We narrowed to 18,395 results for: Tec

1. pBM13g-sd4U

   Plasmid

   #179689

   Purpose

   Bacterial expression of base editor

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   [sd4U] UGI-TALE3-DddA-G1397-N (S1330I + A1341V + N1342S + E1370K + T1380I)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S1330I A1341V N1342S E1370K T1380I

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1046E=dgRNAs[traB,bTub]

   Plasmid

   #149425

   Purpose

   The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes.

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

   Insert

   U6.3-gRNAs[TraB, bTub]
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6.3

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Prham-M.XhoI-M.MunI-M.Ocy1ORF8430P-p15A-aadA

   Plasmid

   #170768

   Purpose

   Methylase expression vector for 5RM assembly: expresses M.XhoI, M.MunI (MfeI) and M.Ocy1ORF8430P (SpeI) DNA methyltransferases in E. coli

   Depositor

   Ichiro Matsumura

   Article

   Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

   Inserts

   M.XhoI
   M.MunI
   M.Ocy1ORF8430P
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rhamnose

   Available since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-tdMCP2-GyG-(GGS)X6-HaloTag C261

   Plasmid

   #171434

   Purpose

   Expression of tdMCP2-GyG-(GGS)X6-HaloTag C261 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   tdMCP2-GyG-(GGS)X6-HaloTag C261
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lentiguide-Blast-eNMU710

   Plasmid

   #172658

   Purpose

   Expressing paired pegRNAs from human U6 and H1 promoters to make 710-bp deletion on e-NMU locus

   Depositor

   Jay Shendure

   Article

   Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

   Insert

   pegRNA-eNMUA/pegRNA-eNMUB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDB4961

   Plasmid

   #174015

   Purpose

   pHIS3H plasmid expressing 13Myc-Vps15 from 41nmt1 promoter

   Depositor

   Li-Lin Du

   Article

   Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

   Insert

   Vps15 (ppk19 Fission Yeast)
   

   Use

   Tags

   13Myc, Vps15

   Expression

   Yeast

   Mutation

   Promoter

   41nmt1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-HD252

   Plasmid

   #172656

   Purpose

   Expressing paired pegRNAs from human U6 and H1 promoters to make 252-bp deletion on HPRT1 gene

   Depositor

   Jay Shendure

   Article

   Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

   Insert

   pegRNA-HD252A/pegRNA-HD252B
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-pleCopyCatcher

   Plasmid

   #174061

   Purpose

   CopyCatcher insertion donor for the pale locus

   Depositor

   Ethan Bier

   Article

   Li et al Nat Commun. 2021 May 11;12(1):2625. doi:

   Insert

   Pale (Pale Synthetic, Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDB4953

   Plasmid

   #174020

   Purpose

   pDUAL plasmid expressing GFP-Atg6 from 41nmt1 promoter

   Depositor

   Li-Lin Du

   Article

   Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

   Insert

   Atg6
   

   Use

   Tags

   GFP, Atg6

   Expression

   Yeast

   Mutation

   Promoter

   41nmt1

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDB4658

    Plasmid

    #174017

    Purpose

    pDUAL plasmid expressing GFP-Atg8 from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    Atg8
    

    Use

    Tags

    GFP, Atg8

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDB4958

    Plasmid

    #174014

    Purpose

    pHIS3H plasmid expressing CFP-Atg6 from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    Atg6
    

    Use

    Tags

    CFP, Atg6

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDB4948

    Plasmid

    #174019

    Purpose

    pDUAL plasmid expressing Pil1-mCherry-Vps38 from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    Vps38
    

    Use

    Tags

    Pil1, mCherry, Vps38

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDB4946

    Plasmid

    #174018

    Purpose

    pDUAL plasmid expressing Pil1-mCherry-Atg14 from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    Atg14 (atg14 Fission Yeast)
    

    Use

    Tags

    Pil1, mCherry, Atg14

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDB4925

    Plasmid

    #174016

    Purpose

    pDUAL plasmid expressing Pil1-mCherry-Atg38(161-190) from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    Atg38(161-190)
    

    Use

    Tags

    Pil1, mCherry, Atg38(161-190)

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET19b-rDAD2 cPGFP(N180)

    Plasmid

    #166001

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rDAD2 cPGFP(N180)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LSSmOrange- V49A (please see depositor comments)

    Promoter

    T7 promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJIT163-AFID-2

    Plasmid

    #167358

    Purpose

    For inducing predictable deletions in protoplasts and wheat plants

    Depositor

    Caixia Gao

    Article

    Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

    Insert

    A3A-Cas9-UDG
    

    Use

    Tags

    Expression

    Plant

    Mutation

    WT

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLJM1-zeo-2xStrep-ORF68

    Plasmid

    #162659

    Purpose

    Lentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Lentiviral

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLJM1-zeo-2xStrep-UL52

    Plasmid

    #162663

    Purpose

    Lentiviral vector for stable expression of N-terminal 2xStrep-tagged UL52 in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    UL52
    

    Use

    Lentiviral

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA4/TO-2xStrep-ORF68 C415A

    Plasmid

    #162633

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 C415A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acid C415 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA4/TO-2xStrep-ORF68 H452A

    Plasmid

    #162634

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 H452A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acid H452 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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