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Showing: 481 - 500 of 3076 results

  7. pOGG013

    Plasmid
    #113987
    Purpose
    Vector construction module. Endlinker/terminator module to circularize plasmid following Part 3
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Endlinker/terminator Part 3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113987-plasmid-map-sequence-id-223414

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  8. pET-6xHis-GFP(+6)-L3

    Plasmid
    #205041
    Purpose
    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli
    Depositor
    Allie Obermeyer
    Article
    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021
    Insert
    GFP(+6)-L3
    Use
    Tags
    GRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGA
    Expression
    Bacterial
    Mutation
    E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    205041-plasmid-map-sequence-id-405654

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  9. pET-6xHis-GFP(+6)-H3

    Plasmid
    #205044
    Purpose
    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli
    Depositor
    Allie Obermeyer
    Article
    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021
    Insert
    GFP(+6)-H3
    Use
    Tags
    KRRRKKKRRRKKKRRRKK and MGHHHHHGGA
    Expression
    Bacterial
    Mutation
    E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    205044-plasmid-map-sequence-id-405657

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  10. pET-6xHis-GFP(0)-L3

    Plasmid
    #205035
    Purpose
    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli
    Depositor
    Allie Obermeyer
    Article
    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021
    Insert
    GFP(0)-L3
    Use
    Tags
    GRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGA
    Expression
    Bacterial
    Mutation
    E16R, I138R, D207R, N222K, L246R
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    205035-plasmid-map-sequence-id-405646

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  11. pET-6xHis-GFP(0)-H3

    Plasmid
    #205038
    Purpose
    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli
    Depositor
    Allie Obermeyer
    Article
    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021
    Insert
    GFP(0)-H3
    Use
    Tags
    KRRRKKKRRRKKKRRRKK and MGHHHHHGGA
    Expression
    Bacterial
    Mutation
    E16R, I138R, D207R, N222K, L246R
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    205038-plasmid-map-sequence-id-405651

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  13. ATX3-JD

    Plasmid
    #184247
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain fused with His-Tag (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    Ataxin-3 Josephin domain (ATXN3 Human)
    Use
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Premature stop codon V183X
    Promoter
    T7 promoter
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184247-plasmid-map-sequence-id-370313

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  14. TKIT NFL gRNA1 gRNA2

    Plasmid
    #182681
    Purpose
    Expression of spCas9, gRNA targeting intron 3 and gRNA targeting 3'UTR of the mouse Nefl gene. Can be used for the tagging of endogenous NFL via Targeted Knock-In with Two guides (TKIT) approach.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    two Nefl-targeting gRNAs, one targets intron 3 and the other 3'UTR of the Nefl gene (Nefl Mouse)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182681-plasmid-map-sequence-id-363020

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  16. CAG-GCaMP6f-3x-miR204-5p-3x-miR122-TS

    Plasmid
    #117384
    Purpose
    An AAV genome containing miRNA target sequences (TS) 204-5p and 122 to reduce expression of GCaMP6f in astrocytes and hepatocytes, respectively
    Depositor
    Viviana Gradinaru
    Article
    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:
    Insert
    GCaMP6f + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA ; 3 copies of miR122 : CAAACACCATTGTCACACTCCA
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    117384-plasmid-map-sequence-id-225349

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  17. ATX3-D1

    Plasmid
    #184246
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain and UIM1 fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    Ataxin-3 Josephin domain and UIM1 (ATXN3 Human)
    Use
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Premature stop codon N263X
    Promoter
    T7 promoter
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184246-plasmid-map-sequence-id-370314

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  18. pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA

    Plasmid
    #105000
    Purpose
    Expresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
    Depositor
    Jeffrey Chao
    Article
    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.
    Insert
    Renilla luciferase
    Use
    Tags
    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TetCMV
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105000-plasmid-map-sequence-id-203163

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Showing: 481 - 500 of 3076 results