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pDonor_e-attP-H1-g3-Ef1a-Puro-P2A-mCherry

Plasmid

#247160

Purpose

Engineered e-attP donor plasmid expressing puromycin resistance and mCherry with donor binding sgRNA H1-g3

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Puromycin resistance, mCherry

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-PKCb1-mEGFP-N1

Plasmid

#112265

Purpose

ITRACKbeta/IDOCKSbeta Donor construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

protein kinase c beta (Prkcb Mouse)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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USP19 (C506A)-FLAG/pRK5

Plasmid

#36307

Depositor

Xiaolu Yang

Article

Mei et al J Biol Chem. 2011 Oct 14;286(41):35380-7

Insert

ubiquitin specific peptidase 19 (USP19 Human)

Tags

FLAG

Expression

Mammalian

Mutation

C506A

Promoter

CMV

Available Since

May 11, 2012

Availability

Academic Institutions and Nonprofits only
pET19b Adrm1

Plasmid

#19423

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

adhesion regulating molecule 1 (ADRM1 Human)

Tags

His

Expression

Bacterial

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3 HA Sam68 G178E

Plasmid

#17689

Depositor

David Shalloway

Article

Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

Insert

Sam68 (Khdrbs1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

G178E

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pUB23-P-beta-gal

Plasmid

#8817

Depositor

Alexander Varshavsky

Article

Bachmair et al Science 1986 Oct 10;234(4773):179-86.

Insert

beta-gal

Tags

Ubiquitin

Expression

Yeast

Mutation

P (proline) at amino terminus of beta-gal

Available Since

Dec. 28, 2005

Availability

Academic Institutions and Nonprofits only
pUB23-R-beta-gal

Plasmid

#8818

Depositor

Alexander Varshavsky

Article

Bachmair et al Science 1986 Oct 10;234(4773):179-86.

Insert

beta-gal

Tags

Ubiquitin

Expression

Yeast

Mutation

R (arginine) at amino terminus of beta-gal

Available Since

Dec. 28, 2005

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag HK3

Plasmid

#20414

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

HK3 (HK3 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag CDK4

Plasmid

#20448

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CDK4 (CDK4 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
PKG I alpha dNPS

Plasmid

#16391

Depositor

Bernard Weinstein

Article

Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Insert

PKG1 alpha (PRKG1 Human)

Tags

HA

Expression

Mammalian

Mutation

N-terminal pseudosubstrate domain deletion: delet…

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pSpCas9_T2A_mCherry sgBFP

Plasmid

#192929

Purpose

All-in-one plasmid for cutting BFP region in DSB Spectrum V2

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Tags

T2A mCherry

Expression

Mammalian

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAF0035 Ef1a-Nm60-2a-EGFP

Plasmid

#193464

Purpose

Expression of Nm60 recombinase from Ef1a promoter

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Nm60

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLC-mCherry-MLKL

Plasmid

#75167

Purpose

LentiCRISPR-mCherry with sgRNA targeting human MLKL

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

MLKL sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCY 3110-01

Plasmid

#36236

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

mEos2

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-mEos2-kanMX

Available Since

June 19, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pet42c(+)-GST-USP13

Plasmid

#61749

Purpose

express N-terminally GST tagged human USP13

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP13 (USP13 Human)

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only
pSS2 (hS100B)

Plasmid

#26774

Depositor

Gary Shaw

Article

Smith et al Biochemistry. 1996 Jul 9. 35(27):8805-14

Insert

S100B (S100B Human)

Expression

Bacterial

Available Since

Nov. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pDEST17-hOPTN

Plasmid

#23053

Depositor

Jon Ashwell

Article

Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43.

Insert

optineurin (OPTN Human)

Tags

His

Expression

Bacterial

Mutation

N/A

Available Since

May 3, 2010

Availability

Academic Institutions and Nonprofits only

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pBabe puro MTH1

Plasmid

#21295

Depositor

Bob Weinberg

Article

Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

MTH1 (NUDT1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
pLIA-E p27 (CC#620)

Plasmid

#15191

Depositor

Connie Cepko

Article

Dyer et al J Neurosci. 2001 Jun 15. 21(12):4259-71.

Insert

p27 (Cdkn1b Mouse)

Use

Retroviral

Tags

Flag and His

Expression

Mammalian

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
p4949 pLPCX-His-Xpress NLS hBrd4 CTD

Plasmid

#14459

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 CTD (BRD4 Human)

Use

Retroviral

Tags

SV40 NLS

Expression

Mammalian

Mutation

amino acids 1047-1362 of Brd4 (the CTD). Function…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only