We narrowed to 6,474 results for: poly

1. MSCV-N HPyV6

   Plasmid

   #37868

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   HPyV6 ER (HPyV6_gp4, HPyV6_gp5 Polyomavirus HPyV6)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   PKG

   Available since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

2. MSCV-N WU ER

   Plasmid

   #37867

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   WU ER (WUPyV_gp5, WUPyV_gp4 WU polyomavirus)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   PKG

   Available since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pLENTI EF1alpha FLAG/HA-LCOR

   Plasmid

   #114444

   Purpose

   Mammalian lentiviral expression vector containing N terminal FLAG/HA tagged human LCOR coding sequence

   Depositor

   Adrian Bracken

   Article

   Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

   Insert

   LCOR (LCOR Human)
   

   Use

   Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

   Plasmid

   #61364

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…

   Promoter

   CMV

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLPC-puromycin-binary-MDH1-3xFLAG-ME1- HA

   Plasmid

   #184549

   Purpose

   Retroviral vector to co-express human MDH1 with 3xFLAG tag and human ME1 with HA tag

   Depositor

   Gerardo Ferbeyre

   Article

   Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

   Inserts

   Malate dehydogenase 1 (MDH1 Human)
   Malic Enzyme 1 (ME1 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG and HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUltra-MDH1-ME1

   Plasmid

   #184465

   Purpose

   Lentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)

   Depositor

   Gerardo Ferbeyre

   Article

   Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

   Inserts

   Malate dehydrogenase 1 (MDH1 Human)
   Malic Enzyme 1 (ME1 Human)
   

   Use

   Lentiviral

   Tags

   fused to T2A

   Expression

   Mammalian

   Mutation

   deletion of stop codon

   Promoter

   UbC

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1_ spike_del19

   Plasmid

   #155297

   Purpose

   Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles

   Depositor

   Raffaele De Francesco

   Article

   Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2

   Insert

   SARS-Cov2 spike_deleted (S SARS-Cov2)
   

   Use

   Generation of sars-cov-2-pseudotyped lentiviral p…

   Tags

   Expression

   Mutation

   Deletion in C-term 19 aa of spike

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pLEW13

   Plasmid

   #24007

   Depositor

   George Cross

   Insert

   T7 RNA Polymerase
   

   Use

   Trypanosome expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAcGFP-IVT

   Plasmid

   #205145

   Purpose

   IVT template of AcGFP with MCS in frame and polyA

   Depositor

   Igor Ulitsky

   Insert

   AcGFP1
   

   Use

   In-vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEB-C5

    Plasmid

    #28213

    Purpose

    5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are expressed as a single poly-cistronic unit

    Depositor

    Linzhao Cheng

    Article

    Chou et al Cell Res. 2011 Jan 18. ():.

    Insert

    Oct4, Sox2, Klf4, c-Myc, Lin28 (Myc, Sox2, Pou5f1, Klf4, Lin28a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2FLAG

    Plasmid

    #16331

    Depositor

    Peter Klein

    Insert

    Flag tag
    

    Use

    Vertebrate expression

    Tags

    Flag

    Expression

    Mutation

    Flag tag inserted into pCS2+ polylinker

    Promoter

    Available since

    Dec. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAcGFP-stop-IVT

    Plasmid

    #205146

    Purpose

    IVT template of AcGFP stop codon between the ORF and MCS and polyA

    Depositor

    Igor Ulitsky

    Insert

    AcGFP1
    

    Use

    In-vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOpen-T4 PNK

    Plasmid

    #165520

    Purpose

    The most commonly used polynucleotide kinase.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    T4 PNK
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. SV40-MCS-Z1

    Plasmid

    #127639

    Purpose

    Empty backbone plasmid with SV40 promoter and SV40 polyA with Zeocin/Bleocin bacterial resistance

    Depositor

    Jordan Green

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 promoter

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFFLuc-IVT

    Plasmid

    #205147

    Purpose

    IVT template of FireFly luciferase stop codon between the ORF and MCS and polyA

    Depositor

    Igor Ulitsky

    Insert

    FireFly luciferase
    

    Use

    Luciferase; In-vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRLuc-IVT

    Plasmid

    #205148

    Purpose

    IVT template of Renilla luciferase stop codon between the ORF and MCS and polyA

    Depositor

    Igor Ulitsky

    Insert

    Renilla Luciferase
    

    Use

    Luciferase; In-vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAcRP67A prM/E YFV

    Plasmid

    #52883

    Purpose

    expression of Yellow Fever Virus VLPs in baculovirus

    Depositor

    Yorgo Modis

    Insert

    prM/E
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pOpen-TthRT

    Plasmid

    #165566

    Purpose

    DNA polymerase that efficiently reverse-transcribes RNA in the presence of MnCl2

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    Thermus thermophilus (Tth) RT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. Hsp68-CreER(TM) (DM#264)

    Plasmid

    #15028

    Depositor

    Douglas Melton

    Insert

    Cre-ER(TM)
    

    Use

    Cre/Lox; For making transgenic constructs

    Tags

    Hsp68 promoter and polyA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAB-LIC C

    Plasmid

    #62288

    Purpose

    Baculovirus expression vector for secretion with N-terminal Honeybee melittin signal and C-terminal 8xHis tag

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Baculovirus

    Tags

    8XHis tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only