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pcDNA3.1-rsAKARev

Plasmid

#166231

Purpose

Expresses reversibly switchable A-kinase activity reporter with EV-linker (rsAKARev) in mammalian cells

Depositor

Peter Dedecker

Article

Roebroek et al Nat Commun. 2021 Mar 31;12(1):2005. doi:

Insert

Reversibly switchable A-kinase activity reporter with EV-linker

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b_4CL:P5SHb + STS:P6SHb

Plasmid

#139792

Purpose

expression of biosynthetic enzymes 4CL and STS in fusion with CC

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Inserts

4CL:P5SHb
STS:P6SHb

Expression

Bacterial

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET19b_4CL:P3S + STS:AP4S

Plasmid

#139795

Purpose

expression of biosynthetic enzymes 4CL and STS in fusion with CC

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Inserts

4CL:P3S
STS:AP4S

Expression

Bacterial

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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NmCherry-SpVasa

Plasmid

#66786

Purpose

expression of mCherry in Sea Urchin germ cells

Depositor

Amro Hamdoun

Article

Campanale et al Development. 2012 Feb;139(4):783-92. doi

Insert

Vasa

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mammalian

Promoter

SP6

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only
pB24

Plasmid

#61153

Purpose

Expression of Hnt1

Depositor

Charles Brenner

Article

Bieganowski et al J Biol Chem. 2002 Mar 29;277(13):10852-6

Insert

Hnt1 (HNT1 Budding Yeast)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-XIAPmut

Plasmid

#8341

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAPmut (XIAP Human)

Tags

GST

Expression

Bacterial

Mutation

H467A

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only
DPY-pNCS

Plasmid

#17365

Depositor

Stephen Goff

Article

Yuan et al J Virol. 2000 Aug . 74(16):7250-60.

Insert

MMLV proviral DNA

Mutation

deletion that removes 5 amino acids (DPPPY) from …

Available Since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
XE86 TM Delta N Beta-catenin GFP

Plasmid

#16833

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

TM Delta N beta-catenin GFP (ctnnb1.L Frog)

Use

Xenopus expression

Tags

GFP

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pFS331-USP44-catDead-HIS

Plasmid

#43903

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Tags

HIS

Expression

Mammalian

Mutation

C282A

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pC0.122

Plasmid

#119629

Purpose

Level 0 Part. CDS

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

sgRNA scaffold

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.203

Plasmid

#119604

Purpose

Level 0 part. TSS promoter

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

J23103_TSS

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.082

Plasmid

#119611

Purpose

Level 0 part. Terminator

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

TrrnB

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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p3994 pGEM HHR23A

Plasmid

#10863

Depositor

Peter Howley

Article

Kumar et al J Biol Chem 1997 May 23;272(21):13548-54

Insert

HHR23A (RAD23A Human)

Expression

Bacterial

Available Since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only
pRC02

Plasmid

#114681

Purpose

sfGFP.Strand10-Haloenzyme

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

sfGFP.Strand10-Haloenzyme

Tags

His10

Expression

Bacterial

Promoter

T7

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pC0.029

Plasmid

#119626

Purpose

Level 0 Part. CDS

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

AbREry

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.078

Plasmid

#119609

Purpose

Level 0 part. Terminator

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

Tcpc_operon

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.083

Plasmid

#119592

Purpose

Level 0 part. Promoter

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

J23119MH_V01

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBT195

Plasmid

#127843

Purpose

clone BE4max CBEs by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

BE4max without deaminase

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pRibo-Sec-APEX2

Plasmid

#111699

Purpose

Theophylline-inducible expression of periplasmic APEX2 from a mycobacterial shuttle plasmid (episomal)

Depositor

Jessica Seeliger

Article

Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

Insert

APEX2

Tags

V5

Expression

Bacterial

Promoter

hsp60-ribo

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIP-Laforin Myc R171H

Plasmid

#84671

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 24, 2016

Availability

Academic Institutions and Nonprofits only