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pAAV-syn-AICD-IRES-hrGFP

Plasmid

#107548

Purpose

high transduction efficiency AAV-mediated synapsin promoter-dependent expression of AICD (last 50 amino acids of APP) and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD (APP Human)

Use

AAV

Tags

Expression

Mutation

None

Promoter

human synapsin

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLgw ELK4-V5-EcoDam

Plasmid

#98607

Purpose

Mammalian DamID lentiviral vector for ELK4 with Dam-V5 using Gateway cloning

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ELK4 (Elk4 Mouse)

Use

Lentiviral; Damid

Tags

V5 and Dam (DNA adenine methyltransferase)

Expression

Mammalian

Mutation

Promoter

Heat Shock Minimal Promoter

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-AICD-NLS-IRES-hrGFP

Plasmid

#107543

Purpose

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Localisation Signal (NLS: CCAAAAAAGAAGAGAAAGGTA) at C-term end and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD-NLS (APP Human)

Use

AAV

Tags

Expression

Mutation

None

Promoter

CMV

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-AICD-NES-IRES-hrGFP

Plasmid

#107544

Purpose

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Export Signal (NES: CTCCCTCCACTAGAGCGACTAACCTTA) at C-term end and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD-NES (APP Human)

Use

AAV

Tags

Expression

Mutation

None

Promoter

CMV

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLgw EcoDam-V5-ELK4

Plasmid

#98606

Purpose

Mammalian DamID lentiviral vector for ELK4 with Dam-V5 using Gateway cloning

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ELK4 (Elk4 Mouse)

Use

Lentiviral; Damid

Tags

Dam (DNA adenine methyltransferase) and V5

Expression

Mammalian

Mutation

Promoter

Heat Shock Minimal Promoter

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRS_SN_400

Plasmid

#170472

Purpose

To amplify transcription template containing LacI operators

Depositor

Laura Finzi

Insert

T7A1, Os, O-, Lambda t1 in lacZ fragment

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7A1

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pYY_S2_400

Plasmid

#170471

Purpose

To amplify transcription template containing LacI operators

Depositor

Laura Finzi

Insert

T7A1, Os, O2, Lambda t1 in lacZ fragment

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7A1

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYY_N1_400

Plasmid

#170473

Purpose

To amplify transcription template containing LacI operators

Depositor

Laura Finzi

Insert

T7A1, O-, O1, Lambda t1 in lacZ fragment

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7A1

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS_2S_400

Plasmid

#170478

Purpose

To amplify transcription template containing LacI operators

Depositor

Laura Finzi

Insert

T7A1, O2, Os, Lambda t1 in lacZ fragment

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7A1

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS_2N_400

Plasmid

#170479

Purpose

To amplify transcription template containing LacI operators

Depositor

Laura Finzi

Insert

T7A1, O2, O-, Lambda t1 in lacZ fragment

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7A1

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-3e5

Plasmid

#106990

Purpose

T7 transcription template for 3e5

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

3e5

Use

Tags

Expression

Mutation

Promoter

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-5i3e5

Plasmid

#106995

Purpose

T7 transcription template for 5i3e5

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

5i3e5

Use

Tags

Expression

Mutation

Promoter

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-5iX

Plasmid

#106991

Purpose

T7 transcription template for 5iX

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

5iX

Use

Tags

Expression

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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T7-Xi3

Plasmid

#106992

Purpose

T7 transcription template for Xi3

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

Xi3

Use

Tags

Expression

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-3eX

Plasmid

#106993

Purpose

T7 transcription template for 3eX

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

3eX

Use

Tags

Expression

Mutation

Promoter

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-Xe5

Plasmid

#106994

Purpose

T7 transcription template for Xe5

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

Xe3

Use

Tags

Expression

Mutation

Promoter

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-5i3eX

Plasmid

#106996

Purpose

T7 transcription template for 5i3eX

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

5i3eX

Use

Tags

Expression

Mutation

Promoter

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-Xi3e5

Plasmid

#106997

Purpose

T7 transcription template for Xi3e5

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

Xi3e5

Use

Tags

Expression

Mutation

Promoter

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-Xi3eX

Plasmid

#106998

Purpose

T7 transcription template for Xi3eX

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

Xi3eX

Use

Tags

Expression

Mutation

Promoter

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG

Plasmid

#169416

Purpose

Used for guide cloning

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Type

Empty backbone

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge