We narrowed to 7,174 results for: 11

1. lentiCRISPR v2-Nr4a1 double gRNAs

   Plasmid

   #162791

   Purpose

   Multiplex Guide RNAs to generate Nr4a1 knockout by CRISPR

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Multiplex Nr4a1 gRNA 1 and 2 (Nr4a1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available Since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1081)

   Plasmid

   #114094

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) nuclease with C-terminal NLS and HA tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a-RR (S542R/K607R)
   

   Use

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   S542R and K607R

   Promoter

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Fgf4enhLV (FpG12)

   Plasmid

   #69444

   Purpose

   positive control plasmid for lentiviral reporter expression in ESCs

   Depositor

   Lisa Dailey

   Article

   Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

   Inserts

   minimal FGF4 promoter
   EGFP
   Ubiquitin promoter
   Hygromycin resistance
   FGF4 enhancer (Fgf4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pINSPECT:IL2_SigP:NLuc

   Plasmid

   #197266

   Purpose

   Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human IL2 locus (exon 3). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

   Insert

   Interleukin-2 homology arms
   

   Use

   CRISPR, Luciferase, Synthetic Biology, and Unspec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184S

   Plasmid

   #106183

   Purpose

   Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Has Service

   AAV1

   Insert

   SF-Venus-iGluSnFR.A184S
   

   Use

   AAV

   Tags

   Expression

   Mutation

   SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

   Promoter

   hSynapsin

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV.CAG.SF-Venus-iGluSnFR.S72A

   Plasmid

   #106203

   Purpose

   Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-Venus-iGluSnFR.S72A
   

   Use

   AAV

   Tags

   Expression

   Mutation

   SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

   Promoter

   CAG

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.GFAP.SF-Venus-iGluSnFR.A184S

   Plasmid

   #106195

   Purpose

   Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-Venus-iGluSnFR.A184S
   

   Use

   AAV

   Tags

   Expression

   Mutation

   SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

   Promoter

   GFAP

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA216-CoV2-RBD-beta variant

   Plasmid

   #194598

   Purpose

   Mammalian expression of RBD domain from SARS-CoV-2 beta (B.1.351)

   Depositor

   Ben Murrell

   Article

   Sheward et al Cell Rep Med. 2021 Nov 16;2(11):100450.

   Insert

   CoV2-RBD-beta variant (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1065)

   Plasmid

   #114092

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a (S542R/K548V/N552R)
   

   Use

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   S542R, K548V and N552R

   Promoter

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pINSPECT:NEAT1-v2_SigP:NLuc

    Plasmid

    #197268

    Purpose

    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (long isoform only). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

    Insert

    NEAT1-v2 homology arms
    

    Use

    CRISPR, Luciferase, Synthetic Biology, and Unspec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.A184V

    Plasmid

    #106190

    Purpose

    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.A184V
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

    Promoter

    CAG

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV.hSynapsin.SF-Venus-iGluSnFR.S72A

    Plasmid

    #106179

    Purpose

    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.S72A
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

    Promoter

    hSynapsin

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-3xHA (AAS826)

    Plasmid

    #114091

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a (E174R/S542R)
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    E174R and S542R

    Promoter

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-EF1α1.1-Chronos-GFP

    Plasmid

    #122098

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α (1.1 kb short version)

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. shCCL20

    Plasmid

    #65096

    Purpose

    retroviral expression of CCL20 shRNA

    Depositor

    Pau Sancho-Bru

    Article

    Affo et al Gut. 2014 Nov;63(11):1782-92. doi: 10.11

    Insert

    Ccl20 shRNA (Ccl20 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.S72A

    Plasmid

    #106185

    Purpose

    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV1

    Insert

    SF-Venus-iGluSnFR.S72A
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

    Promoter

    hSynapsin

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.S72A

    Plasmid

    #106191

    Purpose

    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.S72A
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

    Promoter

    CAG

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A

    Plasmid

    #182049

    Purpose

    expression of Sox2 mutants

    Depositor

    Hyonchol Jang

    Article

    Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S248A/T258A

    Promoter

    CAG

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184V

    Plasmid

    #106184

    Purpose

    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.A184V
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

    Promoter

    hSynapsin

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc5.1B-EGFP-DmeIF4E

    Plasmid

    #79258

    Purpose

    Expresses EGFP-tagged DmeIF4E in S2 cells

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

    Insert

    eIF4E (eIF4E1 Fly)
    

    Use

    Schneider 2 cell expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Ac5 promoter

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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