We narrowed to 11,977 results for: pcDNA

1. pcDNA3-PKCsub(T/A)-YPet-NES

   Plasmid

   #138208

   Purpose

   Encodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   PKCsub(T/A)-YPet-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available since

   Sept. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-mouse cdk6 R31C

   Plasmid

   #75171

   Purpose

   Mammalian expression of mouse cdk6 with R31C

   Depositor

   Martine Roussel

   Insert

   cyclin dependent kinase 6 (Cdk6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R31C

   Promoter

   CMV

   Available since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3-HA-RIPK3 2KR(89,501)

   Plasmid

   #78812

   Purpose

   To overexpress RIPK3 2KR(89,501) in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK3 (RIPK3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K89R, K501R

   Promoter

   CMV

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-hRIPK1-Flag-K599R

   Plasmid

   #112490

   Purpose

   Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

   Depositor

   Junying Yuan

   Insert

   RIPK1 (RIPK1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K599R

   Promoter

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3 humanN-Flag SadBS

   Plasmid

   #66881

   Purpose

   Mammalian expression vector for expressing N-terminally Flag-tagged short isoform of SadB

   Depositor

   Maria Alvarado-Kristensson

   Article

   Eklund et al J Biol Chem. 2014 Aug 1;289(31):21360-73

   Insert

   SadB (BRSK1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3 PARL-FLAG-CT H335G

   Plasmid

   #13749

   Depositor

   Luca Pellegrini

   Article

   Cipolat et al Cell. 2006 Jul 14. 126(1):163-75.

   Insert

   Presenilin-associated rhomboid-like (PARL, PARL Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed catalytic His 335 to Gly

   Promoter

   Available since

   Feb. 1, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA5/FRT-Flag-EloB

   Plasmid

   #31983

   Purpose

   Mammalian expression of Flag-tagged Elongin B

   Depositor

   Joan Conaway

   Article

   Mahrour et al J Biol Chem. 2008 Mar 21;283(12):8005-13

   Insert

   Elongin B (Tceb2 Rat)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2013

   Availability

   Academic Institutions and Nonprofits only

8. 835 pcDNA3 HA PTEN G129R

   Plasmid

   #10929

   Depositor

   William Sellers

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   G129R

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1 2xFLAG-2xSTREP EMI1

   Plasmid

   #158947

   Purpose

   Express human EMI1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

   Insert

   EMI1 (FBXO5 Human)
   

   Use

   Tags

   2X FLAG 2X STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-HA-REDD1 S103L (#624)

    Plasmid

    #99942

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S103L

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA-FRT-FAK-kinase10

    Plasmid

    #59123

    Purpose

    FAK fret control sensor without FERM domain with 10 nm linker

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-AMPKsub-YPet-Kras

    Plasmid

    #84638

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub-YPet-Kras
    

    Use

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNAM-human eIF2C1-WT-Myc

    Plasmid

    #50360

    Purpose

    Expression of human eIF2C1 in mamalian cells

    Depositor

    Kumiko Ui-Tei

    Article

    Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

    Insert

    eIF2C1 (AGO1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA4/TO-mu24-ORF24-2xStrep

    Plasmid

    #138453

    Purpose

    Expresses mu24-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    mu24-ORF24 (ORF24 MHV68/KSHV)
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    mu24 amino acids 1-191 fused to ORF24 amino acids…

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-h-mmPtch1-B-NNQ-HA-GFP

    Plasmid

    #121164

    Purpose

    Expresses mmPtch1-B NNQ variant

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    Ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291, N…

    Promoter

    CMV

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-h-mmPtch1-B-I766FV111FL114F-HA-GFP

    Plasmid

    #120909

    Purpose

    Expresses Ptch1-B (I766FV111FL114F)

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    Ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291

    Promoter

    CMV

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO HIS DNAJB2b

    Plasmid

    #19551

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB2b
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-Dest53-SHP-1 S591D

    Plasmid

    #21803

    Depositor

    Stephen Shaw

    Article

    Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

    Insert

    SHP-1 (PTPN6 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    changed Serine 591 to Aspartic acid

    Promoter

    Available since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-FLAG-Fos Y345A

    Plasmid

    #8970

    Depositor

    John Blenis

    Article

    Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

    Insert

    c-Fos (Fos Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y345A

    Promoter

    Available since

    Sept. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-FLAG-RIPK3 4KR

    Plasmid

    #78821

    Purpose

    To overexpress RIPK3 4KR in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K55R, K89R, K363R, K501R

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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