We narrowed to 18,567 results for: puro

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pL6puro-JeT-pmRevErbA-mClover3-NLS-Pest

Plasmid

#240109

Purpose

A lentiviral (yellow-green) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.

Depositor

Achim Kramer

Publication

Gabriel et al bioRxiv 2025.06.30.661926

Insert

murine Nr1d1 promoter+mClover3-NLS-Pest-Reporter (Nr1d1 Mouse)

Use

Lentiviral

Mutation

added JeT Promoter

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-hU6-enhanced_gRNA-BFP-P2A-PuroR

Plasmid

#230935

Purpose

gRNA cloning vector encoding BFP and puromycin resistance

Depositor

Charles Gersbach

Publication

Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

TagBFP

Promoter

hU6

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-hU6-cs1-PuroR-T2A-BFP

Plasmid

#230934

Purpose

gRNA cloning vector with direct capture sequence 1 in stem loop 2

Depositor

Charles Gersbach

Publication

Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

TagBFP

Available since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1a-Ccmut (N202P-V361P)

Plasmid

#225457

Purpose

Express mEGFP-fusion protein of FXR1 isoform a (N202P V361P)

Depositor

Christine Mayr

Publication

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

N202P V361P

Promoter

CMV

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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J135F_BattB_Vif(T27X4)-IRES-SpyTag-sfmCherry2(11)-PuroR

Plasmid

#200637

Purpose

Recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

Depositor

Kenneth Matreyek

Publication

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

Vif

Expression

Mammalian

Mutation

T27stop

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR

Plasmid

#206213

Purpose

PiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplification

Depositor

Masamichi Kamihira

Publication

Kitano et al Cytotechnology. 2021 Jun;73(3):353-362.

Insert

tetracycline-responsive transactivator

Expression

Mammalian

Available since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLPC-puromycin-binary-MDH1-3xFLAG-ME1- HA

Plasmid

#184549

Purpose

Retroviral vector to co-express human MDH1 with 3xFLAG tag and human ME1 with HA tag

Depositor

Gerardo Ferbeyre

Publication

Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

Inserts

Malate dehydogenase 1 (MDH1 Human)
Malic Enzyme 1 (ME1 Human)

Use

Retroviral

Tags

3xFLAG and HA tag

Expression

Mammalian

Promoter

CMV

Available since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP

Plasmid

#162336

Purpose

Lentiviral expression of sgNGN3 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Publication

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6; mU6

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLIX403_deltaUBA_UBAP2L_mCherry

Plasmid

#105287

Purpose

puro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein lacking the UBA domain of UBAP2L

Depositor

Eugene Yeo

Publication

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

UBAP2L (UBAP2L Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

deleted amino acids 20-108

Promoter

TRE promoter, Tet ON

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAVS1_TREtight-GEM22-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

Plasmid

#197064

Purpose

Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM22-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

Depositor

Julia Mahamid

Publication

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Inserts

Encapsulin
Adaptor Protein

Use

CRISPR

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pPD1060 Integration Vector with Puro-P2A-dsRedE2

Plasmid

#253873

Purpose

Integration Vector (PuroR-P2ADsRedExpress2)

Depositor

Joshua Leonard

Publication

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

P2ADsRedExpress2

Use

Synthetic Biology

Expression

Mammalian

Promoter

N/A

Available since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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pLEX307-puro-FlipGFP-SARS-CoV-2-3CLcs

Plasmid

#247687

Purpose

Lentivirus compatible constitutive expression of FlipGFP construct with SARS-CoV-2 3CL cut site (AVLQSGFR) with puromycin resistance marker.

Depositor

Sho Iketani

Publication

Chithelen et al Nat Commun. 2026 May 19;17(1):6575. doi:

Insert

FlipGFP with SARS-CoV-2 3CL protease cut site

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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pLEX307-puro-SARS-CoV-2-nsp5-C145A

Plasmid

#247692

Purpose

Constitutive expression of SARS-CoV-2 nsp5 with C145A mutation under EF1-alpha promoter with puromycin resistance.

Depositor

Sho Iketani

Publication

Chithelen et al Nat Commun. 2026 May 19;17(1):6575. doi:

Insert

SARS-CoV-2 3CL protease with C145A

Use

Lentiviral

Expression

Mammalian

Mutation

C145A

Promoter

EF1a

Available since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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pLEX307-puro-SARS-CoV-2-nsp5-T21I

Plasmid

#247693

Purpose

Constitutive expression of SARS-CoV-2 nsp5 with a T21I mutation under EF1-alpha promoter with puromycin resistance.

Depositor

Sho Iketani

Publication

Chithelen et al Nat Commun. 2026 May 19;17(1):6575. doi:

Insert

SARS-CoV-2 3CL protease with T21I

Use

Lentiviral

Expression

Mammalian

Mutation

T21I

Promoter

EF1a

Available since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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pLEX307-puro-SARS-CoV-2-nsp5-L50F

Plasmid

#247694

Purpose

Constitutive expression of SARS-CoV-2 nsp5 with a L50F mutation under EF1-alpha promoter with puromycin resistance.

Depositor

Sho Iketani

Publication

Chithelen et al Nat Commun. 2026 May 19;17(1):6575. doi:

Insert

SARS-CoV-2 3CL protease with L50F

Use

Lentiviral

Expression

Mammalian

Mutation

L50F

Promoter

EF1a

Available since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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pLEX307-puro-SARS-CoV-2-nsp5-E166V

Plasmid

#247695

Purpose

Constitutive expression of SARS-CoV-2 nsp5 with a E166V mutation under EF1-alpha promoter with puromycin resistance.

Depositor

Sho Iketani

Publication

Chithelen et al Nat Commun. 2026 May 19;17(1):6575. doi:

Insert

SARS-CoV-2 3CL protease with E166V

Use

Lentiviral

Expression

Mammalian

Mutation

E166V

Promoter

EF1a

Available since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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pLEX307-puro-SARS-CoV-2-nsp5-E166A

Plasmid

#247696

Purpose

Constitutive expression of SARS-CoV-2 nsp5 with a E166A mutation under EF1-alpha promoter with puromycin resistance.

Depositor

Sho Iketani

Publication

Chithelen et al Nat Commun. 2026 May 19;17(1):6575. doi:

Insert

SARS-CoV-2 3CL protease with E166A

Use

Lentiviral

Expression

Mammalian

Mutation

E166A

Promoter

EF1a

Available since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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pLEX307-puro-SARS-CoV-2-nsp5-S144A

Plasmid

#247697

Purpose

Constitutive expression of SARS-CoV-2 nsp5 with a S144A mutation under EF1-alpha promoter with puromycin resistance.

Depositor

Sho Iketani

Publication

Chithelen et al Nat Commun. 2026 May 19;17(1):6575. doi:

Insert

SARS-CoV-2 3CL protease with S144A

Use

Lentiviral

Expression

Mammalian

Mutation

S144A

Promoter

EF1a

Available since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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pLEX307-puro-SARS-CoV-2-nsp5-T304I

Plasmid

#247698

Purpose

Constitutive expression of SARS-CoV-2 nsp5 with a T304I mutation under EF1-alpha promoter with puromycin resistance.

Depositor

Sho Iketani

Publication

Chithelen et al Nat Commun. 2026 May 19;17(1):6575. doi:

Insert

SARS-CoV-2 3CL protease with T304I

Use

Lentiviral

Expression

Mammalian

Mutation

T304I

Promoter

EF1a

Available since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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pTwist-Lenti-CMV-Puro-FBXW7-R479Q

Plasmid

#258089

Purpose

Overexpression

Depositor

Alexis Jourdain

Insert

FBXW7 (FBXW7 Human)

Use

Lentiviral

Mutation

R479Q, prevents substrate recognition

Promoter

CMV

Available since

July 13, 2026

Availability

Academic Institutions and Nonprofits only

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