We narrowed to 8,169 results for: gnal

1. DN1-Gli3 in AINGpCAGGS

   Plasmid

   #121970

   Purpose

   medium Gli3 transcriptional activator

   Depositor

   James Briscoe

   Article

   Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

   Insert

   DN2Gli3 (GLI3 Human)
   

   Use

   Chick electroporation

   Tags

   5xmyc

   Expression

   Mammalian

   Mutation

   N terminus deletion (nt1-864)

   Promoter

   Available since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPGK-T7/2-CD44s (-cyt)

   Plasmid

   #137815

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44s (-cyt) (CD44 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   without cytoplasmic region

   Promoter

   PGK

   Available since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. KCNE2_pcDNA6.2/EmGFP-Bsd

   Plasmid

   #176980

   Purpose

   Mammalian expression vector encoding KCNE2 and EmGFP-Bsd

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   KCNE2 (KCNE2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPGK-T7/2-CD44v6-10 (-cyt)

   Plasmid

   #137821

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v6-10 (-cyt) (CD44 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   without cytoplasmic region

   Promoter

   PGK

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCR3-FLAG-Gephyrin GC

   Plasmid

   #68818

   Purpose

   Flag tagged mammalian expression of GC domain

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   NIL

   Promoter

   CMV

   Available since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-RIαΔ(D/D+linker)-EGFP

   Plasmid

   #181843

   Purpose

   GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   RIα-(D/D+linker)EGFP (PRKAR1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of residues 12-112 in PKA RIα.

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag Depdc5 pLJM1

   Plasmid

   #46336

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

   Insert

   Depdc5 (DEPDC5 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Differs from Depdc5 isoform 1 by 10 amino acids …

   Promoter

   Available since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231G

   Plasmid

   #113953

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Use

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   delete N-terminal 21 residues, I132S, S212N, and …

   Promoter

   cmv

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-CaMKIIa-SpyTag-C1C2-mCherry

   Plasmid

   #69833

   Purpose

   Lentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing SpyTag-C1C2-mCherry fusion. SpyTag can be used to monitor membrane localization of the C1C2 opsin.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

   Insert

   SpyTag-C1C2
   

   Use

   Lentiviral

   Tags

   Trafficking signal and mCherry

   Expression

   Mammalian

   Mutation

   Inserted SpyTag after the signal peptide of C1C2.

   Promoter

   CamKIIa

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLJM60-FLAG-SLC38A9.1 I68A

    Plasmid

    #71864

    Purpose

    lentiviral stable expression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    SLC38A9.1 (SLC38A9 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized, Isoleucine 68 mutated to Alanine

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pMG058 MBP-GS 634-666-His

    Plasmid

    #154077

    Purpose

    Expresses MBP-GS 634-666-His (human 33aa Glycogen Synthase peptide as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    GYS1 (GYS1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGS-CD64-2

    Plasmid

    #109191

    Purpose

    Encodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    Full-length engineered CD64, derived from human CD64
    

    Use

    Tags

    Twin-Strep

    Expression

    Insect

    Mutation

    engineered for efficient expression, see publicat…

    Promoter

    Polyhedrin

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5 Flag Grb10 5A short

    Plasmid

    #37484

    Depositor

    David Sabatini

    Article

    Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

    Insert

    Grb10 5A (GRB10 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    isoform C (missing N-term 58aa), 536aa, S150A T15…

    Promoter

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1_mGFP-FGFR3-G380R

    Plasmid

    #191755

    Purpose

    Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-G380R mutation (associated with ACH; target mutation: c.1138G>A; silent mutation added on purpose: c.1131C>T)

    Depositor

    Irene Tiemann-Boege

    Article

    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

    Insert

    mGFP-FGFR3IIIc
    

    Use

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    c.1138G>A (G380R) + c.1131C>T (silent mutat…

    Promoter

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNL-CEF-IgM

    Plasmid

    #187013

    Purpose

    Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgM

    Depositor

    Hermann Eibel

    Article

    Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

    Insert

    Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgM
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28-p53 (1-90) (72P)

    Plasmid

    #62080

    Purpose

    Expression of human p53 (1-90) (72P) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD/PP residues 1-90; 72P

    Promoter

    T7

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA

    Plasmid

    #51255

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248 mutated to Ala

    Promoter

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA6-N-3XFLAG-Fzd6

    Plasmid

    #123588

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    frizzled class receptor 6 (Fzd6 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6

    Plasmid

    #192788

    Purpose

    aGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    antiGFP nanobody enhancer
    

    Use

    Tags

    H6 and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5 Flag Grb10 9A long

    Plasmid

    #37485

    Depositor

    David Sabatini

    Article

    Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

    Insert

    Grb10 9A (GRB10 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    isoform A, 594aa, S104A S150A T155A S158A S426A S…

    Promoter

    Available since

    Aug. 30, 2012

    Availability

    Academic Institutions and Nonprofits only