We narrowed to 22,191 results for: his

1. GvpA-CaM

   Plasmid

   #223555

   Purpose

   Full length gas vesicle coat protein A derived from H. salinarum (GvpA) fused to calmodulin.

   Depositor

   Jean Gariepy

   Article

   Fernando et al ACS Pharmacol Transl Sci. 2024 Jun 3;7(6

   Insert

   pET300-GvpACaM
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET22b Ag43 160N 6H mCherry-R5 AmpR

   Plasmid

   #225141

   Purpose

   Recombinant Ag43 protein fused to silaffin-R5 and mCherry. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.

   Depositor

   Urartu Seker

   Article

   Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.

   Insert

   Ag43 160N, mCherry
   

   Use

   Tags

   Ag43 protein fused to silaffin-R5 and mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pVF18

   Plasmid

   #229456

   Purpose

   Expresses IraD (aa17-130) with His-tag

   Depositor

   Alexandra Deaconescu

   Article

   Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

   Insert

   IraD (aa 17-130 only)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   aa 17-130 only

   Promoter

   T7

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLei-sfGFP-D134*

   Plasmid

   #191110

   Purpose

   An E. coli sfGFP reporter with one TAG at position 134

   Depositor

   Han Xiao

   Article

   Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

   Insert

   superfolder green fluorescence protein
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   changing D134 to TAG

   Promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLei-sfGFP-V2*D134*

   Plasmid

   #191111

   Purpose

   An E. coli sfGFP reporter with two TAG at positions 2 & 134

   Depositor

   Han Xiao

   Article

   Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

   Insert

   superfolder green fluorescence protein
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   changing V2 & D134 to TAG

   Promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-Cas9-FCPF

   Plasmid

   #220132

   Purpose

   Expresses Cas9-FCPF in E.coli

   Depositor

   Xinlai Cheng

   Article

   Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

   Insert

   Cas9-FCPF (cas9 S. pyogenes)
   

   Use

   Tags

   His and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET50delCT_Vipp1_dL10Ala

   Plasmid

   #223817

   Purpose

   Bacterial expression of Vipp1_dL10Ala with C-terminal 6x His-Tag and 3C cleavage site

   Depositor

   Carsten Sachse

   Article

   Junglas et al Nat Struct Mol Biol. 2025 Mar;32(3):555-

   Insert

   Vipp1
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   replacement of 10 aa in Hinge 2 with Ala

   Promoter

   Available Since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-28a (+)-Gal4-p53

   Plasmid

   #171076

   Purpose

   Expresses Gal4-p53 fusion protein in bacteria

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Insert

   Gal4 DBD (NEWENTRY Budding Yeast)
   

   Use

   Tags

   His and p53(1-85)

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pColdI-GST-mGTSF2

   Plasmid

   #215714

   Purpose

   Bacterial expression of GST-tagged GTSF2

   Depositor

   Phillip Zamore

   Article

   Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

   Insert

   GTSF2 (Gtsf2 Mouse)
   

   Use

   Tags

   6x His and GST

   Expression

   Bacterial

   Mutation

   Promoter

   CspA

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGP-CMV-jGCaMP8f L27A

    Plasmid

    #204140

    Purpose

    Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with L27A mutation

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f L27A
    

    Use

    Tags

    N-terminal His tag

    Expression

    Mammalian

    Mutation

    Leucine 27 changed to Alanine

    Promoter

    CMV

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pQE60 R196A wgMDH

    Plasmid

    #204271

    Purpose

    Bacterial expression of R196A wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R196A

    Promoter

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pQE60 R196E wgMDH

    Plasmid

    #204272

    Purpose

    Bacterial expression of R196E wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R196E

    Promoter

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pQE60 R130Q wgMDH

    Plasmid

    #204268

    Purpose

    Bacterial expression of R130Q wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R130Q

    Promoter

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQE60 M66L wgMDH

    Plasmid

    #204247

    Purpose

    Bacterial expression of M66L wgMDH with IPTG induction - Subunit interface mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    M66L

    Promoter

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pQE60 M91Q wgMDH

    Plasmid

    #204250

    Purpose

    Bacterial expression of M91Q wgMDH with IPTG induction - Subunit interface mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    M91Q

    Promoter

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pQE60 D87L wgMDH

    Plasmid

    #204248

    Purpose

    Bacterial expression of D87L wgMDH with IPTG induction - Subunit interface mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    D87L

    Promoter

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBAD-P19-NP

    Plasmid

    #196608

    Purpose

    siRNA vector expressing TMV P19 and a 425bp-long NP sequence as inverted repeat

    Depositor

    Franco Lucchini

    Article

    Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

    Insert

    p19 + Influenza virus NP inverted repeat
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBEL1936

    Plasmid

    #195692

    Purpose

    Complementation of MlaF-MlaE-MlaDdelta153-183-MlaC-MlaB

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaFEDCB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    MlaF-MlaE-MlaDdelta153-183-MlaC-MlaB

    Promoter

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBEL1935

    Plasmid

    #195693

    Purpose

    Complementation of MlaF-MlaE-MlaDdelta173-183-MlaC-MlaB

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaFEDCB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    MlaF-MlaE-MlaDdelta173-183-MlaC-MlaB

    Promoter

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBEL1953

    Plasmid

    #195694

    Purpose

    Complementation of MlaF-MlaE-MlaD(F118A/L123A)-MlaC-MlaB

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaFEDCB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    MlaF-MlaE-MlaD(F118A/L123A)-MlaC-MlaB

    Promoter

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBEL1933

    Plasmid

    #195695

    Purpose

    Complementation of MlaF-MlaE-MlaD(F118A)-MlaC-MlaB

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaFEDCB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    MlaF-MlaE-MlaD(F118A)-MlaC-MlaB

    Promoter

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBEL1934

    Plasmid

    #195696

    Purpose

    Complementation of MlaF-MlaE-MlaD(L123A)-MlaC-MlaB

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaFEDCB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    MlaF-MlaE-MlaD(L123A)-MlaC-MlaB

    Promoter

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBEL2766

    Plasmid

    #196028

    Purpose

    Complementation of MlaF-MlaE-MlaDdelta141-183-MlaC-MlaB

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaFEDCB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    MlaF-MlaE-MlaDdelta141-183-MlaC-MlaB

    Promoter

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFR-mScarletI-antiFRET-P2A-GCaMP3

    Plasmid

    #191460

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) GCaMP3 to mScarlet-I fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    GCaMP3
    

    Use

    Tags

    His-mScarletI-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFR-mTq2-antiFRET-P2A-K-GECO1

    Plasmid

    #191469

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) K-GECO1 to mTurquoise2 fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    K-GECO1
    

    Use

    Tags

    His-mTurquoise2-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFR-mTq2-antiFRET-P2A-R-GECO1

    Plasmid

    #191471

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) R-GECO1 to mTurquoise2 fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    R-GECO1
    

    Use

    Tags

    His-mTurquoise2-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFR-mTq2-antiFRET-P2A-CH-GECO2.1

    Plasmid

    #191470

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) CH-GECO2.1 to mTurquoise2 fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    CH-GECO2.1
    

    Use

    Tags

    His-mTurquoise2-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFR-mScarletI-antiFRET-P2A-jGCaMP7c

    Plasmid

    #191461

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) jGCaMP7c to mScarlet-I fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    jGCaMP7c
    

    Use

    Tags

    His-mScarletI-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-PLS-KP7_rpoA-U

    Plasmid

    #190974

    Purpose

    Test the U-to-C editing activity of KP7 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP7
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PM18033-PLS-KP6-E67A_rpoA-C

    Plasmid

    #190992

    Purpose

    Test the C-to-U editing activity of KP6:E67A on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    DYW-KP:E67A

    Promoter

    CMV

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET-PLS-KP2-E67A_rpoA-U

    Plasmid

    #190959

    Purpose

    Test the U-to-C editing activity of KP2:E67A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:E67A

    Promoter

    T7

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET-PLS-KP6-A66S_rpoA-C

    Plasmid

    #190968

    Purpose

    Test the C-to-U editing activity of KP6:A66S on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:A66S

    Promoter

    T7

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PM18033-PLS-KP6-A66S_rpoA-C

    Plasmid

    #190988

    Purpose

    Test the C-to-U editing activity of KP6:A66S on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    DYW-KP:A66S

    Promoter

    CMV

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PM18033-PLS-KP5_rpoA-U

    Plasmid

    #190985

    Purpose

    Test the U-to-C editing activity of KP5 on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP5
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET-PLS-KP1_rpoA-U

    Plasmid

    #190955

    Purpose

    Test the U-to-C editing activity of KP1 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP1
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET-PLS-KP2-A66S_rpoA-U

    Plasmid

    #190957

    Purpose

    Test the U-to-C editing activity of KP2:A66S on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:A66S

    Promoter

    T7

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET-PLS-KP3-A66S_rpoA-U

    Plasmid

    #190961

    Purpose

    Test the U-to-C editing activity of KP3:A66S on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:A66S

    Promoter

    T7

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PM18033-PLS-KP7_rpoA-U

    Plasmid

    #190994

    Purpose

    Test the U-to-C editing activity of KP7 on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP7
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET-PLS-KP3-E67A_rpoA-U

    Plasmid

    #190963

    Purpose

    Test the U-to-C editing activity of KP3:E67A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:E67A

    Promoter

    T7

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PM18033-PLS-KP2-E67A_rpoA-U

    Plasmid

    #190979

    Purpose

    Test the U-to-C editing activity of KP2:E67A on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP2
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    DYW-KP:E67A

    Promoter

    CMV

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge