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We narrowed to 6,293 results for: cat.2

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Showing: 5001 - 5020 of 6293 results

  2. pJC8

    Plasmid
    #170757
    Purpose
    Co-express in mammalian cells two target proteins C-terminally fused to V5 and FLAG tags respectively, using an internal ribosome entry site and two multi-cloning sites.
    Depositor
    Roberto Dominguez
    Article
    Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi
    Type
    Empty backbone
    Tags
    site 1- V5 tag and site 2- FLAG tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    170757-plasmid-map-sequence-id-334720

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  3. pMito-MtrxRFP2

    Plasmid
    #172324
    Purpose
    fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)
    Depositor
    Huiwang Ai
    Article
    Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.
    Insert
    mitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
    Tags
    His-tag and Mitochondria localization sequence
    Expression
    Mammalian
    Promoter
    CMV, SP6
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172324-plasmid-map-sequence-id-431104

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  5. Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pcDNA3

    Plasmid
    #206121
    Purpose
    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domains I, II, III and IV to to abolish divalent cation binding
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1a (Cacna1a Rat)
    Expression
    Mammalian
    Mutation
    E320A, E670A, E1411A, E1707A
    Promoter
    CMV
    Available Since
    Oct. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    206121-plasmid-map-sequence-id-410287

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  6. pB-T-PAF-VLDLR_ecto_short isoform

    Plasmid
    #215395
    Purpose
    Expression of the ectodomain(1-797) of the short isoform of the Very Low-Density Lipoprotein Receptor (VLDLR, UniProtKB; P98155-2) fused to a C-tag (EPEA) in th C- terminal for purification.
    Depositor
    Franz-Ulrich Hartl
    Insert
    VLDLR (VLDLR Human)
    Tags
    C-tag (EPEA)
    Expression
    Mammalian
    Mutation
    p.Ala798_Ala873del
    Promoter
    tetO
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215395-plasmid-map-sequence-id-431788

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  11. pUDP044

    Plasmid
    #101168
    Purpose
    pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Insert
    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    ScTDH3
    Available Since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101168-plasmid-map-sequence-id-199309

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  13. pTO_CHMP4B_LAP

    Plasmid
    #101850
    Purpose
    Tet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    Charged Multivesicular Body Protein 4B (CHMP4B Human)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    101850-plasmid-map-sequence-id-200248

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  16. pcDNA3-Flag-Tet2 CD Mut

    Plasmid
    #72220
    Purpose
    Express flag-tagged mouse Tet2 catalytic domain - catalytic dead mutant.
    Depositor
    Yi Zhang
    Article
    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do
    Insert
    Ten-Eleven Translocation 2 (Tet2 Mouse)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    The plasmid expresses TET2 catalytic domain (cata…
    Promoter
    CMV
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only

  17. p11_AaC60αβ/C20

    Plasmid
    #229506
    Purpose
    Expresses Anthoceros agrestis Chaperonin 60α, Chaperonin 60β, Chaperonin 20
    Depositor
    Laura Gunn
    Article
    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.
    Inserts
    Chaperonin 60α
    Chaperonin 60β
    Chaperonin 20
    Expression
    Bacterial
    Mutation
    N terminus chloroplast transit peptide truncation
    Promoter
    T7
    Available Since
    June 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229506-plasmid-map-sequence-id-456756

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  20. pSLiP-G1G2

    Plasmid
    #188965
    Purpose
    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown
    Depositor
    Seung-Goo Lee
    Article
    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131
    Inserts
    dCas9
    sgRNA 1: atcggtcgcattgttttccactagg, sgRNA 2: gttagacgctgattacatggactagg
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PrhaBAD
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    188965-plasmid-map-sequence-id-374348

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Showing: 5001 - 5020 of 6293 results