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pML104-HIS3-2

Plasmid

#232881

Purpose

Plasmid expressing Cas9 and gRNA CCAAGTTCGACAACTGCGTA which targets the HIS3 gene.

Depositor

Jolanda Van Leeuwen

Publication

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

HIS3 gRNA (HIS3 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-ADE13

Plasmid

#232887

Purpose

Plasmid expressing Cas9 and gRNA GTAGCAGCAAAAGAAGACAA which targets the ADE13 gene.

Depositor

Jolanda Van Leeuwen

Publication

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

ADE13 gRNA (ADE13 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-LAS17

Plasmid

#232892

Purpose

Plasmid expressing Cas9 and gRNA AATACCCTGAATTCTGCCGG which targets the LAS17 gene.

Depositor

Jolanda Van Leeuwen

Publication

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

LAS17 gRNA (LAS17 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML104-LEU2-2

Plasmid

#232883

Purpose

Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene.

Depositor

Jolanda Van Leeuwen

Publication

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

LEU2 gRNA (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-INO80

Plasmid

#232890

Purpose

Plasmid expressing Cas9 and gRNA GGAAGTAGAATCATTCGTAG which targets the INO80 gene.

Depositor

Jolanda Van Leeuwen

Publication

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

INO80 gRNA (INO80 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pL0_EF-Fld

Plasmid

#202181

Purpose

Level 0 plasmid containing a terminator sequence from gene 23658 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Fld terminator

Use

Synthetic Biology

Mutation

None

Available since

Feb. 2, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEG302-LHP1-ZF

Plasmid

#200911

Purpose

Express LHP1-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Publication

Wang et al Nat Plants. 2023 Mar 6. doi: 10.1038/s41

Insert

LHP1

Expression

Plant

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEG302-MSI1-ZF

Plasmid

#200912

Purpose

Express MSI1-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Publication

Wang et al Nat Plants. 2023 Mar 6. doi: 10.1038/s41

Insert

MSI1 (MSI1 Mustard Weed)

Expression

Plant

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEG302-JMJ14-ZF

Plasmid

#200913

Purpose

Express JMJ14-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Publication

Wang et al Nat Plants. 2023 Mar 6. doi: 10.1038/s41

Insert

JMJ14

Expression

Plant

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEG302-HD2A-ZF

Plasmid

#200915

Purpose

Express HD2A-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Publication

Wang et al Nat Plants. 2023 Mar 6. doi: 10.1038/s41

Insert

HD2A

Expression

Plant

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEG302-HD2C-ZF

Plasmid

#200917

Purpose

Express HD2C-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Publication

Wang et al Nat Plants. 2023 Mar 6. doi: 10.1038/s41

Insert

HD2C (HD2C Mustard Weed)

Expression

Plant

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEG302-CPL2-ZF

Plasmid

#200918

Purpose

Express CPL2-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Publication

Wang et al Nat Plants. 2023 Mar 6. doi: 10.1038/s41

Insert

CPL2 (CPL2 Mustard Weed)

Expression

Plant

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEG302-ELF7-ZF

Plasmid

#200920

Purpose

Express ELF7-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Publication

Wang et al Nat Plants. 2023 Mar 6. doi: 10.1038/s41

Insert

ELF7 (ELF7 Mustard Weed)

Expression

Plant

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMDC123-DMS3-ZF

Plasmid

#200921

Purpose

Express DMS3-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Publication

Wang et al Nat Plants. 2023 Mar 6. doi: 10.1038/s41

Insert

DMS3 (DMS3 Mustard Weed)

Expression

Plant

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMDC123-SUVH2-ZF

Plasmid

#200922

Purpose

Express SUVH2-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Publication

Wang et al Nat Plants. 2023 Mar 6. doi: 10.1038/s41

Insert

SUVH2 (SUVH2 Mustard Weed)

Expression

Plant

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMDC123-SUVH9-ZF

Plasmid

#200923

Purpose

Express SUVH9-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Publication

Wang et al Nat Plants. 2023 Mar 6. doi: 10.1038/s41

Insert

SUVH9 (SUVH9 Mustard Weed)

Expression

Plant

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMDC123-MORC1-ZF

Plasmid

#200924

Purpose

Express MORC1-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Publication

Wang et al Nat Plants. 2023 Mar 6. doi: 10.1038/s41

Insert

MORC1

Expression

Plant

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_EF-P49

Plasmid

#202176

Purpose

Level 0 plasmid containing a terminator sequence from gene 49202 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

P49 terminator

Use

Synthetic Biology

Mutation

None

Available since

Oct. 19, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-act1_full

Plasmid

#202172

Purpose

Level 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

act1 full terminator

Use

Synthetic Biology

Mutation

None

Available since

Sept. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-pyr_comp_sig_pep

Plasmid

#202132

Purpose

Level 0 plasmid containing a kozak consensus sequence and a pyrenoid compartment signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Pyrenoid compartment signal peptide

Use

Synthetic Biology

Mutation

None

Available since

Sept. 6, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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