We narrowed to 9,400 results for: Coli

1. pOPINK-OTUD6A (OTU, aa 128-288)

   Plasmid

   #61417

   Purpose

   Expresses human OTUD6A (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD6A (OTUD6A Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-127.

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-2T-TRR-C421-Y2404F

   Plasmid

   #182846

   Purpose

   mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG induction

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2014 Mar;141(5):1129-39. do

   Insert

   TRR 2011-2431 (trr Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…

   Promoter

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-6P-3-F37A+L196F+V252A+C253G-hADPRM

   Plasmid

   #107163

   Purpose

   Expresses specific cyclic-ADP-ribose (cADPR) phosphohydrolase in Escherichia coli as a GST fusion protein that can be cut with PreScission(TM) protease to yield active enzyme

   Depositor

   Jose Carlos Cameselle

   Article

   Ribeiro et al Sci Rep. 2018 Jan 18;8(1):1036. doi: 10.

   Insert

   F37A+L196F+V252A+C253G-hADPRM GST-mutant cADPR phosphohydrolase gene
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   March 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOPINK-OTUD6B (OTU, aa 133-293)

   Plasmid

   #61419

   Purpose

   Expresses human OTUD6B (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD6B (OTUD6B Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-132.

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAR69_STRI_m(KS_MAT)_H8_pET22b

   Plasmid

   #122849

   Purpose

   Expresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

   Depositor

   Martin Grininger

   Article

   Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

   Insert

   m(KS_MAT) (Fasn Mouse)
   

   Use

   Tags

   His8 tag and Twin-Strep tag

   Expression

   Bacterial

   Mutation

   wildtype

   Promoter

   T7 promoter

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQCascade-IS2

   Plasmid

   #140625

   Purpose

   Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS2. The crRNA-IS2 targets IS2 loci in Escherchia coli.

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS2
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET22b-ecDHFR-N23PP/L28F/G51PEKN

   Plasmid

   #118585

   Purpose

   Bacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

   Insert

   DHFR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N23 mutated to P23, L28 mutated to F28, G51 mutat…

   Promoter

   T7

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHelA12

   Plasmid

   #176493

   Purpose

   Expresses the N-terminal His6-tagged SARS-CoV helicase in E coli.

   Depositor

   Jian-Dong Huang, Xiaodong Cheng

   Article

   Tanner et al J Biol Chem. 2003 Oct 10;278(41):39578-8

   Insert

   SARS-CoV NSP13 (ORF1ab SARS-CoV)
   

   Use

   Tags

   His6 and T7

   Expression

   Bacterial

   Mutation

   V570I

   Promoter

   T7

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET29a-hNABP2-N

   Plasmid

   #128308

   Purpose

   Express hNABP2 N-terminus (1–138 aa) in E. coli with a C-terminal His-tag

   Depositor

   Yuliang Wu

   Article

   Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

   Insert

   hNABP2 N terminal fragment (OB fold) (NABP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   N-terminal domain (1–138 aa)

   Promoter

   T7

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOPINK-OTUD2 (OTU, aa 132-314)

    Plasmid

    #61410

    Purpose

    Expresses human OTUD2 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD2 (YOD1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-131 and aa 315-348.

    Promoter

    T7

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET29a-hNABP2NhNABP1C

    Plasmid

    #128313

    Purpose

    Express chimeric protein hNABP2 N-terminus and hNABP1 C-terminus in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    chimeric protein hNABP2N+hNABP1C (NABP2, NABP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    chimeric protein hNABP2N+hNABP1C, Y85C (please se…

    Promoter

    T7

    Available Since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRD404

    Plasmid

    #163109

    Purpose

    Expression of mNeonGreen_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    mNeonGreen_H-NSdbd
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. His 3C Kinase dead MKK6 1-334

    Plasmid

    #87882

    Purpose

    E. coli expression of MKK6 kinase dead

    Depositor

    Peter Mace

    Article

    Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Tags

    3C protease site and 6xHis

    Expression

    Bacterial

    Mutation

    K82A, kinase dead mutant

    Promoter

    T7

    Available Since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTrc99S-ssDsbA-PD-4xDQNAT

    Plasmid

    #128399

    Purpose

    Periplasmic expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    H. influenzae protein D
    

    Use

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. hSRAP.TWIN1.a13-69

    Plasmid

    #49815

    Purpose

    expression vector for SRAP(13-69) in pTWIN1 vector

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator protein aa 13-69 (SRA1 Human)
    

    Use

    Tags

    intein & chitin binding domain

    Expression

    Bacterial

    Mutation

    wildtype codon-optimized for E. coli expression

    Promoter

    T7

    Available Since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTAMAHISTEV_PfeAR480A-Q482A

    Plasmid

    #128946

    Purpose

    expresses R480A Q482A mutant of PfeA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

    Insert

    ferric enterobactin receptor
    

    Use

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-75), …

    Promoter

    T7

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p-OiRS3GG

    Plasmid

    #98589

    Purpose

    Expresses default target RNA (gI intron) and pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).

    Depositor

    Lydia Contreras

    Article

    Vazquez-Anderson et al Nucleic Acids Res. 2017 May 19;45(9):552

    Inserts

    group I intron
    PheS cassette to be selectively replaced by unique asRNA probes
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD and pLtetO

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX6P1-human LIC1 G domain

    Plasmid

    #74598

    Purpose

    used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
    

    Use

    Tags

    N-terminal GST and Strep II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. hSRAP.TWIN1.a13-87

    Plasmid

    #49816

    Purpose

    expression plasmid for SRAP(13-87) in pTWIN1 vector

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator protein aa 13-87 (SRA1 Human)
    

    Use

    Tags

    intein & chitin binding domain

    Expression

    Bacterial

    Mutation

    wildtype codon-optimized for E. coli expression

    Promoter

    T7

    Available Since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hSRAP.TWIN1.a13-215

    Plasmid

    #49817

    Purpose

    expression vector for SRAP(13-215) in pTWIN1 vector

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator protein aa 13-215 (SRA1 Human)
    

    Use

    Tags

    intein & chitin binding domain

    Expression

    Bacterial

    Mutation

    wildtype codon-optimized for E. coli expression

    Promoter

    T7

    Available Since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLIC-His6-MBP-TEV-Rgs14

    Plasmid

    #236395

    Purpose

    Expresses His6-MBP-TEV-Rgs14 in E. coli

    Depositor

    John Hepler

    Article

    Brown et al J Biol Chem. 2015 Apr 3;290(14):9037-49.

    Insert

    Rattus norvegicus regulator of G-protein signaling 14 (Rgs14 Rat)
    

    Use

    Tags

    hexahistidine (H6) tag and maltose-binding protei…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. WT USP7 UBL-12

    Plasmid

    #227230

    Purpose

    Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Article

    Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    aa 535-775

    Promoter

    T7-promoter

    Available Since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRSF-G1(7CNW)RS

    Plasmid

    #222874

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Cyano-L-Tryptophan (7-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRSF-G1(5CNW)RS

    Plasmid

    #222872

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET26b_AT118-H-V5-His

    Plasmid

    #222850

    Purpose

    E. coli periplasmic expression plasmid encoding Nb. AT118-A, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-H
    

    Use

    Tags

    V5-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28:LanIV-Streptomyces

    Plasmid

    #226250

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from Streptomyces LanIV (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanIV-Streptomyces)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. hzHDAC10

    Plasmid

    #225542

    Purpose

    Expresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.

    Depositor

    David Christianson

    Article

    Herbst-Gervasoni et al ACS Chem Biol. 2020 Aug 21;15(8):2154-21

    Insert

    Histone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)
    

    Use

    Tags

    His6-MBP tag with cleavable TEV site

    Expression

    Bacterial

    Mutation

    Mutated A24E and D94A

    Promoter

    T7

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28:LanII-2C

    Plasmid

    #225070

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanII-2C (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanII-2C)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28:LanII-2A

    Plasmid

    #225068

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanII-2A (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanII-2A)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28:LanII-2B

    Plasmid

    #225069

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanII-2B (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanII-2B)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge