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sgTAZ-R-1

Plasmid

#229448

Purpose

knockout of WWTR1 (TAZ)

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgTAZ-R-1 (WWTR1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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sgCDK1-ROSA

Plasmid

#229443

Purpose

knockout of CDK1

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgCDK1-ROSA (CDK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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sgMARK2-3-2

Plasmid

#229441

Purpose

knockout of MARK2 and MARK3

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgMARK2-3-2 (MARK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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sgMARK2-3-1

Plasmid

#229440

Purpose

knockout of MARK2 and MARK3

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgMARK2-3-1 (MARK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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sgMARK3-R-3

Plasmid

#229439

Purpose

knockout of MARK3

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgMARK3-R-3 (MARK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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sgMARK3-R-1

Plasmid

#229437

Purpose

knockout of MARK3

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgMARK3-R-1 (MARK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-YAP-5D

Plasmid

#229415

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

YAP-5D (YAP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

MARK2 phosphosites (S128,S163,S350,T354,T398) mut…

Promoter

EFS

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pOGS539_GLuc

Plasmid

#226718

Purpose

Plasmid enabling yeast-mediated expression and secretion of Gaussia luciferase (GLuc)

Depositor

Gautam Dantas

Article

Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

Insert

Gaussia Luciferase

Tags

HA tag and Mating factor alpha secretion signal

Expression

Yeast

Promoter

pTEF1

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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HA-YAP-5A

Plasmid

#229413

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

YAP-5A (YAP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

MARK2 phosphosites (S128,S163,S350,T354,T398) mut…

Promoter

EFS

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 IST1 L328D, L355A (1-366)

Plasmid

#193040

Purpose

Bacterial expression plasmid for full-length IST1 (1-366) with L328D and L355A mutations that diminish binding to MIT domains.

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

IST1 (IST1 Human)

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

L328D, L355A

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 IST1, residues 316-366, C-Cys

Plasmid

#191234

Purpose

Bacterial expression for IST1 residues 316-366, contains non-native C-terminal Cysteine for labeling

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

IST1 (IST1 Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 316-366, contains nonnative C-terminus f…

Promoter

T7

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 CHMP1A 140-196

Plasmid

#104593

Purpose

Expresses C-terminal 140-196 residues of CHMP1A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-1

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CHMP1A residues 140-196 (CHMP1A Human)

Tags

His-SUMO

Expression

Bacterial

Mutation

Contains nonnative Gly-Cys for fluorescent labeli…

Promoter

T7

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGECPL.BC10.MG

Plasmid

#223822

Purpose

Contains Cas9-editable barcode, marking guide (MG) for lineage tracing, Cre for Cas9 activation and loxP-mediated gene deletion, and Firefly Luciferase (FLuc) for live luminescence-based monitoring.

Depositor

Dawid Nowak

Article

Serio et al Cancer Discov. 2024 Jul 5. doi: 10.1158/

Insert

Ef1a-Driven Cre-P2A-FLuc

Use

CRISPR, Cre/Lox, Lentiviral, Luciferase, and Mous…

Tags

P2A

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pPU-huMDA5-miR30-L1221

Plasmid

#174223

Purpose

All-in-one huMDA5 rescue-control shRNA knockdown vector

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MDA5 (IFIH1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSD139A

Plasmid

#218129

Purpose

Hybrid circuit displaying hsCRBN-W384A,Y386A bait (P22 anchor) and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

CRBN (CRBN Human)
luxAB

Tags

scP22

Mutation

W384A, Y386A

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#3

Plasmid

#208389

Purpose

Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#3 gene.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgZBP1 (Zbp1 Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

WT

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF3088

Plasmid

#144564

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

E4F1 (E4F1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-EGFP-N3-CYB5R3 K214R

Plasmid

#204570

Purpose

Expresses mCherry-EGFP-tagged CYB5R3 K214R in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

CYB5R3 (CYB5R3 Human)

Expression

Mammalian

Mutation

K214R

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-EGFP-N3-CYB5R3

Plasmid

#204569

Purpose

Expresses mCherry-EGFP-tagged CYB5R3 in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

CYB5R3 (CYB5R3 Human)

Expression

Mammalian

Mutation

none

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 IST1, residues 315-360, no Cys

Plasmid

#180643

Purpose

Bacterial expression of IST1 C-terminal tail, residues 315-360. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-99.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

IST1 (IST1 Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

residues 315-360

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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