We narrowed to 9,370 results for: BLI

1. pHH0103 APBB2 WW domain

   Plasmid

   #104257

   Purpose

   Bacterial expression of WW domain from APBB2

   Depositor

   Marius Sudol, Sachdev Sidhu

   Insert

   APBB2 WW domain (APBB2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTF276 (pFA6a-PSc-6xGly-2xStrep-His3MX)

   Plasmid

   #44064

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   2xStrep tag, 6xGly linker, ADH1 transcription ter…

   Available Since

   April 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUASp YFP Rholike DN

   Plasmid

   #52240

   Purpose

   Drosophila expression of YFP-Rholike dominant negative mutant

   Depositor

   Matthew Scott

   Insert

   Rholike (RhoL Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   T25N; DN mutation

   Promoter

   GAL4

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pAc.1B-EGFP-DmHrb98

   Plasmid

   #66595

   Purpose

   Expresses EGFP-tagged Hrb98 in Drosophila cells

   Depositor

   Elisa Izaurralde

   Insert

   Hrb98 (Hrb98DE Fly)
   

   Use

   Drosophila expression

   Tags

   EGFP

   Promoter

   Actin 5C

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCRII-Topo Lhx2

   Plasmid

   #45873

   Depositor

   Jeffrey Macklis

   Insert

   Lhx2 in situ probe (Lhx2 Mouse)
   

   Use

   In situ

   Mutation

   contains bp# 1809-2217 of NM_010710.3

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pJK693

   Plasmid

   #72382

   Purpose

   Produces Escherichia coli cysteine desulfurase IscS with Ala260>Thr mutation, N-terminal His6-tag (H6EcIscS-A260T)

   Depositor

   T. J. Kappock

   Insert

   cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent (iscS Escherichia coli str. K-12 substr. MG1655)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Mutated alanine-260 to threonine

   Promoter

   T7

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLV-Enh eGFP Reporter-muEDC-Int3

   Plasmid

   #59288

   Purpose

   Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intronic region of the mouse Epidermal Differentiation Complex.

   Depositor

   Benjamin Yu

   Insert

   mFlg int-Intron 1
   

   Use

   Lentiviral

   Promoter

   Mu Hsp68 minimal promoter

   Available Since

   Dec. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

8. p14-9VNA5

   Plasmid

   #50931

   Purpose

   Expresses human SRP14A5 and SRP9 proteins as a fusion protein in mammalian cells.

   Depositor

   Katharina Strub

   Insert

   SRP14 and SRP9 (SRP14, SRP9 Human)
   

   Tags

   VN tag and flag tag

   Expression

   Mammalian

   Mutation

   Amino acids 96-100 of SRP14 changed to alanine

   Promoter

   CMV

   Available Since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pPyCAG-Nanog(Y136A)-IP

   Plasmid

   #62995

   Purpose

   Expresses Mouse Nanog Y136A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog Y136A Mutant (Nanog Mouse)
   

   Expression

   Mammalian

   Mutation

   Changed Tyrosine 136 (137 AA in Mouse Protein) to…

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CSNK1G2

    Plasmid

    #39150

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CSNK1G2 (CSNK1G2 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pZC05 (pFA6a-TEV-6xGly-PrtA-KanMX)

    Plasmid

    #44096

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    6xGly linker, ADH1 transcription terminator, KanM…

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIRES-Neo-HA-cdc25C1

    Plasmid

    #40966

    Depositor

    Olivier Gavet

    Insert

    cdc25C5 (CDC25C Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

13. Foxd3 CreERT2 RMCE Insertion Vector

    Plasmid

    #37272

    Depositor

    Patricia Labosky

    Insert

    Foxd3 (Foxd3 Mouse)
    

    Use

    Mouse Targeting; Insertion vector

    Available Since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pCVL RipTAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers

    Plasmid

    #50626

    Purpose

    pRipTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.

    Depositor

    Andrew Scharenberg

    Type

    Empty backbone

    Use

    Lentiviral and TALEN

    Expression

    Mammalian

    Available Since

    May 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SLC9A3R2

    Plasmid

    #38948

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SLC9A3R2 (NHERF2 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

16. PX379 Legionella pneumophila str. Paris Cas9

    Plasmid

    #68316

    Purpose

    Legionella pneumophila str. Paris Cas9

    Depositor

    Feng Zhang

    Insert

    LpCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PX391 Sphaerochaeta globus str. Buddy Cas9

    Plasmid

    #68328

    Purpose

    Sphaerochaeta globus str. Buddy Cas9

    Depositor

    Feng Zhang

    Insert

    SgCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZC07 (pFA6a-TEV-6xGly-PrtA-HphMX)

    Plasmid

    #44084

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    6xGly linker, ADH1 transcription terminator, HphM…

    Available Since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUASP Flag Rab32 WT

    Plasmid

    #44833

    Depositor

    Matthew Scott

    Insert

    Rab32 (Rab32 Fly)
    

    Use

    Drosophila

    Tags

    Flag

    Expression

    Insect

    Available Since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. ENOSF1

    Plasmid

    #39104

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ENOSF1 (ENOSF1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only