We narrowed to 24,502 results for: crispr
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Plasmid#204289PurposeFor marking CRISPR HDR integrant with EGFP expressed in flight muscles.DepositorInsertMHC-EGFP+H3:H26
UseCRISPRPromoterMHCAvailable SinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
hgRNA-A21_pLKO-Hyg
Plasmid#100559PurposehgRNA-A21 in a lentiviral backboneDepositorInserthgRNA-A21
UseCRISPR, Lentiviral, and Synthetic BiologyExpressionMammalianPromoterU6Available SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
ATM gRNA (BRDN0001146099)
Plasmid#77530Purpose3rd generation lentiviral gRNA plasmid targeting human ATMDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ATM gRNA (BRDN0001149033)
Plasmid#77531Purpose3rd generation lentiviral gRNA plasmid targeting human ATMDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
AKT1 gRNA (BRDN0001146935)
Plasmid#75500Purpose3rd generation lentiviral gRNA plasmid targeting human AKT1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-JMJD2A-Hygro
Plasmid#104410Purposetransient expression of dCas9-JMJD2A fusion proteinDepositorAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti-UCOE-dCas9-BFP-Zim3-KRAB
Plasmid#196716PurposeCRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3DepositorInsertdCas9-TagBFP-KRAB(ZIM3)
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSMutationD10A, N863APromoterEF1aAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262B-ABE8e
Plasmid#161524PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editingDepositorInsertTadA8e-zSpRYCas9(D10A)
UseCRISPR; Gateway compatible tada8e-zsprycas9(d10a)…ExpressionPlantAvailable SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18
Plasmid#172318PurposeCRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSP64T-hLbCpf1
Plasmid#107187Purposeplasmid to carry out IVT of human codon optimized LbCpf1 (based on Addgene 69988)DepositorInserthLbCpf1
UseCRISPRTagsNLS-3xHAPromotersp6Available SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-Cas12a-recX
Plasmid#158714PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses FnCpf1, NHEJ machinery of M. marinum and recXDepositorInsertrecX
UseCRISPRExpressionBacterialPromoterPmycAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-Cas12a
Plasmid#158713PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis, expresses FnCpf1, NHEJ machinery of M. marinumDepositorInsertthe MmNHEJ machinery (MMAR_4573, MMAR_4574, and MMAR_4575)
UseCRISPRExpressionBacterialAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
p(U6a-BsaI-gRNA)
Plasmid#65955PurposeTribolium U6a promoter with BsaI cloning site to insert guide RNA sequenceDepositorTypeEmpty backboneExpressionInsectPromoterU6Available SinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c-ddcpf1-rfp
Plasmid#153038PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.DepositorInsertAscpf1
ExpressionBacterialMutationE993AAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)
Plasmid#115487PurposeCRISPR Cas9 SunTag system to target NtDRMcd to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pV1393
Plasmid#111430PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
XLone-PE2
Plasmid#136463PurposeExpresses prime editing 2 (PE2) enzyme upon doxycycline treatmentDepositorInsertPE2
ExpressionMammalianPromoterTRE3GSAvailable SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-mTagBFP2-Blasticidin
Plasmid#167207PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with BlasticidinDepositorTypeEmpty backboneUseCRISPRTagsmTagBFP2ExpressionMammalianAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sa-dCas9-NLS-3xFLAG/pcDNA3.1
Plasmid#98041PurposeExpresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interestDepositorInsertSa-dCas9-NLS-3xFLAG
UseCRISPRTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMammalianMutationD10A + H557APromoterCMVAvailable SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)sgRNA_CAG-Cas9-venus-bpA
Plasmid#86986PurposeFor cloning and expression of sgRNA together with expression of a Cas9-Venus fusion proteinDepositorInsertCas9-venus
TagsCas9-Venus fusion proteinExpressionMammalianPromoterCAGAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only