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We narrowed to 5,790 results for: SPI

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Showing: 5021 - 5040 of 5790 results

  6. pcDNA3-sACE2v5

    Plasmid
    #145152
    Purpose
    Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 5
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145152-plasmid-map-sequence-id-292590

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  10. pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

    Plasmid
    #185910
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8e-nSpCas9-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A
    Promoter
    CMV and T7
    Available Since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185910-plasmid-map-sequence-id-363739

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  11. pDEST-N2H-N1

    Plasmid
    #125547
    Purpose
    Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment1
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment1 attached to n-terminus of Gatew…
    Expression
    Bacterial and Mammalian
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125547-plasmid-map-sequence-id-254706

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  12. pDEST-N2H-C1

    Plasmid
    #125549
    Purpose
    Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment1
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment1 attached to c-terminus of Gatew…
    Expression
    Mammalian and Yeast
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125549-plasmid-map-sequence-id-254837

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  13. pDEST-N2H-C2

    Plasmid
    #125559
    Purpose
    Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment2
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment2 attached to c-terminus of Gatew…
    Expression
    Mammalian and Yeast
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125559-plasmid-map-sequence-id-254701

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  14. pDEST-N2H-N2

    Plasmid
    #125548
    Purpose
    Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment2
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment2 attached to n-terminus of Gatew…
    Expression
    Mammalian and Yeast
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125548-plasmid-map-sequence-id-254836

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  15. LV-AR-C563A-C596A

    Plasmid
    #171220
    Purpose
    Lenti-viral expression of human androgen receptor DNA-binding mutant: mutations of one essential cysteine on each of the two zinc fingers.
    Depositor
    Steven Balk
    Article
    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:
    Insert
    AR-C563A-C596A (AR Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-C563A-C596A
    Promoter
    EF-1a
    Available Since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    171220-plasmid-map-sequence-id-342449

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  16. LV-AR-R599A-N600A

    Plasmid
    #171221
    Purpose
    Lenti-viral expression of human androgen receptor DNA-binding mutant: R599A-N600A, mutations in the D-box of the Zinc finger 2 that mediates AR dimerization.
    Depositor
    Steven Balk
    Article
    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:
    Insert
    AR-R599A-N600A (AR Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-R599A-N600A
    Promoter
    EF-1a
    Available Since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    171221-plasmid-map-sequence-id-342450

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  18. PB53x EF1-Dendra2-RPB1Amr

    Plasmid
    #81228
    Purpose
    expresses Dendra2-Rpb1 fusion in mammalian cells. Possible to insert into the genome using the Piggibac system
    Depositor
    Timothée Lionnet
    Article
    Cho et al Elife. 2016 May 3;5. pii: e13617. doi: 1
    Insert
    Dendra2 - Rpb1 (alpha amanitin resistant) (POLR2A Human)
    Tags
    Dendra2
    Expression
    Mammalian
    Mutation
    N792D alpha amanitin resistance mutation (Bartolo…
    Promoter
    EF1a
    Available Since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    81228-plasmid-map-sequence-id-394051

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  20. pCENPTΔC-dCas9-3xGFP

    Plasmid
    #198325
    Purpose
    Expresses CENPTΔC-dCas9-3xGFP protein in mammalian cells. When coupled with a guide RNA against a high repeat target locus, this can be applied to seed ectopic kinetochores at the target locus
    Depositor
    Sarah McClelland
    Article
    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:
    Insert
    CENPTΔC (CENPT Human)
    Use
    Lentiviral
    Tags
    EGFP x 3 and dCas9
    Expression
    Mammalian
    Mutation
    truncation - encodes only amino acids 1-375
    Promoter
    CMV-TetO
    Available Since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198325-plasmid-map-sequence-id-390299

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Showing: 5021 - 5040 of 5790 results