We narrowed to 13,726 results for: Cas9

1. pmCherry-U6-CLIC3

   Plasmid

   #140583

   Purpose

   Expresses a gRNA and mCherry in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   gRNA CLIC3
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-pegRNA-HEK3+24

   Plasmid

   #140577

   Purpose

   Expresses a pegRNA in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   pegRNA HEK3 +24
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. peft-3::cas-9::tbb-2 3'UTR

   Plasmid

   #48960

   Purpose

   this plasmid contains the codon optimized gene encoding Cas9 for C. elegans.

   Depositor

   Mario de Bono

   Article

   Chen et al Nucleic Acids Res. 2013 Sep 5.

   Insert

   cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   eef-1A.1 (eft-3)

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCR1003

   Plasmid

   #71821

   Purpose

   Express Streptococcus pyogenes dCas9 (D10A, H840A)

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Insert

   T7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOsU3-esgRNA

   Plasmid

   #115629

   Purpose

   expression esgRNA in rice protoplasts

   Depositor

   Caixia Gao

   Article

   Li et al Genome Biol. 2018 May 29;19(1):59. doi:

   Insert

   OsU3p-esgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJin-278

   Plasmid

   #125714

   Purpose

   ABA-inducible split T7 GFP vector using RNAPN d5-19

   Depositor

   Bryan Dickinson

   Article

   Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

   Insert

   PT7-GFP mRNA expression, PYL-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-ABI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG ERT2-Cre-ERT2 lox

   Plasmid

   #183447

   Purpose

   CreOFF tamoxifen-inducible Cre

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   ERT2-Cre-ERT2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCfB8792

   Plasmid

   #126910

   Purpose

   Template for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 gene

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   ADE2 gRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. BCJJ339

   Plasmid

   #117501

   Purpose

   RPS5a:Cas9:E9 Golden Gate Level 1 Position 2

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:GCAA:RPS5a:Cas9-3(intron):E9:ACTA:BpiI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtRPS5a

   Available since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNeurog2-Luciferase reporter

    Plasmid

    #64126

    Purpose

    Photoactivatable transcription system. Luciferase reporter under the control of the Neurog2 promoter.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    Neurog2 promoter
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Neurog2

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19, containing H2-Kb c57/BL6 WK9

    Plasmid

    #102641

    Purpose

    Encodes sequence of the murine H2-Kb allele

    Depositor

    Sai Reddy

    Article

    Kelton et al Sci Rep. 2017 Apr 4;7:45775. doi: 10.103

    Insert

    H2-Kd Balb/c
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmCherry-U6-EMX1

    Plasmid

    #140581

    Purpose

    Expresses a gRNA and mCherry in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    gRNA EMX1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. AP582-2

    Plasmid

    #70049

    Purpose

    Expresses Ollas::mCherry::linker::H2B::V5 in bacteria

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

    Insert

    mCherry::linker::H2B
    

    Use

    Tags

    Ollas and V5

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. PM-SP!TB

    Plasmid

    #48650

    Purpose

    Bacterial SP crRNA expression: targets SP to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Bacterial SP crRNA to prototspacer B
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    J23100 promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCR1055

    Plasmid

    #71823

    Purpose

    Express Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLS

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

    Insert

    T7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ147

    Plasmid

    #69299

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 7 gRNAs

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJA50

    Plasmid

    #59931

    Purpose

    gRNA for cleavage at unc-58(e665) locus in C elegans

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    unc-58(e665) gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. BCJJ358

    Plasmid

    #117505

    Purpose

    RPS5a:Cas9:E9 Golden Gate Level 1 Position 2 reverse

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:tagt:RPS5a:Cas9_4:E9:ttgc:BpiI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtRPS5a

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. XX>sgRNA F+E

    Plasmid

    #87543

    Purpose

    Template 2 (of 2) for U6>sgRNA cassette OSO-PCR

    Depositor

    Lionel Christiaen

    Article

    Gandhi et al Dev Biol. 2017 May 1;425(1):8-20. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lentiGuide-Puro-Chen-tracrRNA_2xBsmBI

    Plasmid

    #196710

    Purpose

    Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice

    Depositor

    Bernhard Schmierer

    Article

    Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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