We narrowed to 5,233 results for: puc
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Plasmid#185896PurposeIn vivo gene amplification (HapAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PSk.GAL2>Y.FAST-EVBR1.2A>TRPL41B-PPDA1>RPL25(partial;Arm2)
ExpressionYeastMutationNoneAvailable SinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTR-377 tNGFR-P2A-CD5
Plasmid#186071PurposeKnockin of truncated NGFR to target geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTR-455 tNGFR-P2A-WAS
Plasmid#186082PurposeKnockin of truncated NGFR to target geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDB-hPER2-SNAP-CD4-bla
Plasmid#179452PurposeDonor vector to knock in SNAPtag C-terminal to human PER2 geneDepositorInsertSnapTag
UseCRISPRTagsHis/FlagPromoterNoneAvailable SinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
RP51A-No5'ss-NoBS-NoPseudoBS
Plasmid#100987PurposeRP51A with T7 promoter in HindIII/EcoRI sites of pUC18. No 5'ss, No BS, No pseudo BSDepositorInsertRP51A (RPS17A Budding Yeast)
ExpressionBacterial and YeastMutation5'ss mut to C/ATACCT BS mut to GTTAGTG, pseu…Available SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA
Plasmid#173211PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
UseCRISPR; Prime editing (pe3)ExpressionMammalianPromoterTandem U6 promotersAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
PW124-LMNA 5'HA-TriTag (mCherry)-3'HA (HDR donor)
Plasmid#164047PurposeCRISPR donor plasmid to insert TriTag (mCherry) into the N-terminus of human LMNA geneDepositorInsertmCherry-TriTag
UseCRISPRExpressionMammalianAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)5
Plasmid#127523PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 5 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 5 attachment sites.
ExpressionPlantAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)7
Plasmid#127524PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 7 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 7 attachment sites.
ExpressionPlantAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)9
Plasmid#127525PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 9 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 9 attachment sites.
ExpressionPlantAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)2
Plasmid#127521PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 2 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 2 attachment sites.
ExpressionPlantAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZNF778-donor
Plasmid#112379PurposeCRISPR donor plasmid to tag human transcription factor ZNF778 with GFPDepositorInsertZNF778 homology arms flanking EGFP-IRES-Neo cassette (ZNF778 Human)
UseCRISPRMutationrs4785627,rs28415940,rs9921361,rs28638280,rs60437…Available SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF696-donor
Plasmid#113806PurposeCRISPR donor plasmid to tag human transcription factor ZNF696 with GFPDepositorInsertZNF696 homology arms flanking EGFP-IRES-Neo cassette (ZNF696 Human)
UseCRISPRMutationrs7386259, rs3750217, rs7387702Available SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTOX2-donor
Plasmid#113819PurposeCRISPR donor plasmid to tag human transcription factor TOX2 with GFPDepositorAvailable SinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEHF-donor
Plasmid#113817PurposeCRISPR donor plasmid to tag human transcription factor EHF with GFPDepositorInsertEHF homology arms flanking EGFP-IRES-Neo cassette (EHF Human)
UseCRISPRMutationrs3818514, rs11032804, hg38:11:34659087:G>AAvailable SinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTCFL5-donor
Plasmid#113808PurposeCRISPR donor plasmid to tag human transcription factor TCFL5 with GFPDepositorInsertTCFL5 homology arms flanking EGFP-IRES-Neo cassette (TCFL5 Human)
UseCRISPRMutationhg38:20:62842215:T>AAvailable SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF250-donor
Plasmid#112383PurposeCRISPR donor plasmid to tag human transcription factor ZNF250 with GFPDepositorInsertZNF250 homology arms flanking EGFP-IRES-Neo cassette (ZNF250 Human)
UseCRISPRMutationhg38:8:144882271:C>G,hg38:8:144882273:T>CAvailable SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pE2F5-donor
Plasmid#112351PurposeCRISPR donor plasmid to tag human transcription factor E2F5 with GFPDepositorInsertE2F5 homology arms flanking EGFP-IRES-Neo cassette (E2F5 Human)
UseCRISPRMutationhg38:8:85213859:T>C, hg38:8:85213860:T>G, h…Available SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF23-donor
Plasmid#112325PurposeCRISPR donor plasmid to tag human transcription factor ZNF23 with GFPDepositorInsertZNF23 homology arms flanking EGFP-IRES-Neo cassette (ZNF23 Human)
UseCRISPRMutationrs1804984Available SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF764-donor
Plasmid#112298PurposeCRISPR donor plasmid to tag human transcription factor ZNF764 with GFPDepositorInsertZNF764 homology arms flanking EGFP-IRES-Neo cassette (ZNF764 Human)
UseCRISPRMutationhg38:16:30555233:G>A, hg38:16:30555234:C>G,…Available SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only