We narrowed to 16,165 results for: igh

1. Dendra2-Actin-C-18

   Plasmid

   #57701

   Purpose

   Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   Actin (ACTB Human)
   

   Use

   Tags

   Dendra2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pRSFDuet-1_VNp-His6

   Plasmid

   #182386

   Purpose

   Bacterial expression of Vesicle Nucleating peptide

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-His
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mc2 mNeon hPGK

   Plasmid

   #206230

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. KillerOrange /pQE-30

   Plasmid

   #74748

   Purpose

   KillerOrange gene for bacterial expression

   Depositor

   Konstantin Lukyanov

   Article

   Sarkisyan et al PLoS One. 2015 Dec 17;10(12):e0145287. d

   Insert

   KillerOrange
   

   Use

   Tags

   HisTag

   Expression

   Bacterial

   Mutation

   KillerRed with substitutions G3C Y66W D113S N145S…

   Promoter

   T5

   Available since

   Aug. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-Camk2-NES-Twitch-GR

   Plasmid

   #173044

   Purpose

   TnC-based calcium sensor with green-red FRET pair ratiometric readout

   Depositor

   Yiyang Gong

   Article

   Zhang et al Commun Biol. 2021 Jul 29;4(1):924. doi:

   Insert

   NES-Twitch-GR
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CamK2

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMAH-POLY

   Plasmid

   #64915

   Purpose

   Encodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

   Depositor

   Huiwang Ai

   Article

   Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-

   Inserts

   Polyspecific aminoacyl-tRNA Synthetase
   Amber Suppression tRNA
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6/H1

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FU-tetO-Gateway

   Plasmid

   #43914

   Depositor

   John Gearhart

   Article

   Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector, doxycycli…

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet-ON

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV-REPORT (PGK) NanoLuc

   Plasmid

   #208576

   Purpose

   Empty NanoLuc Lentiviral reporter

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   NanoLuciferase
   d2eGFP
   Empty response element
   

   Use

   Lentiviral, Luciferase, and Synthetic Biology

   Tags

   3x SV40 NLS and PEST

   Expression

   Mammalian

   Mutation

   Promoter

   PGK and miniP

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiV2-EF1α-nCas9/RT

   Plasmid

   #210188

   Purpose

   Expression of nCas9/RT-P2A-Puro

   Depositor

   Yin Shen

   Article

   Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

   Insert

   nCas9/RT-P2A-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTPH418b

    Plasmid

    #185725

    Purpose

    Directed evolution of Cas9 adenine base editors with altered PAM compatibility

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    gIII(N)-NpuN-NpuC-gIII(C)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTPH412

    Plasmid

    #185724

    Purpose

    Directed evolution of Cas9 adenine base editors with altered PAM compatibility

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-gp41-8N
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    ecTadA(8e) R26G

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Dendra2-Golgi-7

    Plasmid

    #57722

    Purpose

    Localization: Golgi, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Golgi (B4GALT1 Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pAtetO_6xHis-Bst-LF

    Plasmid

    #145799

    Purpose

    Plasmid for the aTc-inducible expression of the large-fragment of Bst DNAP

    Depositor

    Andrew Ellington

    Article

    Bhadra et al mSphere. 2021 May 19;6(3):e00911-20. doi

    Insert

    Bst-LF
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    tet PA

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. LL - hNANOGi

    Plasmid

    #12196

    Depositor

    George Daley

    Article

    Zaehres et al Stem Cells. 2005 Mar . 23(3):299-305.

    Insert

    NANOG - RNAi (NANOG Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pACT5FVMF(f)

    Plasmid

    #110549

    Purpose

    Control plasmid for titering FV by qPCR to determine the genome-containing particle number. This qPCR protocol is described in Hendrie et al., 2008

    Depositor

    David Russell

    Article

    Hendrie et al Mol Ther. 2008 Mar;16(3):534-40. doi: 10

    Insert

    EGFP, FV LTRs
    

    Use

    Qpcr control for titering foamy virus

    Tags

    Expression

    Mutation

    Promoter

    promoter: MLV LTR

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-Camk2-NES-Twitch-NR

    Plasmid

    #173043

    Purpose

    TnC-based calcium sensor with green-red FRET pair ratiometric readout

    Depositor

    Yiyang Gong

    Article

    Zhang et al Commun Biol. 2021 Jul 29;4(1):924. doi:

    Insert

    NES-Twitch-NR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamK2

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmScarlet-H_NES_C1

    Plasmid

    #85061

    Purpose

    In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HcKCR2_mCherry_pcDNA3.1

    Plasmid

    #177337

    Purpose

    Expresses KCR2 in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

    Insert

    HcKCR2
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    mammalian codon-adapted

    Promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-mRuby3

    Plasmid

    #171154

    Purpose

    Encodes mRuby3 fluorophore

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    mRuby3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEBB HA cIAP1

    Plasmid

    #38232

    Depositor

    Colin Duckett

    Article

    Csomos et al Biochem J. 2009 Apr 28;420(1):83-91.

    Insert

    cIAP1 (BIRC2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLenti-CaMKIIa-C1C2-EYFP

    Plasmid

    #35519

    Depositor

    Karl Deisseroth

    Article

    Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

    Insert

    ChR1-ChR2 Chimera
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    June 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FU-tet-o-hSox2

    Plasmid

    #19779

    Depositor

    Konrad Hochedlinger

    Article

    Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

    Insert

    Sox2 (SOX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. human PNP non-tagged

    Plasmid

    #64199

    Purpose

    Expression of native human PNP (non-tagged)

    Depositor

    Vern Schramm

    Article

    Murkin et al Biochemistry. 2007 May 1;46(17):5038-49.

    Insert

    Purine Nucleoside Phosphorylase (PNP Human)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDGE655

    Plasmid

    #153253

    Purpose

    M1 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDB179-Z1-PETase

    Plasmid

    #218703

    Purpose

    Encodes codon optimized Z1-PETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    Z1-PETase
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Skp

    Plasmid

    #213139

    Purpose

    Expresses chaperone protein SKP

    Depositor

    Sebastian Hiller

    Article

    Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

    Insert

    SKP (21-161) (skp E.coli)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMK345 (Hygro-P2A-mAID-mClover)

    Plasmid

    #121179

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID-mClover
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Salsa-BC1-P1A1

    Plasmid

    #202842

    Purpose

    Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC1

    Depositor

    Noah Palm

    Article

    Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

    Insert

    EGFP/BC1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet Response Element (TRE)

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAC025

    Plasmid

    #227597

    Purpose

    Lentiviral backbone used when cloning pooled prime editing guide RNA libraries already containing a tevopreQ1 RNA motif

    Depositor

    Brittany Adamson

    Article

    Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SlugABEmax

    Plasmid

    #163798

    Purpose

    Expresses ABEmax and nSlugCas9

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    SlugCas9 (D10A)

    Promoter

    Available since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge