We narrowed to 5,570 results for: SPI

1. pRSF_his6_sumo_SARS_CoV2_NSP14_V290A

   Plasmid

   #213522

   Purpose

   Expresses NSP14 of SARS-CoV-2 with a single point mutation V290A

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   changed V290 to A

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSF_his6_sumo_SARS_CoV2_NSP14_N306K

   Plasmid

   #213523

   Purpose

   Expresses NSP14 of SARS-CoV-2 with a single point mutation N306K

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   SARS-CoV2-NSP14 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   changed N306 to K

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRSF_his6_sumo_SARS_CoV2_NSP14_P335S

   Plasmid

   #213524

   Purpose

   Expresses NSP14 of SARS-CoV-2 with a single point mutation P335S

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   changed P335 to S

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRSF_his6_sumo_SARS_CoV2_NSP14_P335H

   Plasmid

   #213525

   Purpose

   Expresses NSP14 of SARS-CoV-2 with a single point mutation P335H

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   changed P335 to H

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRSF_his6_sumo_SARS_CoV2_NSP14_H424R

   Plasmid

   #213526

   Purpose

   Expresses NSP14 of SARS-CoV-2 with a single point mutation H424R

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   changed H424 to R

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CIRTS-YTHDF2-Malat1

   Plasmid

   #216858

   Purpose

   CIRTS RNA targeting system for targeted knockdown of m6A-containing Malat1 at the synapse. CIRTS-YTHDF2-Calm3 and Malat1 gRNA is expressed under human Syn1 and U6 promoter, respectively.

   Depositor

   Timothy Bredy

   Article

   Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100

   Insert

   CIRTS-YTHDF2-Calm3-U6-Malat1 gRNA
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Syn1, U6

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. CIRTS-FTO-Malat1

   Plasmid

   #216860

   Purpose

   CIRTS RNA targeting system for targeted removal of m6A on Malat1 at the synapse. CIRTS-FTO-Calm3 and Malat1 gRNA is expressed under human Syn1 and U6 promoter, respectively.

   Depositor

   Timothy Bredy

   Article

   Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100

   Insert

   CIRTS-FTO-Calm3-U6-Malat1 gRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Syn1, U6

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpyTag-RBD Omicron XBB.1.5

   Plasmid

   #214724

   Purpose

   Expresses SARS-CoV-2 Omicron XBB.1.5 RBD (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

   Depositor

   Mark Howarth

   Article

   Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103

   Insert

   Receptor-Binding Domain (S SARS-CoV-2)
   

   Use

   Tags

   SpyTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVRC8400-SARS-CoV-2-RBD-BJ.1-AVI

   Plasmid

   #196617

   Purpose

   Expresses SARS-CoV-2 RBD protein from the BJ.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

   Insert

   SARS-CoV-2-RBD-BJ.1
   

   Use

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   G339H, R346T, L368I, S371F, S373P, S375F, T376A, …

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV.CBA.YFP.miR-E_shAtxn1

    Plasmid

    #180387

    Purpose

    Producing AAV that encodes mouse Atxn1 shRNA with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    shAtxn1 (Atxn1 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLD-puro-Cc-PARK7V51G-VA

    Plasmid

    #115183

    Purpose

    Lentiviral transduction and expression of PARK7V51G into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    PARK7V51G (PARK7 Human)
    

    Use

    Lentiviral

    Tags

    Versatile affinity tag (2XStreptactin II-6XHis-3X…

    Expression

    Mutation

    p.V51G

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLD-puro-Cc-PARK7C53A-VA

    Plasmid

    #115184

    Purpose

    Lentiviral transduction and expression of PARK7C53A into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    PARK7C53A (PARK7 Human)
    

    Use

    Lentiviral

    Tags

    Versatile affinity tag (2XStreptactin II-6XHis-3X…

    Expression

    Mutation

    p.C53A

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLD-puro-Cc-PARK7H126A-VA

    Plasmid

    #115185

    Purpose

    Lentiviral transduction and expression of PARK7H126A into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    PARK7H126A (PARK7 Human)
    

    Use

    Lentiviral

    Tags

    Versatile affinity tag (2XStreptactin II-6XHis-3X…

    Expression

    Mutation

    p.H126A

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLD-puro-Cc-PARK7E163K-VA

    Plasmid

    #115186

    Purpose

    Lentiviral transduction and expression of PARK7E163K into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    PARK7E163K (PARK7 Human)
    

    Use

    Lentiviral

    Tags

    Versatile affinity tag (2XStreptactin II-6XHis-3X…

    Expression

    Mutation

    p.E163K

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPHAGE-C20orf24- C20orf24-3’UTR-VAR

    Plasmid

    #128509

    Purpose

    Lentiviral constitutive expression of C20orf24 under control of its native 3'UTR of human C20orf24 with variant from COX deficiency patient.

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    RAB5IF (RAB5IF Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOTTC1079 - pAAV TH gRNA A EF1a EGFP

    Plasmid

    #113159

    Purpose

    An AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat TH
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOTTC1088 - pAAV TH gRNA A+B pair EF1a EGFP

    Plasmid

    #113155

    Purpose

    An AAV vector that expresses guide RNAs targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat TH
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOTTC1388 - pAAV MANF gRNA A+B EF1a EGFP

    Plasmid

    #113157

    Purpose

    An AAV vector that expresses guide RNAs targeting rat MANF and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat MANF
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. His6-TEV-CRBN-midi

    Plasmid

    #215330

    Purpose

    CRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader design

    Depositor

    Alessio Ciulli

    Article

    Kroupova et al Nat Commun. 2024 Oct 15;15(1):8885. doi:

    Insert

    CRBN (CRBN Human)
    

    Use

    Tags

    6xHis and TEV

    Expression

    Bacterial

    Mutation

    contains amino acids 41-187 followed by a Gly-Ser…

    Promoter

    lacI

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-SpCas9(KWRQLC)-P2A-EGFP (RAS2864)

    Plasmid

    #223087

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KWRQLC)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(KWRQLC)=D1135K, S1136W, G1218R, E1219Q, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge