We narrowed to 10,632 results for: t7

1. pRSETB-ro4GFP

   Plasmid

   #82368

   Purpose

   Inducible expression of redox sensitive GFP in bacteria

   Depositor

   S. James Remington

   Article

   Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

   Insert

   ro4GFP
   

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   C48S/S65T/Q80R/N149C/S202C

   Promoter

   T7

   Available Since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a-mAIM2-deltaPYD

   Plasmid

   #51535

   Purpose

   Express His6-tagged HIN domain of mAIM2 in bacteria

   Depositor

   Emad Alnemri

   Article

   Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

   Insert

   Aim2 (Aim2 Mouse)
   

   Tags

   His6 and T7

   Expression

   Bacterial

   Promoter

   lac operator

   Available Since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. sv272-HisMBP-TZAPZNF9-11

   Plasmid

   #87185

   Purpose

   bacterial expression of HisMBP-TZAPZNF9-11

   Depositor

   Eros Lazzerini Denchi

   Article

   Li et al Science. 2017 Jan 12. pii: aah6752. doi:

   Insert

   TZAP (ZBTB48 Human)
   

   Tags

   HisMBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. 7xNLS-pMJ915v2-sfGFP

   Plasmid

   #88922

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCellFree_G03 S100A1

   Plasmid

   #67048

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   S100A1 (S100A1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. 274_pSKB3-Venus-LUMP

   Plasmid

   #65976

   Purpose

   Bacterial expression of Venus-LUMP fusion with thrombin cleavage site, a FRET-based thrombin sensor

   Depositor

   Gerard Marriott

   Article

   Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

   Insert

   LUMP
   

   Tags

   Venus and thrombin site (11-residue linker that h…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15b-pknG

   Plasmid

   #102814

   Purpose

   Expression of PknG in E.coli

   Depositor

   Jean Pieters

   Article

   Walburger et al Science. 2004 Jun 18;304(5678):1800-4. E

   Insert

   pknG
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-11a (GB1 WT - A34F)

   Plasmid

   #136609

   Purpose

   Expresses GB1 WT mutant (A34F)

   Depositor

   Roberto Chica

   Article

   Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

   Insert

   GB1 WT mutant (A34F)
   

   Tags

   6x-Histag

   Expression

   Bacterial

   Mutation

   Mutation from GB1 WT: A34F

   Promoter

   T7 promoter

   Available Since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET20b- hTPI Lys13Arg

   Plasmid

   #50726

   Purpose

   overexpression of the human TPI1 Lys13Arg allele in E.coli

   Depositor

   Markus Ralser

   Article

   Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

   Insert

   Triosephosphate isomerase 1 (TPI1 Human)
   

   Tags

   6xHIS tag

   Expression

   Bacterial

   Mutation

   changed Lysine 13 to Argenine

   Promoter

   T7

   Available Since

   Feb. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pI12

    Plasmid

    #24273

    Depositor

    Mariano Garcia-Blanco

    Article

    Baraniak et al Mol Cell Biol. 2003 Dec . 23(24):9327-37

    Insert

    modified adenovirus exons and introns
    

    Use

    T7 for in vitro transcription

    Expression

    Mammalian

    Available Since

    March 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCellFree_G03 YY1

    Plasmid

    #67082

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    YY1 (YY1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. KaiC CI cat-/EE (E77Q; E78Q; S431E; T432E)

    Plasmid

    #41888

    Depositor

    Michael Rust

    Article

    Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

    Insert

    Kai C CI cat-/EE
    

    Expression

    Bacterial

    Mutation

    changed amin acids: 77,78,431 and 432 to glutamate

    Promoter

    t7

    Available Since

    March 19, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pCellFree_G03 PRKCA

    Plasmid

    #67115

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PRKCA Protein kinase C alpha type (PRKCA Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. TAL2406

    Plasmid

    #36809

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    JAK2-TALEN_Left (Jak2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

15. TAL3512

    Plasmid

    #47231

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-pcdh10a-left (pcdh10a Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pAAH1

    Plasmid

    #41825

    Purpose

    Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for hygromycin resistance.

    Depositor

    Melissa Moore

    Article

    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

    Type

    Empty backbone

    Use

    Pcr template

    Expression

    Bacterial and Yeast

    Promoter

    T7, TEF

    Available Since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. NmMetQ WT

    Plasmid

    #129647

    Purpose

    Expresses the mature metQ gene from Neisseria meningitides, without the signal sequence

    Depositor

    Douglas Rees

    Article

    Nguyen et al Protein Sci. 2019 Jul 26. doi: 10.1002/p

    Insert

    metQ Gene
    

    Tags

    10x Histidine Tag, Enterokinase, and pelB signal …

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET15b+PCNA1 WT

    Plasmid

    #66139

    Purpose

    Expresses the wild type of Sulfolobus solfataricus PCNA1

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 1
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. TAL2407

    Plasmid

    #36810

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    JAK2-TALEN_Right (Jak2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

20. hisSUMO-mZFP57

    Plasmid

    #87224

    Purpose

    Express hisSUMO tagged Zinc finger protein 57 F1-F3(mouse) in E. coli

    Depositor

    Gary Stormo

    Article

    Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

    Insert

    ZFP57 (Zfp57 Mouse)
    

    Tags

    hisSUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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