222847PurposeYeast-display plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivityDepositorInsertAT118-L
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
p420-3
Plasmid#71257Purposeluciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoterDepositorInsert3 copies of the GM420 NFAT-AP-1 site (CSF2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterGM-CSF minimalAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
SynNotch TMD/Gal4-VP64 ICD (MTK 3b) (pAN1031)
Plasmid#194222PurposeMammalian Toolkit part 3b encoding SynNotch and GAL4-VP64 to be used in designing modular receptorsDepositorInsertSynNotch-GAL4 DBD-VP64 AD
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag hDPY30
Plasmid#15554DepositorInsertDPY30 (DPY30 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_LuxSit_i_mTagBFP2
Plasmid#194300PurposeMammalian expression of the de novo luciferase, LuxSit-iDepositorInsertLuxSit-i
UseTagsmTagBFP2ExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET26b_AT118-L-V5-His
Plasmid#222848PurposeE. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivityDepositorInsertAT118-L
UseTagsV5-HisExpressionBacterialMutationPromoterAvailable sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X1 Flag hDPY30
Plasmid#15556DepositorInsertDPY30 (DPY30 Human)
UseTagsFlag and GSTExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGPN-ACE2
Plasmid#187483PurposeExpression vector - ACE2DepositorInsertACE2 (ACE2 Human)
UseLentiviralTagsExpressionMutationPromoterEFSAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S129E
Plasmid#36050DepositorInsertalpha-synuclein S129E (SNCA Human)
UseTagsExpressionBacterialMutationS129EPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S129A
Plasmid#36048DepositorInsertalpha-synuclein S129A (SNCA Human)
UseTagsExpressionBacterialMutationS129APromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
N205 nLV EF-1a-ZFHD1-link-FKBP-HA <T2A> HP1aCS-Frbx2-V5-PGK-Blast
Plasmid#44017DepositorInsertsUseLentiviralTagsFrb, HA, Hu Frb x2(WT Frb+codon Wobble Frb), V5/e…ExpressionMutationN-term chromo domain removedPromoterEF-1aAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
Luciferase-pcw107
Plasmid#64648Purpose(control) when used to produce lentivirus, express physiological levels of insertDepositorInsertn/a
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -