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Fus-BcLOV-mCh

Plasmid

#208277

Purpose

Expresses Fus-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Publication

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Fus-BcLOV-mCh

Expression

Mammalian

Promoter

pCMV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC-SmBiT

Plasmid

#207632

Purpose

A plasmid encoding photocaged SpyCatcher (pSC) fused to SmBiT

Depositor

Cole DeForest

Publication

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

photocaged SpyCatcher-SmBiT

Tags

6xHis

Expression

Bacterial

Mutation

Amber stop codon at SC's critical lysine

Promoter

T7

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpH-HsNot9_S

Plasmid

#147494

Purpose

Bacterial Expression of HsNot9

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot9 (CNOT9 Human)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHRi_GPa3b17 TCRα

Plasmid

#187601

Purpose

Lentiviral expression of hTCR alpha (GPa3b17) for low expression

Depositor

Simon Davis

Publication

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

GPa3b17 hTCR alpha (TRA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mHSP

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pMG343

Plasmid

#176183

Purpose

preassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 marker

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

TPK2+ccdB dropout cassette

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pETDuet-1 TIM8,13

Plasmid

#170282

Purpose

Expresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

Depositor

Paul Schanda

Publication

Sucec et al Sci Adv. 2020 Dec 18;6(51). pii: 6/51/ea

Insert

yeast Tim8 and Tim13 (TIM8 Budding Yeast)

Tags

TEV-cleavable N-terminal His tag on Tim13 (on bac…

Expression

Bacterial

Available since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pF(UG) CamKII sf iGluSnFR

Plasmid

#158769

Purpose

iGluSnFR glutamate sensor containing a Golgi export sequence and and an ER exit motif for improved trafficking to the plasma membrane.

Depositor

Edwin Chapman

Publication

Vevea et al Elife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

iGluSnFR

Use

Lentiviral

Mutation

Additional ER and Golgi export sequences

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUPD2 AlcR (GB1550)

Plasmid

#160585

Purpose

Ethanol dependent transcrition factor

Depositor

Diego Orzaez

Insert

AlcR

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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AlphaD

Plasmid

#124526

Purpose

pColE1_AlphaD

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial, Insect, and Mamm…

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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T/brachyury(2A-Dre)

Plasmid

#140761

Purpose

Targeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.

Depositor

Hideyuki Okano

Publication

Serizawa et al Development. 2019 Nov 4;146(21). pii: de

Insert

Dre

Use

Mouse Targeting

Promoter

Endogenous T/brachyury

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pFL His-SDS22

Plasmid

#113512

Purpose

Expression of His-SDS22 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Publication

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

SDS22 (PPP1R7 Human)

Tags

6*His

Expression

Insect

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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KG#59

Plasmid

#110929

Purpose

Expresses proteins pan-neuronally in C. elegans (entire nervous system)

Depositor

Kenneth Miller

Publication

Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

Inserts

rab-3 promoter
unc-54 3' control region

Expression

Bacterial

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac204-T|C-iGFP-PH(OSH1)

Plasmid

#105280

Purpose

Overexpresses iGFP-PH(Osh1) under the TPI1 promoter. Integrating at TRP1.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

PH domain of Osh1 (SWH1 Budding Yeast)

Tags

iGFP

Expression

Yeast

Promoter

TPI1

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pWT055h

Plasmid

#96867

Purpose

sgRNA-HEK4

Depositor

David Liu

Publication

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA-HEK4

Expression

Mammalian

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19-P70a-Top-T~Bottom-T

Plasmid

#87315

Purpose

Control plasmid. Encodes the Split-Broccoli system (Top + Bottom) with only "Top" under the control of E. coli sigma 70 promoter.

Depositor

Donald Burke

Publication

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system (promoter control)

Use

Synthetic Biology

Expression

Bacterial

Promoter

P70a

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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SLC2A3

Plasmid

#74649

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

SLC2A3 (SLC2A3 Human)

Use

Baculovirus

Tags

TEV-His10-Flag

Promoter

PH

Available since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
TOPO pGK

Plasmid

#68452

Purpose

TOPO construct expressing pGK for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Phosphoglycerate Kinase 1 Promoter (PGK1 Human)

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Snail1-3'UTR

Plasmid

#61781

Purpose

Luciferase reporter containing the 3'UTR of mouse Snail1

Depositor

Judy Lieberman

Publication

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Snail1 3'UTR

Use

Luciferase

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
LI B-C T-Saphire

Plasmid

#54424

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

T-saphhire + 16 aa GS linker

Use

Cloning vector

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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LI B-C mOrange

Plasmid

#54427

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mOrange + 16 aa GS linker

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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