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We narrowed to 25,138 results for: crispr

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Showing: 5001 - 5020 of 25138 results

  1. mAC-POLR2A donor (Hygro)

    Plasmid
    #124496
    Purpose
    A donor plasmid for tagging the N-terminus of human POLR2A with mAID-mClover
    Depositor
    Masato Kanemaki
    Publication
    Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    POLR2A homology arms and Hygro-P2A-mAID-mClover (POLR2A Human)
    Use
    CRISPR
    Tags
    mAID-mClover
    Available since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124496-plasmid-map-sequence-id-243723

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  4. pJM149

    Plasmid
    #134168
    Purpose
    Retroviral plasmid expressing GFP - Nde1
    Depositor
    Iain Cheeseman
    Publication
    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2
    Insert
    Nde1_SSSC E118A R129A E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    SSSC E118A R129A, E. coli codon optimized, CRISPR…
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    134168-plasmid-map-sequence-id-271754

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  5. LentiGuide-Puro-P2A-EGFP_mRFPstuf

    Plasmid
    #137730
    Purpose
    Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA
    Depositor
    Fredrik Wermeling
    Publication
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, Mouse Targeting, and Syntheti…
    Expression
    Bacterial and Mammalian
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    137730-plasmid-map-sequence-id-310820

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  10. pJR101

    Plasmid
    #187241
    Purpose
    Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Publication
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
    Use
    Lentiviral
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    187241-plasmid-map-sequence-id-374769

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  14. wtBxb1

    Plasmid
    #222337
    Purpose
    Plasmid for expressing wild type Bxb1 in mammalian cells
    Depositor
    David Liu
    Publication
    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon-optimized wild type Bxb1
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    222337-plasmid-map-sequence-id-437334

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  17. pJR100

    Plasmid
    #187240
    Purpose
    Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-BFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Publication
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
    Use
    Lentiviral
    Available since
    Oct. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    187240-plasmid-map-sequence-id-374768

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  20. Fragment1 with TLS2

    Plasmid
    #196983
    Purpose
    Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.
    Depositor
    Friedrich Kragler
    Publication
    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Intermediate fragment for assembly of gRNA-TLS2 construct
    Use
    Gateway Cloning
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196983-plasmid-map-sequence-id-390457

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Showing: 5001 - 5020 of 25138 results