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We narrowed to 5,233 results for: puc

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Showing: 5061 - 5080 of 5233 results

  5. pTS2333_Tier3(Lenti_inverse)-YPet_Puro

    Plasmid
    #169664
    Purpose
    Tier-3 vector for stable integration via lentiviral transduction in inverted direction with polyA carrying a constitutive expression cassette for puromyine and Ypet (PCMV-5'LTR-Psi-gag-env-PRPBSA-YPet-p2A-PuroR-pA::A2-pA::A3-pA-WPRE-3'LTR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven YPet and PuroR expression
    Expression
    Mammalian
    Promoter
    PhCMV / RPBSA
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169664-plasmid-map-sequence-id-365639

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  6. Bxb1Donor_G315T_ADRB2_LucReporter

    Plasmid
    #129793
    Purpose
    encodes a donor vector for recombination with the Bxb1 recombinase with variant G315T of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene
    Depositor
    Sriram Kosuri
    Article
    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1
    Insert
    ADRB2 (ADRB2 Human)
    Use
    Synthetic Biology
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    G315T
    Promoter
    N/A
    Available Since
    Sept. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    129793-plasmid-map-sequence-id-255170

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  7. Bxb1Donor_W99A_ADRB2_LucReporter

    Plasmid
    #129789
    Purpose
    encodes a donor vector for recombination with the Bxb1 recombinase with variant W99A of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene
    Depositor
    Sriram Kosuri
    Article
    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1
    Insert
    ADRB2 (ADRB2 Human)
    Use
    Synthetic Biology
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    W99A
    Promoter
    N/A
    Available Since
    Sept. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    129789-plasmid-map-sequence-id-255165

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  8. Bxb1Donor_N51L_ADRB2_LucReporter

    Plasmid
    #129790
    Purpose
    encodes a donor vector for recombination with the Bxb1 recombinase with variant N51L of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene
    Depositor
    Sriram Kosuri
    Article
    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1
    Insert
    ADRB2 (ADRB2 Human)
    Use
    Synthetic Biology
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    N51L
    Promoter
    N/A
    Available Since
    Sept. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    129790-plasmid-map-sequence-id-255172

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  9. pIFU002

    Plasmid
    #174299
    Purpose
    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the N501Y mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination
    Depositor
    Timothy Whitehead
    Article
    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:
    Insert
    Spike RBD N343Q, N501Y (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HA and cMyc
    Expression
    Yeast
    Mutation
    N343Q, N501Y
    Available Since
    Sept. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    174299-plasmid-map-sequence-id-342196

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  10. pIFU001

    Plasmid
    #174298
    Purpose
    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the E484K mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination
    Depositor
    Timothy Whitehead
    Article
    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:
    Insert
    Spike RBD N343Q, E484K (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HA and cMyc
    Expression
    Yeast
    Mutation
    N343Q, E484K
    Available Since
    Sept. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    174298-plasmid-map-sequence-id-342193

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  11. Ir7a-T2A-QF2 HDR plasmid

    Plasmid
    #140943
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7a gene
    Depositor
    Leslie Vosshall
    Article
    Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12
    Insert
    Ir7a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7a-right-HDR-arm
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140943-plasmid-map-sequence-id-313597

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  12. pVR2-m2

    Plasmid
    #84721
    Purpose
    pVR2 but with a G to U mutation in the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Expression
    Bacterial
    Mutation
    G to U mutation at position 16 of annotated "…
    Available Since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    84721-plasmid-map-sequence-id-167490

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  13. pVR2-m3

    Plasmid
    #84722
    Purpose
    pVR2 but with a C to G mutation in the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Expression
    Bacterial
    Mutation
    C to G mutation at position 22 of annotated "…
    Available Since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    84722-plasmid-map-sequence-id-167496

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  14. pZNF462-donor

    Plasmid
    #112380
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF462 with GFP
    Depositor
    Kevin White
    Insert
    ZNF462 homology arms flanking EGFP-IRES-Neo cassette (ZNF462 Human)
    Use
    CRISPR
    Mutation
    Unknown
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112380-plasmid-map-sequence-id-229749

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  15. pZNF41-donor

    Plasmid
    #112375
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF41 with GFP
    Depositor
    Kevin White
    Insert
    ZNF41 homology arms flanking EGFP-IRES-Neo cassette (ZNF41 Human)
    Use
    CRISPR
    Mutation
    hg38:X:47447496:G>T, hg38:X:47448269:C>A, h…
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112375-plasmid-map-sequence-id-229739

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  16. pZNF197-donor

    Plasmid
    #112357
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF197 with GFP
    Depositor
    Kevin White
    Insert
    ZNF197 homology arms flanking EGFP-IRES-Neo cassette (ZNF197 Human)
    Use
    CRISPR
    Mutation
    Unknown
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112357-plasmid-map-sequence-id-229676

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  17. pZBTB26-donor

    Plasmid
    #112356
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZBTB26 with GFP
    Depositor
    Kevin White
    Insert
    ZBTB26 homology arms flanking EGFP-IRES-Neo cassette (ZBTB26 Human)
    Use
    CRISPR
    Mutation
    hg38:9:122919395:A>G, hg38:9:122919458:T>G,…
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112356-plasmid-map-sequence-id-229674

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  18. pZNF639-donor

    Plasmid
    #112345
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF639 with GFP
    Depositor
    Kevin White
    Insert
    ZNF639 homology arms flanking EGFP-IRES-Neo cassette (ZNF639 Human)
    Use
    CRISPR
    Mutation
    hg38:3:179333542:G>T,rs978050614
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112345-plasmid-map-sequence-id-229659

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  19. pZNF589-donor

    Plasmid
    #112340
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF589 with GFP
    Depositor
    Kevin White
    Insert
    ZNF589 homology arms flanking EGFP-IRES-Neo cassette (ZNF589 Human)
    Use
    CRISPR
    Mutation
    hg38:3:48267877:T>C, hg38:3:48267890:C>T, h…
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112340-plasmid-map-sequence-id-229653

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  20. pELK3-donor

    Plasmid
    #112322
    Purpose
    CRISPR donor plasmid to tag human transcription factor ELK3 with GFP
    Depositor
    Kevin White
    Insert
    ELK3 homology arms flanking EGFP-IRES-Neo cassette (ELK3 Human)
    Use
    CRISPR
    Mutation
    hg38:12:96266960:A>T, rs760335647, rs6538704
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112322-plasmid-map-sequence-id-228533

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Showing: 5061 - 5080 of 5233 results