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pDGE463

Plasmid

#153234

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE651

Plasmid

#153229

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRC1765

Plasmid

#141346

Purpose

Mini plasmid containing minimal sequences for amplification in bacteria and two BbvCI nicking sites for introduction of modified DNA through annealing and ligation (e.g. mismatch, DNA lesions, flap).

Depositor

Rafael Fernandez-Leiro

Article

Fernandez-Leiro et al Nat Struct Mol Biol. 2021 Apr;28(4):373-

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-14-Mfn1-T562A

Plasmid

#191976

Purpose

To produce mutants mimicking dephosphorylated Mfn1

Depositor

Luca Scorrano

Article

Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

Insert

Mfn1 T562A (Mfn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

T562A

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-14-Mfn1-T562D

Plasmid

#191977

Purpose

To produce mutants mimicking phosphorylated Mfn1

Depositor

Luca Scorrano

Article

Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

Insert

Mfn1 T562D (Mfn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

T562D

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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∆C' terminal-DPYSL2-pLJC2

Plasmid

#189867

Purpose

Expresses human DPYSL2 (CRMP2) lacking the C' terminus (491-572 aa), in mammalian cells

Depositor

Yoav Shaul

Article

Abu Rmaileh et al J Cell Biol. 2022 Jul 4;221(7). pii: 213

Insert

ΔC-DPYSL2

Use

Lentiviral

Tags

3X FLAG

Expression

Mammalian

Mutation

deleted the C'terminus of DPYSL2 (491-572 aa)

Promoter

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pDGE652

Plasmid

#153230

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Hiv7CMV-Cremyc-2A-GFP

Plasmid

#117148

Purpose

Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.

Depositor

Qiang Lu

Article

Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:

Insert

Cremyc-2A-GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDGE671

Plasmid

#153237

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Str-KDEL_ManII-SBP-tagBFP

Plasmid

#65254

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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pLY239

Plasmid

#184152

Purpose

Reprogrammed short tracrRNA generator for hijacking RFP mRNA as CRISPR RNA (target site 3)

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-tracrRNA-R3

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLY273

Plasmid

#184155

Purpose

Reprogrammed short tracrRNA for hijacking mRNA of zraP

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-tracrRNA-zraP-22

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCheck2 Dest (R1-R2) #BGV129

Plasmid

#48955

Purpose

Destination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors.

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Luciferase; Gateway cloning vector

Tags

Expression

Mutation

Promoter

SV40

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-TTF-1-AD1-deletion

Plasmid

#75265

Purpose

Expresses a deletion mutant of human TTF-1 lacking the activation domain 1

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

TTF-1 (NKX2-1 Human)

Use

Tags

none

Expression

Mammalian

Mutation

AD1 (activation domain 1) deleted

Promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-TTF-1-AD2-deletion

Plasmid

#75266

Purpose

Expresses a deletion mutant of human TTF-1 lacking the activation domain 2

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

TTF-1 (NKX2-1 Human)

Use

Tags

none

Expression

Mammalian

Mutation

AD2 (activation domain 2) deleted

Promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pGFP-Ub-VPS13D_dC

Plasmid

#176466

Purpose

express VPS13D lacking C-terminal 407 aa in mammalian cells

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Use

Tags

GFP-Ub

Expression

Mammalian

Mutation

C-terminal 407 aa is deleted.

Promoter

CMV

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 CSNK2A1

Plasmid

#67085

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CSNK2A1 (CSNK2A1 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pDGE673

Plasmid

#153239

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
A102 ΔAmpH Δ2x+ve

Plasmid

#129484

Purpose

Expression of Glo3 ΔAmpH lacking the COPI-binding motif in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pUC_delta ald1

Plasmid

#126708

Purpose

vector for knocking out ald1 in Komagataella phaffii

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

HIS4

Use

Tags

Expression

Yeast

Mutation

Promoter

His

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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