Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 7,724 results for: Pol

Clear keywords
Showing: 5001 - 5020 of 7724 results

  1. RNF168-Halo HRD

    Plasmid
    #207080
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.
    Depositor
    Jens Schmidt
    Publication
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
    Expression
    Mammalian
    Promoter
    None
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207080-plasmid-map-sequence-id-432323

    Enlarge

  2. Lyn11-BirA*-HA

    Plasmid
    #214474
    Purpose
    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with an N-terminal Lyn11 plasma membrane localization sequence and C-terminal HA tag.
    Depositor
    Natalie Ahn
    Publication
    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.
    Insert
    BirA(R118G)
    Use
    Gateway Cloning and Lentiviral
    Tags
    HA tag and Lyn11 (Plasma Membrane Localization Se…
    Expression
    Mammalian
    Mutation
    R118G
    Promoter
    EF-1a
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214474-plasmid-map-sequence-id-425600

    Enlarge

  3. pFBD-BirA_CDC20:161-P477

    Plasmid
    #210888
    Purpose
    Insect expression of human CDC20 fragment S161 to P477. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Publication
    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.
    Insert
    CDC20:S161-P477 (CDC20 Human)
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210888-plasmid-map-sequence-id-424502

    Enlarge

  7. pPTG-GG-sg2+sg3

    Plasmid
    #127199
    Purpose
    Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter
    Depositor
    Jordan Green
    Publication
    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tRNA-gRNA
    Use
    Empty sgrna plasmid
    Expression
    Mammalian
    Promoter
    U6
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    127199-plasmid-map-sequence-id-248025

    Enlarge

  9. pMpGWB419

    Plasmid
    #68684
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Publication
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Promoter
    Cauliflower mosaic virus 35S promoter
    Available since
    July 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    68684-plasmid-map-sequence-id-121817

    Enlarge

  13. pSN-A112C-CP8B1

    Plasmid
    #191240
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Publication
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background) (CYP8B1 Staphylococcus aureus, Human)
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191240-plasmid-map-sequence-id-380472

    Enlarge

  17. ATM-Halo HRD

    Plasmid
    #207082
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous ATM locus.
    Depositor
    Jens Schmidt
    Publication
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human ATM locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    None
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207082-plasmid-map-sequence-id-430992

    Enlarge

  19. pCMV-SEMPER_GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid
    #221076
    Purpose
    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication
    Depositor
    Mikhail Shapiro
    Publication
    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    221076-plasmid-map-sequence-id-438459

    Enlarge

  20. pCMV-SEMPER_CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid
    #221077
    Purpose
    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication
    Depositor
    Mikhail Shapiro
    Publication
    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    221077-plasmid-map-sequence-id-438460

    Enlarge

Showing: 5001 - 5020 of 7724 results