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pDS1902

Plasmid

#167278

Purpose

Expression plasmid for Candida albicans containing a red-shifted firefly luciferase

Depositor

Dominique Sanglard

Article

Dorsaz et al Front Microbiol. 2017 Aug 3;8:1478. doi:

Insert

red shifted luciferase

Promoter

ACT1

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRD323

Plasmid

#198044

Purpose

Expression of HMfA K31A E35A in E. coli

Depositor

Remus Dame

Article

Erkelens et al Front Microbiol. 2023 Apr 18;14:1166608.

Insert

HMfA K31A E35A

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDA12

Plasmid

#113663

Purpose

Cloning vector for bacterial expression with Pdhfr promoter

Depositor

Miguel Valvano

Article

Aubert et al Infect Immun. 2008 May;76(5):1979-91. do

Type

Empty backbone

Expression

Bacterial

Promoter

Pdhfr

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-uORF2-MET

Plasmid

#214105

Purpose

MET 5'UTR upstream of mGFP (uORF2 mutated)

Depositor

Dogus Altintas, Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

MET 5'UTR uORF2 mutated

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-Exon2-MET

Plasmid

#216536

Purpose

MET CDS without 5'UTR

Depositor

Dogus Altintas, Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

MET without 5'UTR

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA_MET_uORF1

Plasmid

#216537

Purpose

gRNA and template for MET 5'UTR uORF1 mutation

Depositor

Dogus Altintas, Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

pegRNA MET 5'UTR uORF1 mutation

Expression

Mammalian

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA_MET_uORF2

Plasmid

#216538

Purpose

gRNA and template for MET 5'UTR uORF2 mutation

Depositor

Dogus Altintas, Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

pegRNA MET 5'UTR uORF2 mutation

Expression

Mammalian

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-uORF1-MET

Plasmid

#214104

Purpose

MET 5'UTR upstream of mGFP (uORF1 mutated)

Depositor

Dogus Altintas, Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

MET 5'UTR

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

uORF1 mutation

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDL1531

Plasmid

#226637

Purpose

This plasmid is for correcting the nonsense mutation in the nrsB gene of several ICP1 isolates.

Depositor

Andrew Camilli

Article

Woldetsadik et al mBio. 2024 Oct 8:e0245724. doi: 10.1128/

Insert

Upstream sequence of nrsB (only 338bp total)

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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KNtp-SUR1 core fusion

Plasmid

#216212

Purpose

for expression of KNtp-SUR1 fusion protein in BacMam system

Depositor

Lei Chen

Article

Wang et al Front Pharmacol. 2022 Jun 30;13:929684.

Insert

SUR1 core

Tags

GFP-MBP

Expression

Mammalian

Mutation

deletion of TMD0

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pBN396

Plasmid

#109326

Purpose

Expresses FLP enzyme and fluorescent mNeonGreen in the C. elegans germ line

Depositor

Peter Askjaer

Article

Macias-Leon et al MicroPubl Biol. 2018 Mar 19;2018:10.1791

Insert

mex-5 (mex-5 Nematode)

Expression

Worm

Promoter

mex-5

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG039

Plasmid

#113996

Purpose

SC module. Terminator for T7RNAP

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Terminator for T7RNAP

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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Double UP mNeon to mScarlet

Plasmid

#125134

Purpose

Natively produces mNeon, in the presence of Cre will be recombined to produce mScarlet.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Type

Empty backbone

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Available Since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCON1-ProC.gfp

Plasmid

#112517

Purpose

ProC driven gfpmut3 expression with synTT

Depositor

Olivia Steele-Mortimer

Article

Cooper et al Front Cell Infect Microbiol. 2017 Nov 16

Insert

ProC gfpmut3 TT

Expression

Bacterial

Promoter

ProC

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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Double UP mScarlet to mNeon

Plasmid

#125139

Purpose

Natively produces mScarlet, in the presence of Cre will be recombined to produce mNeon.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Type

Empty backbone

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pEC2483 (pSpy1C)

Plasmid

#220248

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDS1928

Plasmid

#167284

Purpose

Expression plasmid for Candida albicans containing a red-shifted luciferase

Depositor

Dominique Sanglard

Article

Dorsaz et al Front Microbiol. 2017 Aug 3;8:1478. doi:

Inserts

red shifted luciferase
SAT1

Promoter

ACT1

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDA303

Plasmid

#195199

Purpose

Overexpressing 6His-TetR in E. coli.

Depositor

Rahul Sarpeshkar

Article

Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

Insert

TetR

Tags

6xHis

Expression

Bacterial

Promoter

T5 promoter

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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PCDNA3-PAR2-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

Plasmid

#113609

Purpose

PPI reporter

Depositor

Xiaokun Shu

Article

Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

Insert

PAR2

Expression

Mammalian

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pxpr_050-wt-ltr

Plasmid

#182646

Purpose

lentiviral expression of gRNA scaffold, contains intact LTR

Depositor

Jakob Reiser

Article

Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

Insert

None

Use

Lentiviral

Expression

Mammalian

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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